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EC number: 406-690-3 | CAS number: 43136-14-7 MDI/ODA
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- Adopted 4th April 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Version / remarks:
- Adopted 30th May 1988
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No. of test material: 7ET2090
- Expiration date of the lot/batch: 31 December 2007
- Purity: 99 %
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At room temperature in the dark
- Stability under test conditions: Stable - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item was not soluble, so test concentrations were prepared separately in Milli-RO water. An initial loading rate of 200 mg/L was magnetically stirred for at least 24 hours. After 24 hours, the synthetic sewage feed and sludge were added. A final loading rate of 100 mg/L was tested in duplicate. The test solutions were continuously stirred during the test, to ensure optimal contact between the test item and the test medium. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Details on inoculum
- Source of inoculum/activated sludge: An activated sludge freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Preparation of inoculum for exposure: Before use the sludge was coarsely sieved, washed and diluted with ISO-medium. In order to determine the number of suspended solids a small proportion of the sludge was weighed and dried overnight in an oven at 105°C. After collection, 50 ml of synthetic sewage feed was then added per litre of activated sludge. The sludge was kept aerated under test conditions until use.
- Concentration of suspended solids: 3.3 g/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 18.0 - 18.8 ⁰C
- pH:
- 7.9 - 8.0
- Nominal and measured concentrations:
- Nominal test item concentration: 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:1 litre glass bottle.
- Aeration: Test media were aerated and stirred continuously
- No. of vessels per concentration (replicates): 2 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- - Blank controls oxygen uptake rate: No inhibition (0 %) was observed in the blank controls.
- Results with reference substance (positive control):
- - Results with reference substance valid? The EC50 of the reference substance was within the accepted range of 5-30 mg/L (9 mg/L)
- Reported statistics and error estimates:
- None
- Validity criteria fulfilled:
- yes
- Remarks:
- The EC50 of the reference substance was within the accepted range of 5-30 mg/L (9 mg/L); the difference between the negative controls (mean respiration rate) was within 15% of each other (12% and 3 %),
- Conclusions:
- Under test conditions, the test item was not toxic to activated sludge bacteria at loading rate of 100 mg/L. Therefore, the NOEC and EC50 are considered to be ≥100 and >100 mg/L, respectively.
- Executive summary:
Migchielsen (2006) is a GLP-compliant study following OECD guideline 209. The study is reliable without restrictions (Klimisch 1) as it followed a standard method. A blank control and reference item control were included in the test setup. As the test item was not soluble test concentrations were prepared separately. The test solutions were continuously stirred in a 1L glass bottle during the test, to ensure optimal contact between the test item and the test medium. After contact time the oxygen consumption of each sample was tested in a 300 mL oxygen bottle.
The EC50 of the reference substance was within the accepted range of 5-30 mg/L (9 mg/L); the difference between the negative controls (mean respiration rate) was within 15% of each other (12% and 3 %), therefore meeting the acceptability criteria of the test.
Under test conditions, the test item was not toxic to activated sludge bacteria at loading rate of 100 mg/L. Therefore, the NOEC and EC50 were ≥100 and >100 mg/L, respectively.
Reference
Table 1- Controls (C) and test substance (T) : pH, oxygen concentration, oxygen consumption and percentage inhibition
Flask |
Concentration (mg/L) |
Oxygen concentration at the start (mg O2/L) |
Oxygen consumption mg O2/L |
% Inhibition |
pH |
C3 |
0 |
8.0 |
38 |
|
8.0 |
C4 |
0 |
8.1 |
37 |
|
8.0 |
Mean C3 + C4 |
|
|
37.5 |
|
|
T1 |
100 |
7.6 |
39 |
-7 |
7.9 |
T2 |
100 |
7.7 |
40 |
-7 |
8.0 |
Description of key information
Under test conditions, the test item was not toxic to activated sludge bacteria at loading rate of 100 mg/L. Therefore, the NOEC and EC50 are considered to be ≥100 and >100 mg/L, respectively.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
Migchielsen (2006) is a GLP-compliant study following OECD guideline 209. The study is reliable without restrictions (Klimisch 1) as it followed a standard method. A blank control and reference item control were included in the test setup. As the test item was not soluble test concentrations were prepared separately. The test solutions were continuously stirred in a 1L glass bottle during the test, to ensure optimal contact between the test item and the test medium. After contact time the oxygen consumption of each sample was tested in a 300 mL oxygen bottle.
The EC50 of the reference substance was within the accepted range of 5-30 mg/L (9 mg/L); the difference between the negative controls (mean respiration rate) was within 15% of each other (12% and 3 %), therefore meeting the acceptability criteria of the test.
Under test conditions, the test item was not toxic to activated sludge bacteria at loading rate of 100 mg/L. Therefore, the NOEC and EC50 were ≥100 and >100 mg/L, respectively.
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