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EC number: 224-460-9 | CAS number: 4368-56-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From May 26 to August 15, 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Sodium 1-amino-4-(cyclohexylamino)-9,10-dihydro-9,10-dioxoanthracene-2-sulphonate
- EC Number:
- 224-460-9
- EC Name:
- Sodium 1-amino-4-(cyclohexylamino)-9,10-dihydro-9,10-dioxoanthracene-2-sulphonate
- Cas Number:
- 4368-56-3
- Molecular formula:
- C20H219N2O5S.Na
- IUPAC Name:
- sodium 4-(4-acetamidophenyl-1-amino)-1-amino-9,10-dioxoanthracene-2-sulphonate
- Test material form:
- solid: particulate/powder
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: TOP Velaz Ltd.
- Weight at study initiation: 150 g.
- Housing: plastic polypropylene cages T4, equipped with dry softwood chip which was sterilized in a hot air sterilizer HS 401 A/1 at 150°C for 90 minutes.
- Diet (e.g. ad libitum): standard commercially manufactured complete Mixed fodders ST-1, 10 g/animal/day.
- Water (e.g. ad libitum): drinking water according to CSN 757111 ad libitum.
- Acclimation period: one week.
ENVIRONMENTAL CONDITIONS
- Temperature: 22 ± 3°C.
- Humidity: 50 ± 15%
- Photoperiod: 12 hrs cycle dark/light.
- Other: fluorescent light.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 20% suspension - Doses:
- 7.943 g/kg, 8.913 g/kg, 10.00 g/kg, 12.59 g/kg
- No. of animals per sex per dose:
- 5 per sex per dose
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days.
- Frequency of observations: the animals were observed for clinical signs of intoxication immediately after application (after 30 minutes), 3 hours after application and once a day for 14 days.
- Frequency of weighing: the animals were weighed before the oral administration and during the observation period.
- Necropsy of survivors performed: yes.
- Other examinations performed: clinical diagnosis was focused on observation of the appearance of skin, fur, visible mucous membranes, nutritional status, mental activity, somatomotor activity, responses to stimuli, focusing on sensibility and reactivity, lacrimation, assessment function respiratory, digestive, urogenital and circulatory system. Organs and muscles were examined macroscopically. After dissection internal organs were judged according to their color, size, consistency and structure. If the post-mortem bladder is filled with urine, the urine were carried out biochemical tests indicative indicator strips Heptaphan focusing on the detection of proteins, blood sugars, ketones, bilirubin, urobilinogen and pH. - Statistics:
- LD50 is calculated by the probit method according to Bliss. Mortality data used and the frequency and level logarithmic doses were entered into a computer and analyzed by the program PROBIT from October 1991 VÚOS.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 9 476.1 mg/kg bw
- Based on:
- test mat.
- Sex:
- male/female
- Dose descriptor:
- LD0
- Effect level:
- ca. 7 943 mg/kg bw
- Based on:
- test mat.
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 6 633.27 mg/kg bw
- Based on:
- act. ingr.
- Mortality:
- Mortality is observed in doses higher than 7.943 g/kg.
- Clinical signs:
- other: After application of the logarithmic dose of 12.59 g / kg the following clinical symptoms of intoxication were observed: - appearance of skin and hair: blue coloration of the skin after application and smoot, shiny hair - nutritional status: good - appear
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- LD50 (Male/Female, Rats) = 9476.1 mg/kg b.w. (6633.27 mg/kg b.w. based on the active ingredient)
- Executive summary:
The substance has been tested for acute toxicity by oral route accordimg to the OECD Guideline 401. Forty Wistar rats were tested with the following dose: 7.943 g/kg, 8.913 g/kg, 10.00 g/kg, 12.59 g/kg.
After 14 days of observation period the rats showed weight loss in all the application doses. The clinical signs observed are: conjuntivitis, locomotion disorders due to ataxia, diarrhea, hypergie, hyperaesthesia and blue coloration of the skin.
The substance shows an LD50 for male and female rats over the period of 14 days equal to 9476.1 mg/kg b.w. (6633.27 mg/kg b.w. based on the active ingredient).
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