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EC number: 944-073-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- published in O.J. L 142 (2008)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Test substance name: Acid Brown 235
- Other name: Korostan Brown DGR
- Source and lot/batch No. of test material: 7012/2007
- Expiration date of the lot/batch: May 2018
- Appearance: dark brown powder - Analytical monitoring:
- yes
- Details on sampling:
- The analytical determination of the test substance concentrations was performed at the beginning and at the end of the test. The samples for analysis were taken from the highest (100 mg/L) and the lowest test concentration (10 mg/L). The samples for analysis (0 hours) were prepared at the beginning of the test and immediately delivered in transport box to analytical laboratory. An analysis of samples was started immediately after delivery. The samples for analysis at the end of the test (48 hours) were delivered to analytical laboratory immediately after the end of testing. An analysis of samples was started immediately after delivery. All samples were stored at laboratory temperature.
The analytical results showed that the test substance was stable in dilution water under current test conditions. - Vehicle:
- no
- Details on test solutions:
- For holding and culturing of the daphnia the M4 medium was used (according to Annex 2 of the EU method C.2 and according to Annex 3 of the OECD TG 202).
For test was used dilution water (according to Annex 2 of the EU method C.2 and according to Annex 3 of the OECD TG 202). Dilution water was prepared just before the test by admixing 2.5 mL of stored solutions A, B, C and D into 1 000 mL of deionized water.
A: 117.6 g CaCl2·2H20 in 1L deionized water
B: 49.3 g MgSO4·7H2O in 1L deionized water
C: 25.9 g NaHCO3 in 1L deionized water
D: 2.3 g KCl in 1L deionized water
The dilution water should have a pH 7.8 ± 0.2, values measured in this study were 8.0 in the preliminary test.
The content of sum Ca + Mg ions should have the value of 2.55 ± 0.10 mmol/L, values measured in this study were 2.55 mmol/L in the preliminary test.
Measured values meet the requirements. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Straus
- Source: obtained by aperiodic parthenogenesis from own laboratory breeding
- Feeding during test: no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 20±2°C, with a variation of ± 1°C at each individual test
- pH:
- 7.6 - 8.0
- Dissolved oxygen:
- 8.2 - 8.6 mg/L
- Nominal and measured concentrations:
- Nominal concentration: 10 mg/L of the test substance
8.33 mg/L (0 h)
8.29 mg/L (48 h)
Nominal concentration: 100 mg/L of the test substance
96.6 mg/L (0 h)
102.1 mg/L (48 h) - Details on test conditions:
- TEST SYSTEM
- Test vessel: laboratory beakers
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: daylight - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (CAS: 7778-50-9)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- see Table 1
- Results with reference substance (positive control):
- RESULTS WITH REFERENCE SUBSTANCE
- Identification of the reference substance: potassium dichromate (CAS: 7778-50-9)
- EC50 = 0.62 mg/L (48 h, Daphnia magna)
- Interlaboratory test data: EC50 = 0.46 - 1.08 mg/L (48 h, Daphnia magna) - Validity criteria fulfilled:
- yes
- Conclusions:
- The experiment with the concentration of 100 mg/L included in the preliminary test has been performed using 20 daphnia, therethrough the conditions of the limit test given in guidelines have been fulfilled. It was demonstrated that the EC50 is higher than this concentration.
No immobilisation was observed in the preliminary test. Therefore exact values of EC50 could not be calculated and the values of EC are given in the form of a range. The EC0 and EC100 values are identified by direct observation.
The analytical results from the preliminary test showed, that the test substance, Acid Brown 235, was stable in the dilution water at conditions of the test.
There is stated in the guideline that, if evidence is available to demonstrate that the concentration of the test substance has been satisfactorily maintained within ± 20 percent of the nominal or measured initial concentration throughout the test, the results can be based on nominal or measured initial values. The nominal concentrations were used for all evaluation and results.
Test results:
EC50 > 100 mg/L (Daphnia magna, 24 h) (nominal concentration)
EC50 > 100 mg/L (Daphnia magna, 48 h) (nominal concentration)
EC0 = 100 mg/L (Daphnia magna, 24 h) (nominal concentration)
EC0 = 100 mg/L (Daphnia magna, 48 h) (nominal concentration)
EC100 > 100 mg/L (Daphnia magna, 24 h) (nominal concentration)
EC100 > 100 mg/L (Daphnia magna, 48 h) (nominal concentration) - Executive summary:
The test substance, Acid Brown 235, was tested in acute immobilisation test on Daphnia magna. The test was performed according to method C.2 – Daphnia sp. Acute Immobilisation Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
The preliminary test was performed in a range of the test substance nominal concentrations 10 – 100 mg/L. The test was performed as static. Based on no toxicity of the test substance found in preliminary test, the study was terminated. The experiment with the concentration of 100 mg/L included in the preliminary test has been performed using 20 daphnia, therethrough the conditions of the limit test given in guideline have been fulfilled. It was demonstrated that the EC50 is higher than this concentration.
The samples for analytical determination were taken at the beginning and at the end of the test. The analytical results from the preliminary test showed, that the test substance, Acid Brown 235, was stable in the dilution water at conditions of the test.
There is stated in the guideline that, if evidence is available to demonstrate that the concentration of the test substance has been satisfactorily maintained within ± 20 percent of the nominal or measured initial concentration throughout the test, the results can be based on nominal or measured initial values.
The nominal concentrations were used for all evaluation and results.
Test results:
EC50 > 100 mg/L (Daphnia magna, 24 h) (nominal concentration)
EC50 > 100 mg/L (Daphnia magna, 48 h) (nominal concentration)
EC0 = 100 mg/L (Daphnia magna, 24 h) (nominal concentration)
EC0 = 100 mg/L (Daphnia magna, 48 h) (nominal concentration)
EC100 > 100 mg/L (Daphnia magna, 24 h) (nominal concentration)
EC100 > 100 mg/L (Daphnia magna, 48 h) (nominal concentration)
Reference
Table 1: Immobilisation of daphnia
Nominal concentration |
mg/L |
100 |
80 |
40 |
20 |
10 |
Control |
Number |
24 hours 1stseries |
10 |
10 |
10 |
10 |
10 |
10 |
Number |
24 hours 2ndseries |
10 |
10 |
10 |
10 |
10 |
10 |
Number |
24 hours total |
20 |
20 |
20 |
20 |
20 |
20 |
Immobilisation % |
24 hours |
0 |
0 |
0 |
0 |
0 |
0 |
Number |
48 hours 1stseries |
10 |
10 |
10 |
10 |
10 |
10 |
Number |
48 hours 2ndseries |
10 |
10 |
10 |
10 |
10 |
10 |
Number |
48 hours total |
20 |
20 |
20 |
20 |
20 |
20 |
Immobilisation % |
48 hours |
0 |
0 |
0 |
0 |
0 |
0 |
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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