2,5,6-trimethylcyclohex-2-en-1-one

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

1969

Report date:

1969

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Principles of method if other than guideline:

Study was performed acc. to internal BASF method, which was in large part equivalent to OECD 405
Deviations from OECD 405: 2 test animals; eye examination 24 hrs before testing not mentioned

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,5,6-trimethylcyclohex-2-en-1-one

EC Number:

243-473-0

EC Name:

2,5,6-trimethylcyclohex-2-en-1-one

Cas Number:

20030-30-2

Molecular formula:

C9H14O

IUPAC Name:

2,5,6-trimethylcyclohex-2-en-1-one

Details on test material:

- Name of test material (as cited in study report): 2,5,6-Trimethyl-2-cyclohexen-1-on
- Analytical purity: 98 - 99%

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated left eye of same animal

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.05 ml
- Concentration (if solution): 100%

Observation period (in vivo):

8 days

Number of animals or in vitro replicates:

2

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no washing

SCORING SYSTEM:
Conjunctival redness, chemosis and corneal opacity were scored according to the below described system.
Although the results in the report were originally not given as Draize scores the data can be transferred into the Draize scoring system (in brackets). (+) = none - negligible effect (Draize score 0)
+ = slight effect (1)
++ = moderate effect (2)
+++ = severe effect (> = 3)

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: 24h

Score:

ca. 1

Max. score:

2

Reversibility:

fully reversible within: 8 days

Remarks on result:

other: only results at 24h, not 24-72h

Any other information on results incl. tables

Results:

Readings	Animal	cornea	Iris	conjunctiva			Symptons
		opacity		redness	swelling	discharge
1h	1	1	0	2	3	0
	2	1	0	2	2	0
24 h	1	1	0	2	1	0
	2	1	2	2	1	0
8 d	1	0	0	0	0	0
	2	0	0	0	0	0

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Due to resulted maximum erythema scoring = 2 at 24 h and the full reversiblity of all effects, no classification is necessary.