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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1980
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
ACUTE ORAL TOXICITY OF SELECTED FLAVOR CHEMICALS
Author:
Moran, E. J., Easterday, O.D., Oser, B. L.
Year:
1980
Bibliographic source:
DRUG AND CHEMICAL TOXICOLOGY, 3(3) , 249-258 (1980)

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
GLP compliance:
no
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2-ethyl-3-methylpyrazine
EC Number:
239-799-8
EC Name:
2-ethyl-3-methylpyrazine
Cas Number:
15707-23-0
Molecular formula:
C7H10N2
IUPAC Name:
2-ethyl-3-methylpyrazine

Test animals

Species:
rat
Strain:
other: FDRL
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Fasting period before study: fasted overnight prior treatment
- Diet: Purina Laboratory Chos, ad libitum
- Water: ad libitum

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
corn oil
Doses:
no data
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: closely observed for mortality and pharmacological effects on the day of dossing and daily thereafter for a total of 14 observation days; body weight determination prior to dosing
Statistics:
LD50 values were calculated by appropriate statistical methods, as determined by Miller and Tainter (1944).

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
600 mg/kg bw
Based on:
test mat.
95% CL:
501 - 699
Mortality:
no data
Clinical signs:
other: no data
Gross pathology:
no data

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Conclusions:
The oral LD50 value for the test substance was determined to be 600 mg/kg bw.
Executive summary:

An acute oral toxicity study was conducted on 10 rats (5 male and 5 female animals). After an overnight fasting period the animals were weighted and the test item was applied by gavage in corn oil. The animals were observed for a period of 14 days. The calculated LD50 for the test item was 600 mg/kg bw.