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EC number: 219-514-3 | CAS number: 2451-62-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Repeated dose toxicity: inhalation
Administrative data
- Endpoint:
- short-term repeated dose toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: all relevant data available
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 412 (Subacute Inhalation Toxicity: 28-Day Study)
- Deviations:
- yes
- Remarks:
- similar to subchronic inhalation study but designed as RF-study
- Principles of method if other than guideline:
- similar to subchronic inhalation study but designed as RF-study
- GLP compliance:
- yes
- Limit test:
- no
Test material
- Reference substance name:
- 1,3,5-tris(oxiranylmethyl)-1,3,5-triazine-2,4,6(1H,3H,5H)-trione
- EC Number:
- 219-514-3
- EC Name:
- 1,3,5-tris(oxiranylmethyl)-1,3,5-triazine-2,4,6(1H,3H,5H)-trione
- Cas Number:
- 2451-62-9
- Molecular formula:
- C12H15N3O6
- IUPAC Name:
- tris[(oxiran-2-yl)methyl]-1,3,5-triazinane-2,4,6-trione
- Details on test material:
- TGIC (technical grade, batch-no. 177108)
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- CD-1
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- exact strain designation: CD-1(ICR)BR strain
Mice were housed two per cage in stainless-steel wire-mesh cages at 18.9 – 22.2 °C, a relative humidity of 47 - 61 %, and at a 12-hour datk/light cycle. Food and water was provided ad libitum. Individual numbering was by tail tattoo.
Acclimatization period was at least 5 days.
Administration / exposure
- Route of administration:
- inhalation: dust
- Type of inhalation exposure:
- whole body
- Vehicle:
- other: unchanged (no vehicle)
- Details on inhalation exposure:
- 4 groups of 12 male mice were exposed to TGIC at 0. 100, 350 and 750 mg/m3 air, 6 hours/day, for 5 consecutive days (whole-body exposure), followed by a 14-day recovery period for further observations.
The volume of the inhalation chamber was 1330 l, and the mouse inhalation cages were placed inside the chamber; air flow was 300 l/min, and dust was generated by an Auger Dust Feeder (Spring Tool Co., Schoolcraft, MI, USA), and concentrations (gravimetric on fiber filters) as well as particle size (using a cascade impactor) were measured several times during each daily exposure. - Analytical verification of doses or concentrations:
- no
- Duration of treatment / exposure:
- 6 hours / day, Whole body exposure
- Frequency of treatment:
- on 5 consecutive days
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0, 100, 350, 750 mg/m3 air
Basis:
nominal conc.
- No. of animals per sex per dose:
- 12 male mice / group
- Control animals:
- yes, sham-exposed
- Details on study design:
- Body weights were measured shortly before the first exposure, and shortly after the last exposure, and on days 7 and 14 post exposure. Gross necropsy was performed after sacrifice on all organs , and gross lesions were preserved in 10% neutral formalin. All parameters measured were statistically analysed with appropriate methods.
- Positive control:
- none
Examinations
- Observations and examinations performed and frequency:
- Body weights were measured shortly before the first exposure, and shortly after the last exposure, and on days 7 and 14 post exposure.
- Sacrifice and pathology:
- Necropsy was performed after sacrifice on all organs , and gross lesions were preserved in 10% neutral formalin. All parameters measured were statistically analysed with appropriate methods.
- Statistics:
- yes
Results and discussion
Results of examinations
- Clinical signs:
- effects observed, treatment-related
- Mortality:
- mortality observed, treatment-related
- Body weight and weight changes:
- effects observed, treatment-related
- Food consumption and compound intake (if feeding study):
- not examined
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- not examined
- Ophthalmological findings:
- not examined
- Haematological findings:
- not examined
- Clinical biochemistry findings:
- not examined
- Urinalysis findings:
- not examined
- Behaviour (functional findings):
- no effects observed
- Organ weight findings including organ / body weight ratios:
- effects observed, treatment-related
- Gross pathological findings:
- effects observed, treatment-related
- Histopathological findings: non-neoplastic:
- not examined
- Histopathological findings: neoplastic:
- not examined
- Details on results:
- Mortality (or sacrifice in moribund condition) was observed in all three dose groups:
at 100 mg/m3 5/12 (days 5-9),
at 350 mg/m3 12/12 (days 3-11), and
at 750 mg/m3 11/12 (days 2-4).
Clinical signs were found in all dose groups and included unkemptness, audible respiration, gasping, blepharospasm, swollen periocular tissue, and dehydration, and perioral wetness was observed at 350 and 750 mg/m3 air.
Body weights were reduced in all dose groups in a concentration-related manner, even during the recovery period additional weight loss was observed at 100 mg/m3 air.
At necropsy gas accumulation in all TGIC-treated animals was found in the gastro-intestinal tract, hyperinflation of the lungs at 350 and 750 mg/m3 air, decreased splee sizein all groups. Also treatment-related were crusts on the skin, perioral areas, perinasal area, and periocular aerea as well as color changes in the lungs.
Effect levels
- Dose descriptor:
- NOAEL
- Effect level:
- <= 100 mg/m³ air (nominal)
- Sex:
- male
- Basis for effect level:
- other: mortality
- Remarks on result:
- not determinable
- Remarks:
- no NOAEL identified
Target system / organ toxicity
- Critical effects observed:
- not specified
Any other information on results incl. tables
No NOAEL or NOEL was determined
Applicant's summary and conclusion
- Conclusions:
- 6-hour inhalation exposure for 5 consecutive days caused mortality among the male mice exposued
at 100 mg/m3 5/12 (days 5-9),
at 350 mg/m3 12/12 (days 3-11), and
at 750 mg/m3 11/12 (days 2-4).
In addition clinical signs and reduced body weights were observed in a dose-related manner.
No NOAEL or NOEL was determined
The NOEL for this study is below 100 mg/m3 air of TGIC - Executive summary:
6-hour inhalation exposure for 5 consecutive days caused mortality among the male mice exposued at 100 mg/m3 5/12 (days 5-9), at 350 mg/m3 12/12 (days 3-11), and at 750 mg/m3 11/12 (days 2-4). In addition clinical signs and reduced body weights were observed in a dose-related manner. No NOAEL or NOEL was determined The NOEL for this study is below 100 mg/m3 air of TGIC
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