2-Propenamide, 3-(1,3-benzodioxol-5-yl)-N,N-diphenyl-, (2E)-

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11 May - 15 August 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

according to OECD 203 (1992), China guideline 203 (2013) and GLP

Qualifier:

according to guideline

Guideline:

other: China guideline 203

Version / remarks:

2013

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 10 mg/L
- Sampling at 24-hour intervals

Vehicle:

no

Details on test solutions:

Test item was weighed and dissolved with test water to make up test solution of 10 mg/L. The solution was mixed with magnetic stirrer using a stirring rate about 420 rpm about 24 hour at room temperature and filtrated with 0.45 µm micro membrane filter before exposure.

Test organisms (species):

other: Gobiocypris rarus

Details on test organisms:

TEST ORGANISM
- Common name: Rare minnow (Gobiocypris rarus)
- Strain: Rare minnow20150826
- Source: Shanghai Academy of Public Measurement
- Age: about 1 year old
- Length at study initiation: 3.6 - 3.9 cm
- Weight at study initiation: 0.4314 - 0.7142 g

ACCLIMATION
- Acclimation conditions: same as test
- Type food during acclimation: brine shrimp
- Feeding frequency during acclimation: twice on workday and one on weekend
- Health during acclimation: healthy, no mortality observed

FEEDING DURING TEST: none

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

none

Hardness:

140 mg/L (as CaCO3)

Test temperature:

23.12 - 24.61 °C

pH:

7.90 - 8.08

Dissolved oxygen:

64.2 - 95.9 %

Nominal and measured concentrations:

Nominal: 10 mg/L
Geometric mean measured: 0.0943 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 L rectangle glass tanks (length 30 cm, width 20 cm height 30 cm)
- Type: not specified
- Aeration: before usage
- Renewal rate of test solution: 48 h
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: < 1.0 g fish/L water

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ground water treated with Aquapro pure water system
- Culture medium different from test medium: no
- Intervals of water quality measurement: measured at the beginning, at the end and every 24 h throughout the exposure

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12 hours light : 12 hours dark
- Light intensity: not specified

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Range finding study: yes
- Test concentration (definitive test): 10 mg/L (nominal)
- Results used to determine the conditions for the definitive study: results of range finding test

Reference substance (positive control):

yes

Remarks:

Potassium dichromate, LC50 (24 h) = 464.31 mg/L (95 % confidence limits: 407.28 - 529.32 mg/L)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.094 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Other biological observations: none, fish showed no poisoning signs or deaths in control vessels and test vessels
- Mortality of control: 0
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: not reported

Results with reference substance (positive control):

Potassium dichromate, LC50 (24 h) = 464.31 mg/L (95 % confidence limits: 407.28 - 529.32 mg/L), test performed in a separate study

Reported statistics and error estimates:

The effects of the limit concentration was determined directly from the test results.

Sublethal observations / clinical signs:

Table 1: Cumulative mortality

Nominal concentration [mg/L]	Cumulative mortality [%]
	3 h	6 h	24 h	48 h	72 h	96 h
0	0	0	0	0	0	0
10	0	0	0	0	0	0

The results are based on geometric mean measured results, i.e., 0.0943 mg/L.

Validity criteria fulfilled:

yes

Remarks:

No fish died in the control group. The dissolved oxygen concentration was 64.2 to 95.9 % of ASV during the test period.

Conclusions:

The 96-hour LC50 on rare minnow (Gobiocypris rarus) was greater than the limit of solubility (> 0.0943 mg/L).

Executive summary:

An experimental study was conducted according to OECD 203 and GLP under semi-static conditions as limit test. The limit test was performed with saturated test solution  (nominal 10 mg/L). The test concentration was analysed by UPLC/UV from fresh and aged test solutions. The effect concentrations were based on the geometric mean measured values (0.0943 mg/L). After 96 h no mortality was observed. Thus, the LC₅₀ (96 h) was estimated to be > 0.0943 mg/L (mean measured), the limit of solubility.

Description of key information

LC₅₀ (96 h): > 0.0943 mg/L based on mortality of Gobiocypros rarus.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 0.094 mg/L

Additional information

One experimental study was conducted according to OECD 203 and GLP under semi-static conditions as limit test. The limit test was performed with saturated test solution  (nominal 10 mg/L). The test concentration was analysed by UPLC/UV from fresh and aged test solutions. The effect concentrations were based on the geometric mean measured values (0.0943 mg/L). After 96 h no mortality was observed. Thus, the LC₅₀ (96 h) was estimated to be > 0.0943 mg/L (mean measured), the limit of solubility.