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EC number: 276-982-1 | CAS number: 72905-97-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014-12-16~2014-12-19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- other company data
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Cuprate(4-), [μ-[[3,3'-[azobis[(2-hydroxy-4,1-phenylene)azo]]bis[4-hydroxy-6-[(3-sulfophenyl)amino]-2-naphthalenesulfonato]](8-)]]di-, ammonium sodium
- EC Number:
- 276-982-1
- EC Name:
- Cuprate(4-), [μ-[[3,3'-[azobis[(2-hydroxy-4,1-phenylene)azo]]bis[4-hydroxy-6-[(3-sulfophenyl)amino]-2-naphthalenesulfonato]](8-)]]di-, ammonium sodium
- Cas Number:
- 72905-97-6
- Molecular formula:
- C44H24Cu2N8O17S4.xNa xNH4, x=0~4 , typical x=2
- IUPAC Name:
- Cuprate(4-), [μ-[[3,3'-[azobis[(2-hydroxy-4,1-phenylene)azo]]bis[4-hydroxy-6-[(3-sulfophenyl)amino]-2-naphthalenesulfonato]](8-)]]di-, ammonium sodium
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report):Everdirect SH14(DBk112)
- Substance type:Powder
- Composition of test material, percentage of components:88.53%
- Lot/batch No.:4401
- Expiration date of the lot/batch:2016-10-31
- Storage condition of test material:Refrigeration(5 ± 3℃)
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 Mix
- Test concentrations with justification for top dose:
- 5, 2.5, 1.25, 0.625, 0.3125 mg/plate
Controls
- Untreated negative controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- 2-acetylaminofluorene
- 4-nitroquinoline-N-oxide
- 9-aminoacridine
- sodium azide
- benzo(a)pyrene
- mitomycin C
- Remarks:
- with or without S9 Mix
Results and discussion
Test results
- Key result
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
Table 1 : Genotype results for 5 strains of Salmonella typhimurium
Test strains |
histidine requirement |
△uvrB mutation |
rfa mutation |
ampicillin resistance |
TA98 |
+ |
+ |
+ |
+ |
TA100 |
+ |
+ |
+ |
+ |
TA102 |
+ |
Θ |
+ |
+ |
TA1535 |
+ |
+ |
+ |
Θ |
TA1537 |
+ |
+ |
+ |
Θ |
Table 2 : Mutagenicity results for Salmonella typhimrium TA98
Test groups |
Test substance (mg/plate) |
Reverse mutagenicity Value (CFU/plate) |
Average colony count |
||
1 |
2 |
3 |
|||
Treated with S9 Mix |
Negative control |
48 |
48 |
42 |
46 ± 3 |
Positive control |
154 |
161 |
174 |
163 ± 10 |
|
Test groups |
|
||||
5 |
33 |
39 |
28 |
33 ± 6 |
|
2.5 |
45 |
31 |
36 |
37 ± 7 |
|
1.25 |
48 |
31 |
45 |
41 ± 9 |
|
0.625 |
41 |
45 |
36 |
41 ± 5 |
|
0.3125 |
41 |
45 |
33 |
40 ± 6 |
|
Not Treated with S9 Mix |
Negative control |
42 |
45 |
41 |
43 ± 2 |
Positive control |
230 |
235 |
220 |
228 ± 8 |
|
Test groups |
|
||||
5 |
34 |
48 |
48 |
43 ± 8 |
|
2.5 |
47 |
41 |
30 |
39 ± 9 |
|
1.25 |
32 |
37 |
41 |
37 ± 5 |
|
0.625 |
47 |
59 |
41 |
49 ± 9 |
|
0.3125 |
36 |
32 |
31 |
33 ± 3 |
Table 3 : Mutagenicity results for Salmonella typhimrium TA100
Test groups |
Test substance (mg/plate) |
Reverse mutagenicity Value (CFU/plate) |
Average colony count |
||
1 |
2 |
3 |
|||
Treated with S9 Mix |
Negative control |
181 |
189 |
235 |
202 ± 29 |
Positive control |
1009 |
901 |
1107 |
1006 ± 103 |
|
Test groups |
|
||||
5 |
261 |
271 |
280 |
271 ± 10 |
|
2.5 |
305 |
247 |
230 |
261 ± 39 |
|
1.25 |
293 |
268 |
282 |
281 ± 13 |
|
0.625 |
263 |
280 |
263 |
269 ± 10 |
|
0.3125 |
275 |
274 |
261 |
270 ± 8 |
|
Not Treated with S9 Mix |
Negative control |
218 |
215 |
234 |
222 ± 10 |
Positive control |
429 |
401 |
423 |
418 ± 15 |
|
Test groups |
|
||||
5 |
207 |
232 |
224 |
221 ± 13 |
|
2.5 |
252 |
213 |
250 |
238 ± 22 |
|
1.25 |
249 |
251 |
222 |
241 ± 16 |
|
0.625 |
265 |
252 |
240 |
252 ± 13 |
|
0.3125 |
270 |
303 |
276 |
283 ± 18 |
Table 4 : Mutagenicity results for Salmonella typhimrium TA102
Test groups |
Test substance (mg/plate) |
Reverse mutagenicity Value (CFU/plate) |
Average colony count |
||
1 |
2 |
3 |
|||
Treated with S9 Mix |
Negative control |
193 |
172 |
176 |
180 ± 11 |
Positive control |
276 |
245 |
265 |
262 ± 16 |
|
Test groups |
|
||||
5 |
164 |
146 |
157 |
156 ± 9 |
|
2.5 |
176 |
163 |
161 |
167 ± 8 |
|
1.25 |
148 |
140 |
121 |
136 ± 14 |
|
0.625 |
153 |
150 |
147 |
150 ± 3 |
|
0.3125 |
129 |
128 |
141 |
133 ± 7 |
|
Not Treated with S9 Mix |
Negative control |
109 |
116 |
109 |
111 ± 4 |
Positive control |
356 |
306 |
294 |
319 ± 33 |
|
Test groups |
|
||||
5 |
102 |
126 |
114 |
114 ± 12 |
|
2.5 |
130 |
124 |
126 |
127 ± 3 |
|
1.25 |
129 |
147 |
129 |
135 ± 10 |
|
0.625 |
131 |
106 |
116 |
118 ± 13 |
|
0.3125 |
136 |
146 |
130 |
137 ± 8 |
Table 5 : Mutagenicity results for Salmonella typhimrium TA1535
Test groups |
Test substance (mg/plate) |
Reverse mutagenicity Value (CFU/plate) |
Average colony count |
||
1 |
2 |
3 |
|||
Treated with S9 Mix |
Negative control |
13 |
12 |
13 |
13 ± 1 |
Positive control |
233 |
220 |
214 |
222 ± 10 |
|
Test groups |
|
||||
5 |
10 |
15 |
13 |
13 ± 3 |
|
2.5 |
13 |
19 |
16 |
16 ± 3 |
|
1.25 |
14 |
10 |
20 |
15 ± 5 |
|
0.625 |
18 |
14 |
19 |
17 ± 3 |
|
0.3125 |
20 |
14 |
20 |
18 ± 3 |
|
Not Treated with S9 Mix |
Negative control |
14 |
18 |
10 |
14 ± 4 |
Positive control |
77 |
80 |
72 |
76 ± 4 |
|
Test groups |
|
||||
5 |
16 |
15 |
12 |
14 ± 2 |
|
2.5 |
13 |
18 |
18 |
16 ± 3 |
|
1.25 |
17 |
14 |
15 |
15 ± 2 |
|
0.625 |
11 |
10 |
8 |
10 ± 2 |
|
0.3125 |
17 |
10 |
10 |
12 ± 4 |
Table 6 : Mutagenicity results for Salmonella typhimrium TA1537
Test groups |
Test substance (mg/plate) |
Reverse mutagenicity Value (CFU/plate) |
Average colony count |
||
1 |
2 |
3 |
|||
Treated with S9 Mix |
Negative control |
8 |
5 |
6 |
6 ± 2 |
Positive control |
376 |
383 |
361 |
373 ± 11 |
|
Test groups |
|
||||
5 |
9 |
5 |
7 |
7 ± 2 |
|
2.5 |
11 |
11 |
8 |
10 ± 2 |
|
1.25 |
8 |
7 |
7 |
7 ± 1 |
|
0.625 |
7 |
8 |
8 |
8 ± 1 |
|
0.3125 |
10 |
8 |
7 |
8 ± 2 |
|
Not Treated with S9 Mix |
Negative control |
7 |
6 |
6 |
6 ± 1 |
Positive control |
354 |
383 |
371 |
369 ± 15 |
|
Test groups |
|
||||
5 |
9 |
9 |
6 |
8 ± 2 |
|
2.5 |
7 |
7 |
10 |
8 ± 2 |
|
1.25 |
8 |
7 |
6 |
7 ± 1 |
|
0.625 |
6 |
5 |
8 |
6 ± 2 |
|
0.3125 |
6 |
4 |
4 |
5 ± 1 |
Applicant's summary and conclusion
- Conclusions:
- No positive criteria was observed when the reverse mutation colonies of all test groups compared with the negative controls, and all test data were within the effective data range. There results indicate that no Salmonella typhimurium reverse mutation occurs.
- Executive summary:
According to the OECD Guideline 471 (Bacterial Reverse Mutation Assay), the result of test is "negative response". Therefore, the hazard category in CLP is not classified.
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