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EC number: 274-700-1 | CAS number: 70616-89-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- published in O.J. L 142 (2008)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- The analytical determination of the test substance concentrations were performed at the beginning of the test and at the end of the test. The samples for analysis were taken from the highest (100 mg/L) and the lowest test concentration (1 mg/L). The samples for analysis (0 hours) were prepared at the beginning of the test and immediately delivered in transport box to analytical laboratory. The samples were analyzed on the day of delivery. The samples for analysis at the end of the test (96 hours) were delivered to analytical laboratory immediately after the end of testing. The samples were analyzed on the day of delivery. All samples were stored at laboratory temperature.The analytical results showed that the test substance was stable in dilution water under current test conditions.
- Vehicle:
- no
- Details on test solutions:
- Standard dilution water was prepared just before the test by admixing 2.5 mL of stored solutions A, B, C and D in 1000 mL of deionized water.A: 117.6 g CaCl2·2H20 in 1L deionized water (Batch No.: PP/2015/01588)B: 49.3 g MgSO4·7H2O in 1L deionized water (Batch No.: PP/2014/03001)C: 25.9 g NaHCO3 in 1L deionized water (Batch No.: PP/2016/04662)D: 2.3 g KCl in 1L deionized water (Batch No.: PP/2015/02174)The dilution water should have a pH 7.8±0.2, value measured in this study was 8.0 in the preliminary test. The content of sum Ca + Mg ions should have the value of 2.50±0.10 mmol/L, value measured in this study was 2.48 mmol/L in the preliminary test. The stock solution of the test substance was prepared in the dilution water. 200 mg of the test substance was weighed into 2000 mL of the dilution water for the preliminary test. The concentrations of solutions used in the preliminary test were obtained by dilution of the stock solution with dilution water.
- Test organisms (species):
- Poecilia reticulata
- Details on test organisms:
- TEST ORGANISM- Common name: Poecilia reticulata- Source: certified supplier EMPLA s.r.o., Hradec Králové (Czech Republic) - Age at study initiation: 3 - 4 months- Length at study initiation: 2 - 3 cmACCLIMATION- Acclimation period: 7 days- Acclimation conditions: in dilution water under same conditions as in the test- Type and amount of food during acclimation: “TetraMin” – complete feed for aquarium fish (manufacturer: Tetra, Germany), - Feeding frequency during acclimation: once a day- Health during acclimation (any mortality observed): The mortality of the fish was observed for 12 days from the times of delivery. The recorded mortality was 0.33 % (1 fish from 300 individuals).FEEDING DURING TEST- without feeding, without aeration
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Test temperature:
- 21.1 - 21.8°C
- pH:
- 7.3 - 8.0
- Dissolved oxygen:
- 6.8 - 8.3 mg/L
- Conductivity:
- 2.48 mmol/L Ca+Mg
- Nominal and measured concentrations:
- Nominal concentration: 100 mg/L 0 h: 99.7 mg/L (measured) 96 h: 106.2 mg/L (measured)Nominal concentration: 1 mg/L 0 h: 0.98 mg/L (measured) 96 h: 1.03 mg/L (measured)
- Details on test conditions:
- TEST SYSTEM - Test vessel: glass beaker - Aeration: no - No. of organisms per vessel: 7 - No. of vessels per concentration (replicates): 1 - No. of vessels per control (replicates): 1 OTHER TEST CONDITIONS - Adjustment of pH: no - Photoperiod: daylight TEST CONCENTRATIONS - Test concentrations: 100, 50, 10, 5 and 1 mg/L
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- see Table 1
- Results with reference substance (positive control):
- The sensitivity of the test species and correctness of test performance is periodically verified in six-month period by testing the reference substance, potassium dichromate.The results of verification test with K2Cr2O7 are the following:LC50 = 152 mg/L (96 h)From the result of the last interlaboratory test arranged by CSlab spol. s r.o. in 2016 we have obtained data for potassium dichromate 96 hours – LC50 generated at 12 laboratories. The following reference range of values could be calculated from those data:LC50 = 86 – 200 mg/L (96 h)The mortality of test organisms by the reference substance (96 hours, LC50) obtained from our last reference test meets the calculated range from the interaboratory test.
- Sublethal observations / clinical signs:
Table 1: Fish mortality
Nominal concentration
mg/L
100
50
10
5
1
Control
Number of fish alive
2 hours
7
7
7
7
7
7
Mortality %
2 hours
0
0
0
0
0
0
Number of fish alive
24 hours
7
7
7
7
7
7
Mortality %
24 hours
0
0
0
0
0
0
Number of fish alive
48 hours
7
7
7
7
7
7
Mortality %
48 hours
0
0
0
0
0
0
Number of fish alive
72 hours
7
7
7
7
7
7
Mortality %
72 hours
0
0
0
0
0
0
Number of fish alive
96 hours
7
7
7
7
7
7
Mortality %
96 hours
0
0
0
0
0
0
- Validity criteria fulfilled:
- yes
- Conclusions:
- The experiment with the concentration of 100 mg/L included in the preliminary test has been performed using 7 fish, therethrough the conditions of the limit test have been fulfilled. It was demonstrated that the LC50 is higher than this concentration.No mortality was observed in the preliminary test. The exact values of LC50 could not be calculated and the values of LC are given in the form of a range. The LC100 and LC0 values were identified by direct observation.There is stated in the guideline that, if evidence is available to demonstrate that the concentration of the test substance has been satisfactorily maintained within ± 20 percent of the nominal or measured initial concentration throughout the test, the results can be based on nominal or measured initial values. We choose the nominal concentrations for the evaluation.LC50 > 100 mg/L (96 h) (nominal concentration)LC50 > 100 mg/L (72 h) (nominal concentration)LC50 > 100 mg/L (48 h) (nominal concentration)LC50 > 100 mg/L (24 h) (nominal concentration)LC0 = 100 mg/L (96 h) (nominal concentration)LC0 = 100 mg/L (72 h) (nominal concentration)LC0 = 100 mg/L (48 h) (nominal concentration)LC0 = 100 mg/L (24 h) (nominal concentration)LC100 > 100 mg/L (96 h) (nominal concentration)LC100 > 100 mg/L (72 h) (nominal concentration)LC100 > 100 mg/L (48 h) (nominal concentration)LC100 > 100 mg/L (24 h) (nominal concentration)
- Executive summary:
The test substance, Reactive Orange 13, was tested for acute toxicity on fish Poecilia reticulata. The test was performed according to method C.1 – Acute toxicity for fish, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
The preliminary test was performed in a range of the test substance nominal concentrations 1 – 100 mg/L. Based on no toxicity of the test substance found in the preliminary test, the study was terminated. The experiment with the concentration of 100 mg/L included in the preliminary test has been performed using 7 fish, therethrough the conditions of the limit test have been fulfilled. It was demonstrated that the LC50is higher than this concentration. The test was performed as static.
Samples for the analytical determination were taken at the beginning and at the end of the test. The analytical results from the preliminary test showed, that the test substance was stable in dilution water at conditions of the test.
There is stated in the guideline that, if evidence is available to demonstrate that the concentration of the test substance has been satisfactorily maintained within ± 20 percent of the nominal or measured initial concentration throughout the test, the results can be based on nominal or measured initial values.
For all evaluations and results the nominal concentrations of the test substance were used.
LC50> 100 mg/L (96 h) (nominal concentration)
LC50> 100 mg/L (72 h) (nominal concentration)
LC50> 100 mg/L (48 h) (nominal concentration)
LC50> 100 mg/L (24 h) (nominal concentration)
LC0= 100 mg/L (96 h) (nominal concentration)
LC0= 100 mg/L (72 h) (nominal concentration)
LC0= 100 mg/L (48 h) (nominal concentration)
LC0= 100 mg/L (24 h) (nominal concentration)
LC100> 100 mg/L (96 h) (nominal concentration)
LC100> 100 mg/L (72 h) (nominal concentration)
LC100> 100 mg/L (48 h) (nominal concentration)
LC100> 100 mg/L (24 h) (nominal concentration)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The study report contains the limited information on the test conditions because the study has been performed in 1994. However available information is sufficient to conclude on the classification of the substance. Further the test was performed on the vertebrates and use of results from old experimental studies is one of the options to provide information requested by REACH. New experimental studies with vertebrates must only be conducted if there is no adequate existing information.
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- no
- Test organisms (species):
- Poecilia reticulata
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- The exposure time is not explicitly reported. In this laboratory, the exposure time for an acute toxicity test on fish was 96 h.
- Nominal and measured concentrations:
- 1000, 900, 800 and 600 mg/l
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- No death was observed
- Conclusions:
- According to Regulation (EC) No. 1272/2008 (CLP), the test substance, Reactive Orange 13, need not be classified as Aquatic Acute substance.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Justification for type of information:
- None
- Principles of method if other than guideline:
- None
- GLP compliance:
- no
- Specific details on test material used for the study:
- None
- Analytical monitoring:
- not specified
- Details on sampling:
- None
- Vehicle:
- yes
- Remarks:
- water
- Details on test solutions:
- None
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- None
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- None
- Post exposure observation period:
- None
- Hardness:
- None
- Test temperature:
- 25
- pH:
- 10
- Dissolved oxygen:
- None
- Salinity:
- None
- Conductivity:
- None
- Nominal and measured concentrations:
- 300 and 1000 mg/l
- Details on test conditions:
- None
- Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- None
- Results with reference substance (positive control):
- None
- Reported statistics and error estimates:
- None
- Sublethal observations / clinical signs:
None
- Validity criteria fulfilled:
- no
- Conclusions:
- The LC50 of FAT 40170 in this fish acute toxicity test was determined to be greater than 1000 mg/L.
- Executive summary:
An acute fish toxicity test was performed with Rainbow trout species. Groups of fish were exposed at the test concentrations of 300.0 and 1000.0 mg/L. No mortality was observed throughout the duration of the test. Hence, the LC50 of FAT 40170 was determined to be >1000 mg/L.
Referenceopen allclose all
Description of key information
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.