Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 248-053-0 | CAS number: 26850-47-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1982
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2-[[4-[ethyl(2-hydroxyethyl)amino]phenyl]azo]-6-methoxy-3-methylbenzothiazolium chloride
- EC Number:
- 248-053-0
- EC Name:
- 2-[[4-[ethyl(2-hydroxyethyl)amino]phenyl]azo]-6-methoxy-3-methylbenzothiazolium chloride
- Cas Number:
- 26850-47-5
- Molecular formula:
- C19H23ClN4O2S
- IUPAC Name:
- 2-[(E)-2-{4-[ethyl(2-hydroxyethyl)amino]phenyl}diazen-1-yl]-6-methoxy-3-methyl-1,3-benzothiazol-3-ium chloride
- Test material form:
- solid
- Details on test material:
- see below
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Tif:RAIf(SPF)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: CIBA-GEIGY LTD. Tierfarm, 4334 Sisseln, Switzerland
- Females (if applicable) nulliparous and non-pregnant: [yes]
- Age at study initiation: 7-8 weeks
- Weight at study initiation: 157-190 g
- Fasting period before study:
- Housing: 5/cage/sex
- Diet (e.g. ad libitum): NAFAG No. 890, NAFAG AG, Gossau, SG (Switzerland) ad libitum
- Water (e.g. ad libitum): tap water ad libitum
- Acclimation period:
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 3
- Humidity (%): 55 +/- 15
- Air changes (per hr): ca. 15
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: From: March 3, 1982 To: March 29, 1982
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- polyethylene glycol
- Remarks:
- 400
- Details on oral exposure:
- VEHICLE
- Amount of vehicle (if gavage): 10 and 20 mL/kg
MAXIMUM DOSE VOLUME APPLIED: 20 mL/kg - Doses:
- 300, 1000, 2500, 5000 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing:
Mortality: daily, a.m. and p.m. on working days
Signs and Symptoms: daily
Body weight: on days 1, 7, 14 and at death
- Necropsy of survivors performed: yes
Necropsies: Spontaneously dying animals were submitted to a gross necropsy as soon as possible; survivors at the end of the observation period.
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other: - Statistics:
- From the body weights, the group means and their standard deviations were calculated.
The LD50 including the 95% confidence limit were computed by the logit method (J. Berkson, J.Am. Stat. Ass. 39.357-65, 1944)
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 2 004 mg/kg bw
- Based on:
- test mat.
- Remarks:
- API: 55.6%
- 95% CL:
- >= 966 - <= 7 827
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 2 152 mg/kg bw
- Based on:
- test mat.
- Remarks:
- API: 55.6%
- 95% CL:
- >= 660 - <= 82 457
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 2 120 mg/kg bw
- Based on:
- test mat.
- Remarks:
- API: 55.6%
- 95% CL:
- >= 1 279 - <= 4 373
- Mortality:
- No deaths occurred at 300 mg/kg bw.
At 1000 mg/kg bw, 1 male and 2 females died within two days.
At 2500 mg/kg bw, 2 males and 2 females died within two days.
At 5000 mg/kg bw, all males and 3 females died on Day 1; one further female died on Day 13. - Clinical signs:
- Only signs of unspecific toxicity were observed at all dose levels, such as, sedation, dyspnoea, exaphthalmos, ruffled fur, curved body position
- Body weight:
- All surviving animals showed regular body weight development
- Gross pathology:
- No compound related gross organ changes were observed.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Remarks:
- calculated for 100% API
- Executive summary:
Upon a acute oral administration and a 14 day post-treatment observation period, the following lethal doses (with 95 % confidence limits) were
determined for Basic Blue 41 Cl (purity 55.6%).
LD50 in male rats: 2004 (966-7827) mg/kg bw.
LD50 in female rats: 2152 (660-82457) mg/kg bw.
LD50 in rats of both sexes: 2120(1279-4373) mg/kg bw.
Signs of unspecitic toxicity were seen at all dose levels.
According to the company standard Basic Blue 41 has a slight acute toxicity when administered orally to the albino rat.
Calculated for 100% API, the LD50 lies between 300 and 2000 mg/kg bw, and hence results in classification in Category 4 based on GHS CLP criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)