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EC number: 228-819-0 | CAS number: 6359-98-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- From July 31st to August 02nd, 2001
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Justification for type of information:
- Justification for Read Across is reported in the endpoint summary and it is further detailed in the report attached to the IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- part 1
- GLP compliance:
- not specified
- Details on test solutions:
- A solution in dilution water was prepared at the nominal concentration of 125 mg/l. pH of the solution was 8.2.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna, Stratus.
- Age at study initiation: young animals aged 0-2 4 hours were used.
- Food type: Tetra Animin®.
- Frequency of feeding: twice a week.
ACCLIMATION
- Acclimation conditions: daphnids were maintained at 20 ± 1°C and under a photoperiod of 16/8 hours light/dark. 5 organisms per litre. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 d
- Test temperature:
- 20 ± 1 °C
- pH:
- Control: 7.9 - 8.6
Test vessels: 8.2 - 8.3 - Dissolved oxygen:
- Control: 7.8 - 8.8 mg/l
Test vessels: 7.8 - 8.4 - Nominal and measured concentrations:
- 0 and 125 mg/l (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml beaker according to DIN, HF.
- No. of organisms per vessel: 5.
- No. of vessels per concentration: 4 replicates.
- No. of vessels per control: 4 replicates.
- Biomass loading rate: use of 5 animals each in 20 ml of sample.
TEST MEDIUM
- Preparation of dilution water: M4 Medium.
- Composition of M4 Medium: CaCl2 x 2H2O 177.9 mg/l, MgSO4 x 7H2O 67.4 mg/l, NaCl 17.8 mg/l, Na2SiO3 x 9H20 1000 µg/l, NaNO3 27.4 µg/l, KH2PO4 14.3 µg/l, K2HPO4 18.4 µg/l, Na2EDTA x 2H2O 2500 µg/l, FeSO4 x 7H2O 995.5 µg/l, H3BO3 2859.5 µg/l, MnCl2 x 4H2O 380.5 µg/l, LiCl 310.5 µg/l, RbCl 71.0 µg/l, SrCl2 x 6H2O 152.0 µg/l, NaBr 16.0 µg/l, Na2MoO4 x 2H2O 63.0 µg/l, CuCl2 x 6H2O 16.5 µg/l, ZnCl2 13.0 µg/l, CoCl2 x 6H2O 10.0 µg/l, KI 3.25 µg/l, Na2SeO3 2.19 µg/l, NH4VO3 0.575 µg/l, Thiamin x HCl 75.0 ng/l, Cyanocobalamin 1.0 ng/l, Biotin 0.75 ng/l
- Composition of dilution water: CaCl2 x 2H2O 177.9 mg/l, MgSO4 x 7H2O 67.4 mg/l, NaCl 17.8 mg/l.
- Composition of tap water: Ca+ 31.6 mg/l, Mg2+ 5.5 mg/l, K+ 3.2 mg/l, Na+ 11.2 mg/l.
EFFECT PARAMETERS MEASURED
Immobilization after 48 hours. - Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 125 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Remarks:
- ion the control not more than 10 % of the Daphnia have been immobilized and the dissolved oxygen concentration at the end of the test was higher than 60 %
- Conclusions:
- EC50 (48h) > 125 mg/l (nominal)
- Executive summary:
Method
The short term toxicity to aquatic invertebrates was determined using the OECD Guideline 202, part I (Daphnia sp. Acute Immobilisation Test). A solution in dilution water was prepared at the nominal concentration of 125 mg/l. pH of the solution was 8.2.
Results
EC50 (48h) > 125 mg/l (nominal)
Reference
Test results
Replicate | Conc. (mg/l) | N. of daphnids | Inmmobilization after 24 hrs | Inmmobilization after 48 hrs | ||
Absolute | Percentage | Absolute | Percentage | |||
Blank 1 | 0 | 5 | 0 | 5 | 0 | 5 |
Blank 2 | 0 | 5 | 1 | 1 | ||
Blank 3 | 0 | 5 | 0 | 0 | ||
Blank 4 | 0 | 5 | 0 | 0 | ||
Test item 1 | 125 | 5 | 0 | 0 | 0 | 0 |
Test item 2 | 125 | 5 | 0 | 0 | ||
Test item 3 | 125 | 5 | 0 | 0 | ||
Test item 4 | 125 | 5 | 0 | 0 |
Summary of the test conditions
Conc. (mg/l) | pH | O2 (mg/l) | ||
0 hrs | 48 hrs | 0 hrs | 48 hrs | |
0 | 7.9 | 8.6 | 8.8 | 7.8 |
125 | 8.2 | 8.3 | 8.4 | 7.8 |
Description of key information
EC50 (48h) > 125 mg/l (nominal)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 125 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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