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Reaction mass ofDisodium [1-{[2-(hydroxy-kO)-3,5-dinitrophenyl]diazenyl-kN1}naphthalen-2-olato(2-)-kO][3-(hydroxy-kO)-4-{[2-(hydroxy-kO)naphthalen-1-yl]diazenyl-kN1}-7-nitronaphthalene-1-sulfonato(3-)]chromate(2-) andDisodium [1-{[2-(hydroxy-kO)-3,5-dinitrophenyl]diazenyl-kN2}naphthalen-2-olato(2-)-kO][3-(hydroxy-kO)-4-{[2-(hydroxy-kO)naphthalen-1-yl]diazenyl-kN1}-7-nitronaphthalene-1-sulfonato(3-)]chromate(2-)
EC number: 944-038-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental starting date - 05 October 1993; Experimental termination date - 07 October 1993; Study completion date - 24 January 1994.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Code number for the test material as used in the study report: FAT 20037/C
- Source and batch No.of test material: 9300001
- Expiration date of the batch: February 1998
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
OTHER SPECIFICS:
- Purity: ca. 80 %
- Appearance: solid
- Solubility: >2 g/L (in water) - Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: the testing facility of the Product Ecology TF, Textile Dyes Division, CIBA-GEIGY Ltd., Basle
- Age at study initiation: young daphnids
- Method of breeding: Cultures of Daphnia magna are maintained in glass vessels containing aprox. 3.0 L of reconstituted water at 18 - 22 °C. The water was renewed partially three times weekly. The daphnia were feed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantités that the food was consumed within 24 h.
Pretreatment: 24 hours before the start of the exposure reproductive daphnia were separated from the young daphnia by sieving all individuals through a 710 µrn sieve. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 136 mg/L CaCO3
- Test temperature:
- 21.5 °C
- pH:
- 7.8 - 8.2
- Dissolved oxygen:
- 96-99 %
- Nominal and measured concentrations:
- 58, 100 and 180 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Beaker
- Material, size, headspace, fill volume: 250 mL Glass beaker.
- Volume of solution: 100-200 mL test medium.
- No. of organisms per vessel: 10
- No. of vessels per concentration: Duplicate
- No. of vessels per control: Duplicate
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Bi-distilled water.
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: 4:1
- Culture medium different from test medium: No
OTHER TEST CONDITIONS
- Photoperiod: 16 hours
- pH: 7.8 - 8.2
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Immobilization.
VEHICLE CONTROL PERFORMED: yes: - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- Potassium dichromate (EC50: 0.96 mg/L)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour EC50 of the test substance towards aquatic invertebrates >10 mg/L.
- Executive summary:
The short-term toxicity potential of the test item to aquatic invertebrates was investigated in a study conducted according to OECD Guideline 202 and EU Method C.2, in compliance with GLP. Based on a pretest, the test concentrations were chosen as 58, 100 and 180 mg/L nominal. For each concentration, of test substance, 20 daphnids divided in two groups of 10 animals were used. The daphnids were exposed to the test article in 250 mL beakers containing 100 - 200 mL test medium with 10 animals per beaker. All tests were run in duplicate. During exposure the animals were kept at 21 ± 1 °C with 16 hours illumination. Test evaluations were done according to the guidelines by visual assessment of mobility of the daphnia after 24 and 48 hours. During test duration the test article concentrations in the analysed test media were in the range of 4.8 - 5.6 % of the nominal values. 2.9, 5.5 and 10.0 mg/L were the measured concentrations for nominal concentrations of 58, 100 and 180 mg/L, respectively. Hence, the reported results were related to the analytical concentrations of the test article. In all test concentrations no alteration of the test substance after 48 hours was observed. In the control and in all test concentrations, no immobilization of the daphnids was observed after 48 hours. The EC0 is at least 10 mg/L, but might even be higher than this concentration. The EC50 and EC100 values are clearly higher than 10 mg/L. Therefore, the EC50 value could not be calculated.
Reference
The reported results were related to the analytical concentrations of the test article. During test duration the test article concentrations in the analysed test media were in the range of 4.8 - 5.6 % of the nominal values. 2.9, 5.5 and 10.0 mg/L were the measured concentrations for nominal concentrations of 58, 100 and 180 mg/L, respectively. The test concentrations were prepared from the supernatant of a 1 g/L stock solution, in which most of the substance was settled down. For the preparation of the test concentrations only the supernatant of the stock solution was taken. In all test concentrations non alteration of the test substance after 48 hours was observed. In the control and in all test concentrations, no immobilization of the daphnia was observed after 48 hours. The EC0 is at least 10.0 mg/L, but might even be higher than this concentration. The EC50 and EC100 values are clearly higher than 10.0 mg/L. Therefore, the EC50 value could not be calculated.
Description of key information
The short-term toxicity potential of the test item to aquatic invertebrates was investigated in a study conducted according to OECD Guideline 202 and EU Method C.2, in compliance with GLP.Based on a pre-test, the test concentrations were chosen as 58, 100 and 180 mg/L nominal. For each concentration, of test substance, 20 daphnids divided in two groups of 10 animals were used. The daphnids were exposed to the test article in 250 mL beakers containing 100 - 200 mL test medium with 10 animals per beaker. All tests were run in duplicate. During exposure the animals were kept at 21 ± 1°C with 16 hours illumination. Test evaluations were done according to the guidelines by visual assessment of mobility of the daphnia after 24 and 48 hours. During test duration the test article concentrations in the analysed test media were in the range of 4.8 - 5.6 % of the nominal values. 2.9, 5.5 and 10.0 mg/L were the measured concentrations for nominal concentrations of 58, 100 and 180 mg/L, respectively. Hence, the reported results were related to the analytical concentrations of the test article. In all test concentrations no alteration of the test substance after 48 hours was observed. In the control and in all test concentrations, no immobilization of the daphnids was observed after 48 hours. The EC0 is at least 10 mg/L, but might even be higher than this concentration. The EC50 and EC100 values are clearly higher than 10 mg/L. Therefore, the EC50 value could not be calculated.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 10 mg/L
Additional information
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