Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 241-409-6 | CAS number: 17372-87-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed according to the guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Principles of method if other than guideline:
- Acute Immobilization Study of test material on Daphnia magna in a Semi-Static System for 48 hrs. Test performed according to the OECD guideline 202.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media having concentration 1g/L. Achieving test concentrations of 6.25 mg/L,12.5 mg/L,25 mg/L, 50 mg/L and 100 mg/L, respectively.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- 24 and 48 hrs
- Hardness:
- 150.5 mg of CaCO3
- Test temperature:
- 20±2
- pH:
- 7.4
- Nominal and measured concentrations:
- 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids
OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark
- Reference substance (positive control):
- not specified
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: No immobility was observed
- Validity criteria fulfilled:
- yes
- Remarks:
- 1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels
- Conclusions:
- Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test material on test daphnia was found to be > 100 mg/L.
- Executive summary:
Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test material. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media having concentration 1g/L. Achieving test concentrations of 6.25 mg/L,12.5 mg/L,25 mg/L, 50 mg/L and 100 mg/L, respectively.
The nominal concentration selected for the experiment were 6.25 mg/L,12.5 mg/L,25 mg/L, 50 mg/L and 100 mg/L and test Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC50) for test material on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be >100 mg/l.Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the test substance, does not exhibit short term toxicity to Daphnia.After 48 hours of exposure to test item to various nominal test concentrations, EC50 was determine to be >100 mg/l . Based on the EC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.
Reference
9.1. Immobility
Nominal |
Number |
of |
Immobility |
|
|
Concentration |
Daphnids |
0 h |
24 h |
|
48 h |
(mg/l) |
|
|
|
|
|
Control |
10 |
- |
- |
|
- |
6.25 |
10 |
- |
- |
|
- |
12.5 |
10 |
- |
- |
|
- |
25 |
10 |
- |
- |
|
- |
50 |
10 |
- |
- |
|
- |
100 |
10 |
- |
- |
|
- |
9.2. pH and oxygen content:
Nominal Concentration Number of |
pH(mg/L) |
|
||
(mg/l) |
|
daphnids |
0h |
48h |
Control |
|
10 |
7.2 |
7.3 |
6.25 |
|
10 |
7.2 |
7.3 |
12.5 |
|
10 |
7.3 |
7.4 |
25 |
|
10 |
7.4 |
7.5 |
50 |
|
10 |
7.4 |
7.5 |
100 |
|
10 |
7.5 |
7.6 |
Nominal Concentration Number of |
DO(mg/L) |
|
||
(mg/l) |
|
daphnids |
0h |
48h |
Control |
|
10 |
7.1 |
6.9 |
6.25 |
|
10 |
7.1 |
6.9 |
12.5 |
|
10 |
7.1 |
6.9 |
25 |
|
10 |
7.1 |
6.9 |
50 |
|
10 |
7.1 |
6.9 |
100 |
|
10 |
7.1 |
6.9 |
9.3. Test temperature:
Nominal Concentration Number of |
Temparature |
|||||
|
daphnids |
0h |
48h |
|
||
|
10 |
20.1 |
20.1 |
|
||
|
10 |
20.1 |
20.1 |
|
||
|
10 |
20.1 |
20.1 |
|
||
|
10 |
20.1 |
20.1 |
|
||
|
10 |
20.1 |
20.1 |
|
||
|
10 |
20.1 |
20.1 |
|
Description of key information
Short term toxicity to aquatic invertebrate:
Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test material. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media having concentration 1g/L. Achieving test concentrations of 6.25 mg/L,12.5 mg/L,25 mg/L, 50 mg/L and 100 mg/L, respectively.
The nominal concentration selected for the experiment were 6.25 mg/L,12.5 mg/L,25 mg/L, 50 mg/L and 100 mg/L and test Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC50) for test material on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be >100 mg/l.Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the test substance, does not exhibit short term toxicity to Daphnia.After 48 hours of exposure to test item to various nominal test concentrations, EC50 was determine to be >100 mg/l . Based on the EC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Short term toxicity to aquatic invertebrate:
The short term toxicity to aquatic invertebrate for the test material was determined based on the data derived from two experimental reports .
In one report , Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test material. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media having concentration 1g/L. Achieving test concentrations of 6.25 mg/L,12.5 mg/L,25 mg/L, 50 mg/L and 100 mg/L, respectively.
The nominal concentration selected for the experiment were 6.25 mg/L,12.5 mg/L,25 mg/L, 50 mg/L and 100 mg/L and test Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC50) for test material on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be >100 mg/l.Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the test substance, does not exhibit short term toxicity to Daphnia.After 48 hours of exposure to test item to various nominal test concentrations, EC50 was determine to be >100 mg/l . Based on the EC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.
In another report Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.
The stock solution 100.0 mg/l was prepared by dissolving dark red powder in reconstituted water. The test solutions of required comcentrations were prepared by mixing the stock solution of the test sample in reconstituted water.12 , 20 , 32 , 50 , 80 , 100 mg/lconcentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.
The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 51.9 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate and can be classified as aquatic chronic 3 category as per the CLP classification criteria.
The effect concentration determined by both the study reports are contradictory to each other since , value of the first report is from recently performed study we would consider that the test material is not classified as per CLP criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.