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EC number: 219-342-9 | CAS number: 2420-87-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08-03-2016 to 18-07-2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- 2008
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- Inoculum is of the aqueous phase of non-adapted activated sludge from the municipal sewage treatment plant in Hildesheim, Germany. The activated sludge was washed twice with chlorine free tap water. After the second washing the settled sludge was re-suspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2 hours. Thereafter the sludge was homogenized with a blender. After sedimentation the supernatant was decanted and maintained in an aerobic condition by aeration with air for 5 days. 10 mL/L were used to initiate inoculation.
Colony forming units in the test vessels: approx. 107 - 108 CFU/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 48 mg/L
- Based on:
- ThOD
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- The test solutions were prepared in measuring flasks and were placed in brown glass bottles as incubation vessels (inoculum control and functional control) or were prepared directly in brown glass bottles (test item and toxicity control):
• two for the inoculum control (C1, C2)
• one for the functional control (R1)
• two for the test item (P1, P2)
• one for the toxicity control (T1)
Separate replicates of test item and toxicity control were prepared for measurement of the pH-value at test start:
• one for the test item (PpH)
• one for the toxicity control (TpH)
The inoculated test medium, consisting of the required volumes of mineral medium stock solutions, ultrapure water and inoculum, were prepared in a measuring flask. 250 mL of this solution were filled in the brown glass bottles of the inoculum control, using a 250 mL measuring flask.
For the functional control, the reference item was weighed out and transferred into a measuring flask, the required volumes of mineral medium stock solutions, ultrapure water and inoculum, were added. 250 mL of this solution was filled in the brown glass bottle of the functional control, using a 250 mL measuring flask.
For the test item replicates the test item was weighed out for each replicate and directly transferred into the test vessel with 250 mL inoculated medium, using a 250 mL measuring flask.
For the toxicity control, the test item was weighed out and directly transferred into the test vessel with 250 mL of the reference item medium (see above), using a 250 mL measuring flask. - Reference substance:
- benzoic acid, sodium salt
- Test performance:
- Toxicity Control: In the toxicity control the biodegradation achieved 47% after 14 days. After 28 days the biodegradation came to 50%. The validity criterion that the test item should not inhibit the biodegradation of the reference item was fulfilled
The validity criteria were fulfilled according to the guideline:
• The percentage degradation of the functional control reached the pass level of 60 % within 3 days (validity criterion: 60 % after 14 days).
• The mean oxygen depletion in the inoculum control was 7.7 mg oxygen/L on day 28 (validity criterion: < 60 mg oxygen/L).
• The pH-value of all test replicates was in the range of 6.0 - 8.5 at the end of the test.
• The difference of extremes of replicate values of removal of the test item at the end of the test was less than 20 % (3 % difference on day 28).
• The percentage degradation of the toxicity control reached the pass level of 25 % within 2 days (validity criterion: 25 % after 14 days). - Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 2
- Sampling time:
- 28 d
- Results with reference substance:
- Functional control: The pass level for ready biodegradation (> 60% degradation) was reached within 3 days. The validity criterion that the degradation should be > 60% after 14 d was fulfilled. The adaptation phase changed to degradation phase within 1 day (degradation > 10%) and the biodegradation came to a maximum of 100% on day 13.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Both test item replicates did not reach the 10% level (beginning of biodegradation) until test end. The mean biodegradation on day 28 was 2%. The test item is classified as not readily biodegradable within the 28 day period of the study
- Executive summary:
The ready biodegradability of the test item was determined with non-adapted activated sludge in the Manometric Respirometry Test for a period of 28 days. The study was conducted according to OECD guideline 301 F and EC D.4-D. The test item concentration selected as appropriate was 48 mg/L, corresponding to a ThOD of 75.8 mg O2/L per test vessel. The degradation was followed by measuring the oxygen concentration. The mean oxygen depletion in the inoculum control was 7.7 mg O2/L on day 28.
In order to check the activity of the test system sodium benzoate was used as functional control. The pass level > 60% was reached within 3 days. The biodegradation reached a maximum of 100 % degradation on day 13.
In the toxicity control containing both test and reference item 47% degradation occurred within 14 days. After 28 days the biodegradation came to 50%. The degradation of the reference item was not inhibited by the test item.
The mean biodegradation on day 28 was 2%. The validity criteria of the guideline are fulfilled. The test item is classified as not readily biodegradable within the 28 day period of the study.
Reference
Description of key information
The ready biodegradability of the test item was determined with non-adapted activated sludge in the Manometric Respirometry Test for a period of 28 days. The study was conducted according to OECD guideline 301 F and EC D.4-D. The test item concentration selected as appropriate was 48 mg/L, corresponding to a ThOD of 75.8 mg O2/L per test vessel. The degradation was followed by measuring the oxygen concentration. The mean oxygen depletion in the inoculum control was 7.7 mg O2/L on day 28.
In order to check the activity of the test system sodium benzoate was used as functional control. The pass level > 60% was reached within 3 days. The biodegradation reached a maximum of 100 % degradation on day 13.
In the toxicity control containing both test and reference item 47% degradation occurred within 14 days. After 28 days the biodegradation came to 50%. The degradation of the reference item was not inhibited by the test item.
The mean biodegradation on day 28 was 2%. The validity criteria of the guideline are fulfilled. The test item is classified as not readily biodegradable within the 28 day period of the study.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
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