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EC number: 213-203-6 | CAS number: 929-59-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 5, 1985 - March 18, 1985
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3,6-dioxaoctamethylenediamine
- EC Number:
- 213-203-6
- EC Name:
- 3,6-dioxaoctamethylenediamine
- Cas Number:
- 929-59-9
- Molecular formula:
- C6H16N2O2
- IUPAC Name:
- 2-[2-(2-aminoethoxy)ethoxy]ethan-1-amine
- Test material form:
- liquid
- Details on test material:
- - Physical state: liquid
- Appearance: colourless liquid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 5601-49-1
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:Room temperature in container received
- Stability under test conditions: Stable
- Solubility and stability of the test substance in the solvent/vehicle: Soluble and stable
Method
- Target gene:
- Histidine locus
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA1535, TA1537, TA1538, TA98, TA100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9
- Test concentrations with justification for top dose:
- 50, 166, 500, 1666, and 5000 µg/plate.
- Vehicle / solvent:
- Distilled deonized water
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- Remarks:
- Negative controls without activation
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration:48-72 h at 37 degrees C
NUMBER OF REPLICATIONS: 3
- OTHER: 2 ml aliquots of molten top agar, 0.5 ml biotin and histadine, 0.1 ml tester strain, 0.1 ml test compound concentration were vortexed and poured onto minimal glucose plates in a thin layer. Plates needing activation were suplimented with 0.5 ml S-9 rat liver homogenate. Revertant colonies were counted with an electronic colony counter interfaced with a computer. - Evaluation criteria:
- A positive result is a dose-related significant increase in the number of histidine dependent colonies as determined by the program developed by Fenton and Moore. A negative result is the absence of reproducible increase in the number of histidine dependent colonies.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Untreated negative controls validity:
- not valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Untreated negative controls validity:
- not valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Untreated negative controls validity:
- not valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Untreated negative controls validity:
- not valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Untreated negative controls validity:
- not valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- The test material was not found to increase mutation frequencies in any strain of S. typhimurium, either with or without metabolic activation. The substance is considered to not be mutagenic.
- Executive summary:
The test article was received as a clear liquid. The solvent used throughout the assay was distilled/deionized water and 5000 microgram/plate.
Dose levels in a preliminary toxicity screen were 50, 166, 500, 1666 and 5000 microgram/plate. Strains TA1538 and TA100 of Salmonella typhimurium exhibited no inhibition of bacterial lawn growth at any of the dose levels tested.
Therefore , the top dose selected for the plate incorporation mutation assay was 5000 microgram/plate.
The test article was evaluated in strains TA 1535, TA1537, TA 1538 and TA100 of Salmonelle typhimurium both with and without metabolic activation preparation at dose of 50, 166, 500, 1666 and 5000 microgram/plate.
There were 0.10 mL of S-9 supernatant (31.1 mg protein/ml) per 1.0 ml of S9 -mix in the rat liver metabolic activation preparation.
The test article was negative in strains TA 1535, TA 1537, TA 1538, TA98 and TA100 of Salmonella typhimurium with and without metabolic activation preparation at dose of 50, 1661 500, 166 and 5000 microgram/plate. All solvent and positive controls used in the evaluation of the test article were within the acceptable limits of mean historical data.
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