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EC number: 218-147-6 | CAS number: 2052-49-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Experimental data of test chemicals
- Justification for type of information:
- Data is from handbook or collection of data
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report was prepared based on two toxicity study of microorganism for the test chemical.
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Remarks:
- WoE 3
- Vehicle:
- yes
- Remarks:
- WoE 3
- Test organisms (species):
- other: WoE 2: Vibrio fisheri and Perenniporia tephropora was used in WoE 3 study
- Details on inoculum:
- WoE 2: - Common name: Aliivibrio fischeri
WoE 3: Perenniporia tephropora is a wood decay fungi - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 15 min
- Remarks on exposure duration:
- 21 days exposure were provided in WoE 3 study
- Post exposure observation period:
- WoE 2: Light emission of the bacteria was measured after 0, 5 and 15 min of exposure using a Microtoxs Model 500 Toxicity Analyzer.
- Test temperature:
- WoE 3: 25°C
- Nominal and measured concentrations:
- WoE 2: Nine concentrations were tested in a 1 : 2 dilution series
- Details on test conditions:
- WoE 2:
- No. of vessels per concentration (replicates): triplicates
WoE 3:
- No. of vessels per concentration (replicates): quadruplicate - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 15 min
- Dose descriptor:
- EC50
- Effect conc.:
- 284 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: WoE 2
- Duration:
- 21 d
- Dose descriptor:
- other: MIC
- Effect conc.:
- 4.2 other: μmol/cm2
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks:
- radial growth
- Remarks on result:
- other: WoE 3
- Reported statistics and error estimates:
- WoE 2: The differences tested by ANOVA are highly significant (P < 0.001)
- Validity criteria fulfilled:
- not specified
- Conclusions:
- As in the second weight of evidence study after the exposure of Vibrio fischeri with a test chemical for 15 min, bioluminescence inhibition of microorganism was observed and the EC50 was determined to be at 284 mg/l with 95% confidence limit of 241–336 mg/l, whereas in the third study minimum inhibition concentration (MIC) was determined to be > 4.2 umol/cm2. Thus based on the above all studies, it is observed that the test chemical was non toxic.
- Executive summary:
Data available for the test chemicals has been reviewed to determine the toxicityof the test chemical on microorganisms. The studies are as mentioned below:
Toxicity to V. fischeri was measured as inhibition of bioluminescence using Microtoxs M500 Rapid Toxicity Testing System equipment and consumables.The assay was carried out as described in the Microtox user’s manual. Nine concentrations were tested in a 1 : 2 dilution series. Test conducted in triplicates. Light emission of the bacteria was measured after 0, 5 and 15 min of exposure using a Microtoxs Model 500 Toxicity Analyzer. The differences tested by ANOVA are highly significant (P < 0.001). After the exposure of Vibrio fischeri with a test chemical for 15 min, bioluminescence inhibition of microorganism was observed and the EC50 was determined to be at 284 mg/l with 95% confidence limit of 241–336 mg/l.
Above study further supported by the study from peer reviewed journal. Principle of this study was to determine the effect of test chemical on the Perenniporia tephropora. Test chemical analytically monitorized by NMRspectroscopy, ES-MS and X-ray crystallography. The toxicity test, cellulose papers i.e. Whatman No. 41, diameter 5.5 cm, thickness 510μm were treated with solutions of the compounds in acetonitrile or water (0.8–8.4μmol cm−2) and allowed to air-dry. Solvent controls were also prepared in the same manner but without addition of the test compound. The cellulose papers were sterilized by gamma irradiation (25 kGy). Under the sterile conditions, the cellulose papers loaded with test chemical were placed in center on 90 mm malt agar plates and inoculated with a small plug (7mm × 5 mm) of the test fungi Perenniporia tephropora. The fungi were sub-cultured on malt agar plates from existing stocks and used within about 7–9 days. After inoculation process, the plates were incubated at 25°C and at 29% relative humidity for three weeks. Fungal growth was scored periodically by measuring its growth diameters. Test was performed in quadruplicate. After the exposure of test chemical with the fungi, chemical did not inhibit fungi (Perenniporia tephropora) growth under bioassay conditions and minimum inhibition concentration (MIC) was determined to be > 4.2 umol/cm2.
Thus based on the above all studies, it is observed that the test chemical was non toxic.
Reference
Description of key information
As in the second weight of evidence study after the exposure of Vibrio fischeri with a test chemical for 15 min, bioluminescence inhibition of microorganism was observed and the EC50 was determined to be at 284 mg/l with 95% confidence limit of 241–336 mg/l, whereas in the third study minimum inhibition concentration (MIC) was determined to be > 4.2 umol/cm2. Thus based on the above all studies, it is observed that the test chemical was non toxic.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 284 mg/L
Additional information
Data available for the test chemicals has been reviewed to determine the toxicityof the test chemical on microorganisms. The studies are as mentioned below:
Toxicity to V. fischeri was measured as inhibition of bioluminescence using Microtoxs M500 Rapid Toxicity Testing System equipment and consumables.The assay was carried out as described in the Microtox user’s manual. Nine concentrations were tested in a 1 : 2 dilution series. Test conducted in triplicates. Light emission of the bacteria was measured after 0, 5 and 15 min of exposure using a Microtoxs Model 500 Toxicity Analyzer. The differences tested by ANOVA are highly significant (P < 0.001). After the exposure of Vibrio fischeri with a test chemical for 15 min, bioluminescence inhibition of microorganism was observed and the EC50 was determined to be at 284 mg/l with 95% confidence limit of 241–336 mg/l.
Above study further supported by the study from peer reviewed journal. Principle of this study was to determine the effect of test chemical on the Perenniporia tephropora. Test chemical analytically monitorized by NMRspectroscopy, ES-MS and X-ray crystallography. The toxicity test, cellulose papers i.e. Whatman No. 41, diameter 5.5 cm, thickness 510μm were treated with solutions of the compounds in acetonitrile or water (0.8–8.4μmol cm−2) and allowed to air-dry. Solvent controls were also prepared in the same manner but without addition of the test compound. The cellulose papers were sterilized by gamma irradiation (25 kGy). Under the sterile conditions, the cellulose papers loaded with test chemical were placed in center on 90 mm malt agar plates and inoculated with a small plug (7mm × 5 mm) of the test fungi Perenniporia tephropora. The fungi were sub-cultured on malt agar plates from existing stocks and used within about 7–9 days. After inoculation process, the plates were incubated at 25°C and at 29% relative humidity for three weeks. Fungal growth was scored periodically by measuring its growth diameters. Test was performed in quadruplicate. After the exposure of test chemical with the fungi, chemical did not inhibit fungi (Perenniporia tephropora) growth under bioassay conditions and minimum inhibition concentration (MIC) was determined to be > 4.2 umol/cm2.
Thus based on the above all studies, it is observed that the test chemical was non toxic.
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