Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 211-802-7 | CAS number: 696-99-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- October - November 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentration: 100 mg/l
- Vehicle:
- no
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Strain: HAMILTON BUCHANAN
- Source: Charles River (Sulzfeld)
- Age at study initiation: about 6 month
- Length at study initiation: 2.5 - 3.5 cm
ACCLIMATION
- Medical treatment: none
- Keeping water: synthetic fresh water in accordance with ISO
- Mortality while kept: < 2% - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No mortalities or sub-lethal effects of exposure were observed.
- Hardness:
- 14.2 °dH
- Test temperature:
- 22.1 - 22.4 °C
- pH:
- 7.4 - 7.6
- Dissolved oxygen:
- 8.0 - 9.3 mg / l
- Salinity:
- no data reported.
- Nominal and measured concentrations:
- 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 x 135 x 200 mm
- Fill volume: 5 l test medium
- Aeration: ventilated
- Type: static
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water in accordance with ISO
OTHER TEST CONDITIONS
- Photoperiod: 16 h light / 8 h dark
TEST CONCENTRATIONS
- Test concentration: 100 mg / l - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: behaviour, body length and weight
- Details on results:
- No mortalities or sub-lethal effects of exposure were observed for the test concentration of 100 mg / l.
- Reported statistics and error estimates:
- The above result refers to nominal concentration as a direct relationship between measured TOC values and C-containing components of the test substance (= benzene amine) which cannot be assessed quantitatively. On the other hand, in aqueous solutions benzene amine reacts to benzene aldehyde. As both components have nearly identical molecular weights, the TOC values, fairly constant throughout the test period, reflect rather constant exposure rates of the sum of both chemical bodies, benzene amine and benzene aldehyde, respectively.
- Sublethal observations / clinical signs:
Visible effects on appearance and behaviour of fish
Control substance:
after 2 hours 10 animals normal swimming action after 24 hours 10 animals normal swimming action after 48 hours 10 animals normal swimming action after 72 hours 10 animals normal swimming action after 96 hours 10 animals normal swimming action Test substance, 100 mg/l:
after 2 hours 10 animals normal swimming action after 24 hours 10 animals normal swimming action after 48 hours 10 animals normal swimming action after 72 hours 10 animals normal swimming action after 96 hours 10 animals normal swimming action Body length and weight after sacrificing at end of study
Test fish Body length [cm] at control and test substance Control 100 mg test substance / l 1 2.8 2.6 2 3.1 3.2 3 3.0 3.5 4 3.1 3.3 5 2.5 2.6 6 3.0 3.4 7 2.5 2.9 8 2.9 2.9 9 2.8 3.5 10 3.3 3.5 fresh weight [g] of 10 fishes 2.18 2.7 - Validity criteria fulfilled:
- yes
- Conclusions:
- The study is regarded as a valid guideline study with GLP compliance. According to the reported LC 100 value (> 100 mg/L) the test substance is not classified as toxic to fish.
- Executive summary:
The acute toxicity of the substance (benzylamine)trifluoroborone was investigated according to EU Method C.1 in compliance with GLP. Brachydanio rerio (Zebra fish) was used as test organisms and where exposed for 96 hours to the test substance. A control was included, no reference substance is mentioned. It was a static freshwater test with a temperature range of 22.1 °C - 22.4 °C (control: 21.9 °C - 22.4 °C) and a pH range of 7.4 - 7.6 (control: 7.5 - 7.7) and a substance concentrations of 100 mg/L. Direct weighing was the method of administration with a 16 hours light / 8 h dark cycle during the test period. As result a LC 100 of > 100 mg/L is reported.
Reference
Description of key information
EU Method C.1, GLP, Brachydanio rerio (Zebra fish), static freshwater, LC 100 > 100 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
The acute toxicity of the substance (benzylamine)trifluoroborone was investigated according to EU Method C.1 in compliance with GLP.Brachydanio rerio(Zebra fish) was used as test organisms and where exposed for 96 hours to the test substance. A control was included, no reference substance is mentioned. It was a static freshwater test with a temperature range of 22.1 °C - 22.4 °C (control: 21.9 °C - 22.4 °C) and a pH range of 7.4 - 7.6 (control: 7.5 - 7.7) and a substance concentrations of 100 mg/L. Direct weighing was the method of administration with a 16 hours light / 8 h dark cycle during the test period. As result a LC 100 of > 100 mg/L is reported.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)