Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-945-6 | CAS number: 101-48-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 Jan - 23 Feb 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- adopted 21. Jul 1997
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Ministerium für Umwelt und Verkehr Baden-Württemberg, Stuttgart, Germany
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,1-dimethoxy-2-phenylethane
- EC Number:
- 202-945-6
- EC Name:
- 1,1-dimethoxy-2-phenylethane
- Cas Number:
- 101-48-4
- Molecular formula:
- C10H14O2
- IUPAC Name:
- (2,2-dimethoxyethyl)benzene
Constituent 1
Method
- Target gene:
- his operon
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- cofactor supplemented post-mitochondrial fraction (S9 mix), prepared from the livers of male Sprague-Dawley rats treated with Aroclor 1254 (500 mg/kg bw)
- Test concentrations with justification for top dose:
- Experiment 1
15, 50, 150, 500 and 1500 µg/plate with and without metabolic activation for TA100 and TA102
50, 150, 500, 1500 and 5000 µg/plate with and without metabolic activation for TA98, TA1535 and TA1537
Experiment 2
50, 150, 500, 1500 and 5000 µg/plate without metabolic activation for TA98, TA100, TA102 and TA1537
500, 1000, 1500, 3000 and 5000 µg/plate without metabolic activation for TA1535
50, 150, 500, 1500 and 5000 µg/plate with metabolic activation for all strains - Vehicle / solvent:
- - Vehicle/solvent used: DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- mitomycin C
- other: 2-aminoanthracene (2-AA)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 48 to 72 h
NUMBER OF REPLICATIONS: 3 replications each in 2 independent experiments
DETERMINATION OF CYTOTOXICITY
- Method: reduction in the number of revertant colonies and/or a diminution of the background lawn - Statistics:
- X²-test was used to estimate the statistical significance of the difference between the mean number of revertants in the negative controls and the plates at each dosage level. Mean values and standard deviation were calculated.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Remarks:
- in TA1535 without S9 mix
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- in TA102 at 1500 µg/plate without S9 mix and at 5000 µg/plate with S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: No precipitation occurred up to the highest investigated dose. - Remarks on result:
- other:
Any other information on results incl. tables
Table 1. Test results of Experiment 1 (plate incorporation).
With or without S9-Mix |
Test substance concentration (μg/plate) |
Mean number of revertant colonies per plate (average of 3 plates ± Standard deviation) |
||||
Base-pair substitution type |
Frameshift type |
|||||
TA100 |
TA1535 |
TA102 |
TA98 |
TA1537 |
||
– |
0 |
99 ± 8 |
25 ± 2 |
293 ± 31 |
31 ± 6 |
10 ± 5 |
– |
0 (DMSO) |
86 ± 2 |
24 ± 3 |
298 ± 19 |
26 ± 3 |
12 ± 2 |
– |
15 |
89 ± 14 |
- |
305 ± 13 |
- |
- |
– |
50 |
73 ± 8 |
25 ± 6 |
296 ± 21 |
32 ± 3 |
12 ± 3 |
– |
150 |
76 ± 5 |
32 ± 3 |
303 ± 14 |
42 ± 5 |
12 ± 4 |
– |
500 |
83 ± 5 |
38 ± 7 |
248 ± 31 |
33 ± 8 |
11 ± 5 |
– |
1500 |
97 ± 8 |
43 ± 8 |
221 ± 6T |
39 ± 3 |
12 ± 4 |
– |
5000 |
- |
47 ± 3** |
- |
36 ± 4 |
10 ± 4 |
Positive controls, –S9 |
Name |
NaN3 |
NaN3 |
MMC |
2-NF |
9-AA |
Concentrations (μg/plate) |
0.7 |
0.7 |
0.15 |
2.5 |
50 |
|
Mean No. of colonies/plate (average of 3 ± SD) |
503 ± 58 |
905 ± 41 |
755 ± 51 |
614 ± 15 |
606 ± 63 |
|
+ |
0 |
97 ± 10 |
10 ± 3 |
343 ± 28 |
21 ± 4 |
17 ± 5 |
+ |
0 (DMSO) |
88 ± 8 |
11 ± 3 |
340 ± 14 |
21 ± 1 |
14 ± 3 |
+ |
15 |
88 ± 3 |
- |
349 ± 20 |
- |
- |
+ |
50 |
84 ± 15 |
9 ± 2 |
342 ± 37 |
25 ± 3 |
11 ± 4 |
+ |
150 |
81 ± 5 |
10 ± 3 |
348 ± 12 |
22 ± 6 |
14 ± 5 |
+ |
500 |
81 ± 3 |
10 ± 2 |
324 ± 17 |
25 ± 4 |
15 ± 3 |
+ |
1500 |
85 ± 8 |
12 ± 1 |
305 ± 21 |
23 ± 4 |
17 ± 3 |
+ |
5000 |
- |
14 ± 3 |
- |
22 ± 3 |
15 ± 2 |
Positive controls, +S9 |
Name |
2-AA |
2-AA |
2-AA |
2-AA |
2-AA |
Concentrations (μg/plate) |
0.8 |
0.8 |
0.9 |
0.8 |
1.7 |
|
Mean No. of colonies/plate (average of 3 ± SD) |
627 ± 90 |
216 ± 47 |
655 ± 61 |
741 ± 94 |
374 ± 103 |
DMSO: Dimethylsulphoxide
NaN3: Sodium azide
2-AA: 2-aminoanthracene
9-AA: 9-aminoacridine
2-NF: 2-nitrofluorene
MMC: Mytomycin C
T: bacteriotoxic
**: Significantly different from control (p < 0.01)
Table 2. Test results of Experiment 2 (plate incorporation).
With or without S9-Mix |
Test substance concentration (μg/plate) |
Mean number of revertant colonies per plate (average of 3 plates ± Standard deviation) |
||||
Base-pair substitution type |
Frameshift type |
|||||
TA100 |
TA1535 |
TA102 |
TA98 |
TA1537 |
||
– |
0 |
201 ± 16 |
19 ± 3 |
326 ± 20 |
25 ± 4 |
8 ± 4 |
– |
0 (DMSO) |
158 ± 6 |
20 ± 2 |
292 ± 18 |
23 ± 5 |
10 ± 2 |
– |
50 |
140 ± 22 |
40 ± 3** |
348 ± 14 |
23 ± 3 |
8 ± 4 |
– |
150 |
174 ± 21 |
35 ± 6 |
304 ± 22 |
24 ± 3 |
10 ± 3 |
– |
500 |
140 ± 15 |
44 ± 5** |
303 ± 19 |
26 ± 5 |
10 ± 3 |
– |
1500 |
173 ± 18 |
43 ± 5** |
248 ± 20 |
21 ± 3 |
6 ± 3 |
– |
5000 |
132 ± 21 |
38 ± 10 |
138 ± 8T |
24 ± 3 |
9 ± 4 |
Positive controls, –S9 |
Name |
NaN3 |
NaN3 |
MMC |
2-NF |
9-AA |
Concentrations (μg/plate) |
0.7 |
0.7 |
0.15 |
2.5 |
50 |
|
Mean No. of colonies/plate (average of 3 ± SD) |
471 ± 91 |
844 ± 12 |
916 ± 68 |
772 ± 26 |
493 ± 174 |
|
+ |
0 |
171 ± 23 |
9 ± 6 |
365 ± 5 |
29 ± 3 |
15 ± 6 |
+ |
0 (DMSO) |
174 ± 6 |
9 ± 3 |
360 ± 13 |
25 ± 3 |
11 ± 3 |
+ |
50 |
176 ± 23 |
6 ± 2 |
353 ± 37 |
25 ± 3 |
11 ± 5 |
+ |
150 |
206 ± 14 |
10 ± 3 |
369 ± 3 |
30 ± 1 |
12 ± 5 |
+ |
500 |
215 ± 15 |
8 ± 3 |
328 ± 30 |
26 ± 3 |
12 ± 5 |
+ |
1500 |
189 ± 21 |
8 ± 3 |
305 ± 48 |
25 ± 6 |
13 ± 4 |
+ |
5000 |
210 ± 29 |
9 ± 4 |
253 ± 8T |
25 ± 4 |
12 ± 5 |
Positive controls, +S9 |
Name |
2-AA |
2-AA |
2-AA |
2-AA |
2-AA |
Concentrations (μg/plate) |
0.8 |
0.8 |
0.9 |
0.8 |
1.7 |
|
Mean No. of colonies/plate (average of 3 ± SD) |
647 ± 113 |
129 ± 11 |
457 ± 23 |
833 ± 43 |
248 ± 24 |
DMSO: Dimethylsulphoxide
NaN3: Sodium azide
2-AA: 2-aminoanthracene
9-AA: 9-aminoacridine
2-NF: 2-nitrofluorene
MMC: Mytomycin C
T: bacteriotoxic
**: Significantly different from control (p < 0.01)
In the concentration range investigated the test substance induced a significant and dose related increase in the mutation frequency of the tester strain TA1535 in the absence of metabolic activation.
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of this Ames test the test substance was mutagenic in TA1535 without metabolic activation.
- Executive summary:
A bacterial gene mutation assay with the test substance was performed in accordance with OECD Guideline 471 and in compliance with GLP (2001). In two independent experiments, the Salmonella typhimurium strains TA98, TA100, TA102, TA1535 and TA1537 were exposed to the test substance using the plate incorporation method. Based on the results of a pre-experiment, test substance concentrations of 15 to 5000 µg/plate were selected for the incubation with and without metabolic activation in the first experiment. In the second experiment, concentrations of 50 to 5000 µg/plate were selected for the incubation with and without metabolic activation. No precipitation occurred up to the highest investigated dose. In the absence and presence of metabolic activation the test substance was bacteriotoxic towards the strain TA102 at 1500 and 5000 µg/plate, respectively. In the concentration range investigated the test substance induced a significant and dose related increase in the mutation frequency of the tester strain TA1535 in the absence of metabolic activation. The results with the positive control substances confirmed the known reversion properties and specificity of the tester strains as well as the full activity of the metabolizing system. Under the conditions of this experiment, the test substance was mutagenic in the S. typhimurium strain TA1535 without metabolic activation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)