4-[4-[[2,5-dimethoxy-4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl]benzenesulphonic acid, sodium salt

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1979-11-09 to 1979-11-16

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412-L31

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

p-[4,5-dihydro-4-[[2-methoxy-5-methyl-4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]-3-methyl-5-oxo-1H-pyrazol-1-yl]benzenesulphonic acid, sodium salt

EC Number:

263-417-9

EC Name:

p-[4,5-dihydro-4-[[2-methoxy-5-methyl-4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]-3-methyl-5-oxo-1H-pyrazol-1-yl]benzenesulphonic acid, sodium salt

Cas Number:

62121-75-9

Molecular formula:

C20H22N4O11S3.xNa C20H(22-x)N4NaxO11S3; x<=2

IUPAC Name:

sodium 4-[4-(2-{2-methoxy-5-methyl-4-[2-(sulfonatooxy)ethanesulfonyl]phenyl}diazen-1-yl)-3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl]benzene-1-sulfonate

Test material form:

liquid - solid: mixture of

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: dilution in test water (according to Marking & Dawson, 1973)
- Controls: negative
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no

Test organisms

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

TEST ORGANISM
- Common name: golden orf
- Source: Paul Eggers, 2345 Hohenwestedt, Germany
- Length at study initiation (length definition, mean, range and SD): 5.8 - 7.8 cm, mean 6.4 cm, SD 0.4 cm, n=50
- Weight at study initiation (mean and range, SD): 2.0 - 3.7 g, mean 2.8 g, SD 0.35 g, n=50

ACCLIMATION
- Acclimation period: min. 14 d
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation: TetraMin, ad libitum

FEEDING DURING TEST
- Amount: no feeding

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

carbonate hardness: 6.4°d

Test temperature:

20°C

pH:

7.5 - 8.3

Dissolved oxygen:

7.8 - 8.4 mg/L

Nominal and measured concentrations:

Nominal: 0(control), 1, 10, 100, 500 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: aquaria
- Material, size, headspace, fill volume: glass, 40 x 25 x 30 cm³, volume 20 L
- Aeration: yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1 fish / 2 L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Chlorine: dechlorinated
- Culture medium different from test medium: yes, salts according to Marking and Dawson added to test water

OTHER TEST CONDITIONS
- Photoperiod: 12/12
- Light intensity: 700 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, behaviour, macroscopic pathology

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: no mortality observed

Details on results:

- Behavioural abnormalities: fish swam at the surface in highest treatment
- Other biological observations: no macroscopic changes found in gross pathology
- Mortality of control: no mortality
- Effect concentrations exceeding solubility of substance in test medium: no

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96-h LC50 in a test with the golden orf (Leuciscus idus melanotus) was found to be >500 mg/L.