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EC number: 202-595-4 | CAS number: 97-62-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21/07/2011 to 04/10/2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Bois-de-Bay, Satigny, Switzerland
- Storage conditions: aerobic
- Storage length: sludge collected in the morning and used same day
- Preparation of inoculum for exposure: washed 3 times by centrifugation
- Dry weight suspended solids: diluted to 1.53 g/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 30 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Remarks:
- measuring pressure drop
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to OECD 301 guideline
- Test temperature: 22.3 - 22.9 °C
- pH: 7.6 at start of experiment
- pH adjusted: no
- Suspended solids concentration: 1.53 g/L (activated sludge)
TEST SYSTEM
- Culturing apparatus: Oxitop Control System by Wissenschaftlich-Technische Werkstätten (WTW), Weilheim, Germany
- Number of culture flasks/concentration: 6 flasks
* 2 flasks: inoculum + test substance 30 mg/L
* 2 flasks: inoculum
* 2 flasks: inoculum + reference substance 100 mg/L
- Measuring equipment:
- Test performed in closed vessels: yes
- Details of trap for CO2 and volatile organics if used: 2 sodium hydroxide pellets
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: no
STATISTICAL METHODS: not specified - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 64
- St. dev.:
- 1
- Sampling time:
- 12 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 74
- St. dev.:
- 1.5
- Sampling time:
- 28 d
- Details on results:
- The 10-day window criterion is fulfilled: more than 60 % is degraded within the 10-day window.
The test substance did not inhibit the intrinsic respiration of the inoculum. - Parameter:
- ThOD
- Value:
- 2.2 mg O2/g test mat.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test substance degrades more than 60 % within the 10-d window and should thus be regarded as readily biodegradable.
- Executive summary:
In this study, the ready biodegradability of the test substance was assessed according to OECD 301F guideline, manometric respirometry. In this study the activity of the inoculum was verified and confirmed using sodium benzoate as reference substance. The validity criteria were all met. The study concludes that the 10 -d window criterion is fulfilled for the test substance since 15 % biodegraded on day 2 and 64 % on day 12. After 28 days incubation, the test substance underwent 74 % biodegradation. Thus, the test substance should be regarded as readily biodegradable.
Reference
Biodegradation over time:
day | % biodegradation |
1 | 1 |
2 | 15 |
7 | 53 |
12 | 64 |
14 | 65 |
21 | 72 |
28 | 74 |
Erroneously high oxygen consumption was observed for one of the flasks with reference substance. The results of this flask were not considered. Still this is not considered as guideline deviation as a single procedure control is sufficient according to OECD 301F.
Validity criteria:
Difference between replicate values < 20 % (i.e. maximally 4%) + Oxygen uptake in 28 days < 60 mg/L (i.e. maximally 38 %) + Degradation of reference substance exceeded 40 % after 7 days and 65 % after 14 days, activity of inoculum was thus verified.
Description of key information
The test substance should be regarded readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
For this endpoint, one study is available (Kreutzer, 2011) in which the readily biodegradability of the test substance is assessed according to OECD 301F. The study results show that 67 % of the test substance is biodegraded after 28 days and that the 10-d window criterion is fulfilled.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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