Isobutyl vinyl ether

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to Guideline study with acceptable restrictions: no details about the test substance; exposure duration max. 2 h; no scoring 72 h after exposure; duration of observation period 8 days

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Isobutyl vinyl ether

EC Number:

203-678-8

EC Name:

Isobutyl vinyl ether

Cas Number:

109-53-5

Molecular formula:

C6H12O

IUPAC Name:

1-(ethenyloxy)-2-methylpropane

Details on test material:

Isobutylvinylether; no further data

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

2 male White Vienna rabbits,
mean body weight 3.4 kg
standard diets and water ad libitum
Source: GAUKLER, Offenbach, FRG

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated skin

Amount / concentration applied:

ca. 0.5 mL

Duration of treatment / exposure:

5 min or 2 h

Observation period:

Excess TS was washed off (Lutrol/water). Skin reactions were examined 24 & 48 h and 8 days post treatment.

Number of animals:

2

Details on study design:

A patch (2.5 x 2.5 cm²) soaked with TS was placed to the shaved, intact dorsal skin. It was covered with an inert foil and held in place by an adhesive tape (only 2 h). Contact periods were 5 minutes and 2 hours. Skin reactions were scored according to the table of Draize contained in the OECD TG 404.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

animal #1

Remarks:

2 h

Time point:

24/48 h

Score:

2.5

Max. score:

4

Reversibility:

not fully reversible within: slight edema on day 8 post treatment in both animals.

Results 2

Irritation parameter:

edema score

Basis:

animal #1

Remarks:

2 h

Time point:

24/48 h

Score:

2

Max. score:

4

Reversibility:

not fully reversible within: slight edema on day 8 post treatment in both animals.

Results 3

Irritation parameter:

erythema score

Basis:

animal #2

Remarks:

2 h

Time point:

24/48 h

Score:

3

Max. score:

4

Reversibility:

not fully reversible within: slight edema on day 8 post treatment in both animals.

Results 4

Irritation parameter:

edema score

Basis:

animal #2

Remarks:

2 h

Time point:

24/48 h

Score:

2

Max. score:

4

Reversibility:

not fully reversible within: slight edema on day 8 post treatment in both animals.

Results 5

Irritation parameter:

erythema score

Basis:

animal #1

Remarks:

5 min

Time point:

24/48 h

Score:

2.5

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Results 6

Irritation parameter:

edema score

Basis:

animal #1

Remarks:

5 min

Time point:

24/48 h

Score:

1.5

Max. score:

4

Reversibility:

fully reversible within: 8 days

Results 7

Irritation parameter:

erythema score

Basis:

animal #2

Remarks:

5 min

Time point:

24/48 h

Score:

3

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Results 8

Irritation parameter:

edema score

Basis:

animal #2

Remarks:

5 min

Time point:

24/48 h

Score:

2

Max. score:

4

Reversibility:

fully reversible within: 8 days

Any other information on results incl. tables

Irritation
Contact period 5 minutes:
Erythema: moderate to severe erythema at 24 and 48 hrs post
treatment developed into slight desquamation until day 8 in
both animals. 
Edema: well defined at 24 and 48 hrs post treatment, absent
on day 8 post treatment in both animals.

Contact period 2 hours:
Erythema: moderate to severe erythema at 24 and 48 hrs post
treatment developed into superficial necrosis until
day 8 in both animals. 
Edema: well defined at 24 and 48 hrs post treatment, slight
edema on day 8 post treatment in both animals.

Systemic toxicity
No signs noted.

Applicant's summary and conclusion

Interpretation of results:

irritating

Remarks:

Migrated information

Conclusions:

Score of erythema and edema were 2-3 in two rabbits 24 and 48 h after application of 0.5 mL undiluted test substance and occlusive exposure for 2 h.
Superficial necroses developed within 8 days following application.

Executive summary:

Two White Vienna rabbits receiced 0.5 mL undiluted test substance on the shaved skin. The exposure duration was 2 h under occlusive conditions (then washing off). The skin reactions were examined 24 & 48 h and 8 days post treatment. Score of erythema and edema were 2-3 in two rabbits 24 and 48 h after application, the effects were not reversible; superficial necroses developed within 8 days following application.