[2-[[4-[(2-chloro-4-nitrophenyl)azo]phenyl]ethylamino]ethyl](2-hydroxypropyl)dimethylammonium chloride

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 02, 2015 - July 10, 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: guideline study according to (EC A.6. and OECD 105)

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

GLP compliance:

no

Type of method:

flask method

Key result

Water solubility:

132 g/L

Conc. based on:

test mat.

Temp.:

20 °C

pH:

6

 Individual results

 

Preliminary visual estimation of the water solubility

In a stepwise procedure, increasing volumes of distilled water were added at room temperature to a defined amount of the test item. After each addition of an amount of water the mixture was treated with ultrasound for 15 minutes and visually checked for any undissolved particles.

Table 3: Results of the preliminary visual estimation of the water solubility

Amount of test item [mg]		Added volume of dist. water [ml]		Total volume [ml]		Appearance of mixture
93.0		0.1		0.1		non dissolved
		0.4		0.5		dissolved

The preliminary visual experiments showed that the water solubility of the test item was approximately 186 mg/L. According to the preliminary visual estimation in the main test, the water solubility was determined by the flask method.

Flask method

The data for the evaluation are given in Table 4. After the equilibration time a paste-like mass resulted. After centrifugation a liquid supernatant and large amounts of precipitate were observed in the flasks of the experiments excluding in the blank experiment. Only the supernatant was used for further analysis.The water solubility was calculated from the measured and unrounded concentration values and not from the rounded values given in Table 4.

Table 4: Water solubility experiments of the test item

Experiment	24 h experiment	48 h experiment	72 h experiment	Blank experiment (72 h)
Amount of test item [g]	6.05	6.11	6.02	--
Amount of distilled water [mL]	10	10	10	10
Stirring time at 30 °C [h]	24	48	72	72
pH at 22 °C	5.7	5.8	5.7	5.6
Aliquot of the flasks [mL]	0.5	0.5	0.5	0.5
1^stDilution to volume [mL]	50	50	50	50
Aliquot of the flasks [mL]	0.1	0.1	0.1	0.1
2^ndDilution to volume [mL]	5	5	5	5
Dilution factor	5000	5000	5000	5000
Measured concentration [mg/L]	23.82 / 23.92	30.16 / 30.10	25.05 / 25.19	-- / --
Mean concentration [mg/L]	23.87	30.13	25.12	--
Water solubility [g/L]	119.3	150.7	125.6	--

The water solubility of the test item at 20 °C was determined to be 132 g/L (± 19 g/L). This water solubility is only valid for the main component of the test item (no correction for its purity was done).

 

Conclusions:

The test item has a water solubility of 132 g/L (± 19 g/L) at 20 °C and can be considered highly soluble.

Executive summary:

The water solubility c_Sof the test item at a temperature of 20 °C was determined according to the flask method described in the European Commission Regulation (EC) No. 440/2008 and in the OECD 105. It was found to be:

                                                                 c_S=132 g/L (± 19 g/L)

Description of key information

The water solubility of the test item at a temperature of 20 °C as determined in accordance with methods EU A.6. and OECD 105 is: c_S=132 g/L (± 19 g/L).

Key value for chemical safety assessment

Water solubility:

132 g/L

at the temperature of:

20 °C