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Diss Factsheets
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EC number: 221-341-3 | CAS number: 3071-32-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- January 1979 to 29 December 1980
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: The test material contained components other than 1-phenylethyl hydroperoxide, therefore observed effects cannot be attributed to either 1-phenylethyl hydroperoxide or other components (ethyl benzene or other volatile componetnts).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- not applicable
- Principles of method if other than guideline:
- The study followed the methodologies as in Ames et al., 1975 and is equivalent to the OECD TG 471 (adopted in July 1997).
Ames, B.N., McCann, J. and Yamasaki, E. (1975). Methods for Detecting Carcinogens and Mutagens with the Salmonella/Mammalian-Microsome Mutagenicity Test. Mutation Res., 31, 347-364. - GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1-phenylethyl hydroperoxide
- EC Number:
- 221-341-3
- EC Name:
- 1-phenylethyl hydroperoxide
- Cas Number:
- 3071-32-7
- Molecular formula:
- C8H10O2
- IUPAC Name:
- 1-phenylethane-1-peroxol
- Details on test material:
- - Name of test material (as cited in study report): EBHP plant stream samle (33.9% of ethyl benzene hydroperoxide (EBHP) in ethyl benzene).
- Analytical purity: 33.9%
- Composition of test material, percentage of components: ethylbenzene hydroperoxide plant stream 33.9%, acetophenone 2.95%, phenyl ethyl alcohol 2.40%, benzaldehyde 0.04%, phenol 0.23%, sodium 0.5% and acids (titration) 0.001% Meq/G.
- Purity test date: 28 June 1978.
- Lot/batch No.: 78/12484
- Stability : For at least 4 hours (in DMSO)
Constituent 1
Method
- Target gene:
- Histidine operon (his) for Salmonella.
Tryptophan operon (trp) for E.Coli.
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium, other: S. typhimurium TA 1535, TA 1537, TA 1538, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- E. coli, other: E. coli WP2 and E. coli WP2 uvr A
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver microsomal activation system
- Test concentrations with justification for top dose:
- 0.0678, 0.678, 6.78, 67.8 and 678 µg of EBHP (final amounts of 0.2, 2, 20, 200 and 2000 µg of EBHP plant stream per plate) with or without S9.
- Vehicle / solvent:
- ethyl benzene (33.9% of ethyl benzene hydroperoxide (EBHP) in ethyl benzene)
Controlsopen allclose all
- Untreated negative controls:
- yes
- Remarks:
- (concurrent untreated control)
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- for S. typhimurium with or without S9
Migrated to IUCLID6: 20 µg
- Untreated negative controls:
- yes
- Remarks:
- (concurrent untreated control)
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: neutral red, 20 µg
- Remarks:
- for S. typhimurium with or without S9
- Untreated negative controls:
- yes
- Remarks:
- (concurrent untreated control)
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- for E. coli with or without S9
Migrated to IUCLID6: 20 µg
- Untreated negative controls:
- yes
- Remarks:
- (concurrent untreated control)
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- Remarks:
- for E. coli with or without S9
Migrated to IUCLID6: 20 µg
Results and discussion
Test resultsopen allclose all
- Species / strain:
- other: S. typhimurium TA 1535, TA 1537, TA 1538, TA 98, TA 100 and E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not applicable
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not applicable
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not applicable
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
See attachment tables for results.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive without metabolic activation In E. coli WP2
Application of the EBHP plant stream sample up to 2000 µg per plate did not consistently increase the reverse gene mutation rate of E. coli WP2 uvrA,
S. typhimurium TA 1535, TA 1537, TA 1538, TA 98, and TA I00 in vitro in the presence or absence of a rat liver S9 fmaction, but did result in an increase in the reverse mutation frequency in E. coli WP2 in the absence of the rat liver S9 fraction. Therefore the EBHP is considered to be a a weak
microbial mutagen in E. coli WP2 in the absence of S9 under the conditions of this test. - Executive summary:
The mutagenic activity of an EBHP plant stream sample was investigated in agar layer cultures of Salmonella typhimurium TA 1535, TA 1537, TA 1538, TA 98, TA 100, Escherichia coli WP2 and WP2 uvr both with and without the incorporation of a rat liver microsomal activation system.
Application of the EBHP plant stream sample up to 2000 µg per plate did not consistently increase the reverse gene mutation rate of E. coli WP2 uvrA,
S. typhimurium TA 1535, TA 1537, TA 1538, TA 98, and TA I00 in vitro in the presence or absence of a rat liver S9 fraction, but did result in an increase in the reverse mutation frequency in E. coli WP2 in the absence of the rat liver S9 fraction.
The results with the EBHP plant stream sample indicate that it is a weak microbial mutagen in E. coli WP2 in the absence of S9.
The test material contains only 33.9% of 1-phenylethyl hydroperoxide. There were not enough information to identify observed effects attributed to the substance, therefore a reliability of 3 is assigned.
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