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EC number: 204-980-2 | CAS number: 130-20-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 21 - 23 February 1974
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Remarks:
- No information on negative control available.
- Qualifier:
- no guideline available
- Version / remarks:
- The test was conducted according to SOP A-11 (1977-09-01).
- Principles of method if other than guideline:
- - Principle of test: Fish are exposed to different solutions of the test substance.
- Short description of test conditions: 8-10 fish are used per concentration. Synthetic water is used, test concentrations are fixed.
- Parameters analysed / observed: Mortality (LC0, LC100), LC50 derivation is performed graphically and/or mathematically. - GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- not specified
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: dilution of a stock solution with synthetic water according to ISO norms
- Differential loading: 100, 200, 1000 mg/L
- Controls: positive control, solvent control if solvent used
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Methyl cellulose (2%) or ethanol (0.3%) or acetone (0.6%) if not water soluble - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout
- Source: P. Hohler, 4314 Zeiningen, Germany
- Age at study initiation: born in summer 1973
- Length at study initiation: 4-8 cm
- Weight at study initiation: 3-6 g
ACCLIMATION
- Acclimation period: min. 12 d
- Acclimation conditions (same as test or not): 10-13°C in flow-through aquaria with dechlorinated tap water, O2 min. 6.4 mg/L, day/night 12/12 h
- Type and amount of food during acclimation: Tetramin flakes
- Feeding frequency during acclimation: daily
FEEDING DURING TEST
- Frequency: no feeding during test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 12°C
- pH:
- 8.0 - 8.2
- Dissolved oxygen:
- >4.8 mg/L
- Nominal and measured concentrations:
- Nominal: 100, 200, 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- No. of organisms per vessel: 8
- No. of vessels per concentration (replicates): 1
TEST SYSTEM
- Test vessel: aquaria
- Material, size, headspace, fill volume: glass, 36 x 23 x 26 cm (17 L)
- Aeration: yes
- No. of organisms per vessel: 8
- No. of vessels per concentration (replicates): 1
- No. of vessels per vehicle control (replicates): 1 (if applicable)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Culture medium different from test medium: yes
OTHER TEST CONDITIONS
- Adjustment of pH: yes (if not in range of 6 - 8.5)
- Photoperiod: 12/12 h
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality at 24, 48, 72 and 96 h. 72 and 96 h only observed if effects observed at earlier test stages. - Reference substance (positive control):
- yes
- Remarks:
- Fish are randomly tested for sensitivity 6 d after arrival from the source (test substance: HgCl2).
- Duration:
- 48 h
- Dose descriptor:
- LC0
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
Not acutely toxic to fish with water from effluent (wastewater treatment plant) diluted by factor 5.
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The 48-h LC50 to fish (Oncorhynchus mykiss) was determined to be >1000 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 16 - 18 December 1977
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Remarks:
- No information on negative control available.
- Qualifier:
- no guideline available
- Version / remarks:
- The test was conducted according to SOP A-11 (1977-09-01).
- Principles of method if other than guideline:
- - Principle of test: Fish are exposed to different solutions of the test substance.
- Short description of test conditions: 8-10 fish are used per concentration. Synthetic water is used, test concentrations are fixed.
- Parameters analysed / observed: Mortality (LC0, LC100), LC50 derivation is performed graphically and/or mathematically. - GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- not specified
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: dilution of a stock solution with synthetic water according to ISO norms
- Differential loading: 100, 200, 1000 mg/L
- Controls: positive control, solvent control if solvent used
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Methyl cellulose (2%) or ethanol (0.3%) or acetone (0.6%) if not water soluble - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout
- Source: P. Hohler, 4314 Zeiningen, Germany
- Age at study initiation: born in summer 1973
- Length at study initiation: 4-8 cm
- Weight at study initiation: 3-6 g
ACCLIMATION
- Acclimation period: min. 12 d
- Acclimation conditions (same as test or not): 10-13°C in flow-through aquaria with dechlorinated tap water, O2 min. 6.4 mg/L, day/night 12/12 h
- Type and amount of food during acclimation: Tetramin flakes
- Feeding frequency during acclimation: daily
FEEDING DURING TEST
- Frequency: no feeding during test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 14°C
- pH:
- 8.0 - 8.2
- Dissolved oxygen:
- >4.8 mg/L
- Nominal and measured concentrations:
- Nominal: 100, 200, 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- No. of organisms per vessel: 8
- No. of vessels per concentration (replicates): 1
TEST SYSTEM
- Test vessel: aquaria
- Material, size, headspace, fill volume: glass, 36 x 23 x 26 cm (17 L)
- Aeration: yes
- No. of organisms per vessel: 8
- No. of vessels per concentration (replicates): 1
- No. of vessels per vehicle control (replicates): 1 (if applicable)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Culture medium different from test medium: yes
OTHER TEST CONDITIONS
- Adjustment of pH: yes (if not in range of 6 - 8.5)
- Photoperiod: 12/12 h
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality at 24, 48, 72 and 96 h. 72 and 96 h only observed if effects observed at earlier test stages. - Reference substance (positive control):
- yes
- Remarks:
- Fish are randomly tested for sensitivity 6 d after arrival from the source (test substance: HgCl2).
- Duration:
- 48 h
- Dose descriptor:
- LC0
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The 48-h LC50 to fish (Oncorhynchus mykiss) was determined to be >1000 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 21 - 23 April 1978
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Remarks:
- No information on negative control available.
- Qualifier:
- no guideline available
- Version / remarks:
- The test was conducted according to SOP A-11 (1977-09-01).
- Principles of method if other than guideline:
- - Principle of test: Fish are exposed to different solutions of the test substance.
- Short description of test conditions: 8-10 fish are used per concentration. Synthetic water is used, test concentrations are fixed.
- Parameters analysed / observed: Mortality (LC0, LC100), LC50 derivation is performed graphically and/or mathematically. - GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- not specified
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: dilution of a stock solution with synthetic water according to ISO norms
- Differential loading: 100, 200, 1000 mg/L
- Controls: positive control, solvent control if solvent used
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Methyl cellulose (2%) or ethanol (0.3%) or acetone (0.6%) if not water soluble - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout
- Source: P. Hohler, 4314 Zeiningen, Germany
- Age at study initiation: born in summer 1973
- Length at study initiation: 4-8 cm
- Weight at study initiation: 3-6 g
ACCLIMATION
- Acclimation period: min. 12 d
- Acclimation conditions (same as test or not): 10-13°C in flow-through aquaria with dechlorinated tap water, O2 min. 6.4 mg/L, day/night 12/12 h
- Type and amount of food during acclimation: Tetramin flakes
- Feeding frequency during acclimation: daily
FEEDING DURING TEST
- Frequency: no feeding during test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 14°C
- pH:
- 7.9 - 8.1
- Dissolved oxygen:
- >4.8 mg/L
- Nominal and measured concentrations:
- Nominal: 100, 200, 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- No. of organisms per vessel: 8
- No. of vessels per concentration (replicates): 1
TEST SYSTEM
- Test vessel: aquaria
- Material, size, headspace, fill volume: glass, 36 x 23 x 26 cm (17 L)
- Aeration: yes
- No. of organisms per vessel: 8
- No. of vessels per concentration (replicates): 1
- No. of vessels per vehicle control (replicates): 1 (if applicable)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Culture medium different from test medium: yes
OTHER TEST CONDITIONS
- Adjustment of pH: yes (if not in range of 6 - 8.5)
- Photoperiod: 12/12 h
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality at 24, 48, 72 and 96 h. 72 and 96 h only observed if effects observed at earlier test stages. - Reference substance (positive control):
- yes
- Remarks:
- Fish are randomly tested for sensitivity 6 d after arrival from the source (test substance: HgCl2).
- Duration:
- 48 h
- Dose descriptor:
- LC0
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
Not acutely toxic to fish with water from effluent (wastewater treatment plant) diluted by factor 5.
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The 48-h LC50 to fish (Oncorhynchus mykiss) was determined to be >1000 mg/L.
Referenceopen allclose all
Description of key information
Three 48-h LC50s were determined to be >1000 mg/L for Oncorhynchus mykiss. No acutely toxic effect to fish was observed up to the water solubility limit (< 1.0 mg/L) based on a weight-of-evidence approach.
Key value for chemical safety assessment
Additional information
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Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.