Tris(N-hydroxy-N-nitrosophenylaminato-O,O')aluminium

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April-July 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

not applicable

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

- Test item name: RCX 15-116
- Batch number: 15047AE2
- Other name(s): NPAL
- Appearance/colour: solid/whitish
- Chemical name: aluminium-N-nitrosophenylhydroxylamine (NPAL)
- CAS number: 15305-07-4
- Purity analysed: 99.1 %
- Molecular weight 438.3 g/mol
- Issue date of certificate: 08 Feb 2016
- Expiry date: 30 Nov 2016
- Stability: instable after repeated contact to air and / or light, undergoes hydrolysis
- Storage conditions: room temperature, protected from light

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

A microbial community serving as Inoculum at a concentration of 30 mg/L was obtained from a municipal sewage treatment plant treating predominantly domestic sewage.

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

During a 28 day period the O2 demand of the test item and several controls was evaluated in a climate controlled and closed system containing a CO2 absorbing agent, an electro-chemical oxygen generator and a switching manometer.
Treatment groups evaluated:
• Test item group (test item and inoculum)
• Procedure control group (reference item and inoculum)
• Inoculum control group (inoculum)
• Toxicity control group (test item, reference item and inoculum)

Reference substance:

benzoic acid, sodium salt

Remarks:

100 mg/L

Key result

Parameter:

% degradation (TOC removal)

Value:

2.8

Sampling time:

28 d

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

RCX 15-116 cannot be considered to be readily biodegradable according to OECD guideline 301 F.

Executive summary:

The ready biodegradability of RCX 15-116was assessed with the Manometric Respirometry Test according to OECD Guideline 301 F.RCX 15-116was tested at a nominal concentration of 100 mg/L.The following biodegradation was determined at the end of the 28-d period:

*        RCX 15-116 (100 mg/L):                   2.8 %

*        Sodium benzoate (100 mg/L):           92.7 %

Since the pass value of > 60 % was not reached within 28 days RCX 15-116 cannot be considered to be readily biodegradable according to OECD guideline 301 F. RCX 15-116 had no inhibitory effect on activated sludge microorganisms at the tested concentration of 100 mg/L.

Description of key information

The test substance is not readily biodegradable in the Manometric Respirometry Test (OECD 301 F).

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed