Dinonylnaphthalenedisulphonic acid

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1977

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: study similar to the guideline (OECD 402), but non-GLP and with limited information on the conditions of performance. The information in the report is limited to the information in the summary.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Federal Hazardous Sub­ stances Act (1973)

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Dinonylnaphthalenedisulphonic acid

EC Number:

262-110-7

EC Name:

Dinonylnaphthalenedisulphonic acid

Cas Number:

60223-95-2

Molecular formula:

C28 H44 O6 S2

IUPAC Name:

dinonylnaphthalenedisulphonic acid

Test material form:

not specified

Details on test material:

- Name of test material (as cited in study report): Nacure 155

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: no data (from stock)
- Age at study initiation: mature
- Weight at study initiation:2045-2810 g
- Housing: individually
- Diet: Purina Rabbit Chow ad libitum
- Water: ad libitum
- Acclimation period: not indicated

ENVIRONMENTAL CONDITIONS: no data

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: not indicated
- Type of wrap if used: occlusive, rubber dam

REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes
- Time after start of exposure: 24 hours

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2 mL
- Concentration (if solution): 55%

Duration of exposure:

24 hours

Doses:

2000 mg/kg

No. of animals per sex per dose:

5 males + 5 females

Control animals:

not required

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: daily; weighing at start and on day 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs and body weight

Statistics:

NA

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Effect levels 2

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 1 100 mg/kg bw

Based on:

act. ingr.

Mortality:

none

Clinical signs:

slight erythema and well defined oedema until day 9, thereafter no findings

Body weight:

bodyweight gain within expected ranges

Gross pathology:

no findings

Applicant's summary and conclusion

Interpretation of results:

practically nontoxic

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The LD50 of the test substance is > 1100 mg/kg bw

Executive summary:

Rabbits (5/sex) were dermally exposed to the test substance for 24 hours. No effects other than slight erythema and well defined oedema (not observed after day 9) were reported. Body weight gain was considered normal. Necropsy did not reveal any findings. The LD50 is > 1100 mg/kg bw.