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EC number: 205-047-2 | CAS number: 132-16-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed using standard test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Principles of method if other than guideline:
- Short term toxicity of test chemical to aquatic algae was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- DMSO
- Details on test solutions:
- The stock solution 20 g/l was prepared by dissolving dark blue powder in DMSO. Test solutions 21 mg/l were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. It was not possible to test at higher concentration because of formation of precipitation.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green algae
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml
- Method of cultivation: No data available
ACCLIMATION - No data available
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed: - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- ±1 hour
- Test temperature:
- 23±2°C
- pH:
- Test at highest concentration: 7.9 (Change to 7.7 during tests)
Control: 8.2 (changed to 8 during test)
Control + DMSO: 8 (changed to 7.7 during test) - Nominal and measured concentrations:
- Limit test performed at 21 mg/l.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 15 ml
- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: IC
- Remarks:
- %
- Effect conc.:
- 21 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 1.6 % inhibition were observed at 21 mg/l.
- Results with reference substance (positive control):
- Results with reference substance valid
- EC50: 0.75 mg/L - Reported statistics and error estimates:
- The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the growth rate inhibition of Desmodesmus subspicatus due to the exposure of test chemical for 72 hrs, 1.6 % inhibition was observed at 21 mg/l limit concentration.
- Executive summary:
Aim of this study was to determine the freshwater algal growth inhibition test on the growth of Desmodesmus subspicatus with the test substance. Test was conducted according to OECD Guideline 201. The stock solution 20 g/l was prepared by dissolving dark blue powder in DMSO. Test solutions 21 mg/l were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. It was not possible to test at higher concentration because of formation of precipitation. Test conducted at limit test concentration 21 mg/l. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect was calculated. The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05. Based on the growth rate inhibition of Desmodesmus subspicatus due to the exposure of chemical for 72 hrs, 1.6 % inhibition was observed at 21 mg/l limit concentration after the exposure of 72hrs.
Reference
Description of key information
Aim of this study was to determine the freshwater algal growth inhibition test on the growth of Desmodesmus subspicatus with the test substance. Test was conducted according to OECD Guideline 201. The stock solution 20 g/l was prepared by dissolving dark blue powder in DMSO. Test solutions 21 mg/l were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. It was not possible to test at higher concentration because of formation of precipitation. Test conducted at limit test concentration 21 mg/l. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect was calculated. The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05. Based on the growth rate inhibition of Desmodesmus subspicatus due to the exposure of chemical for 72 hrs, 1.6 % inhibition was observed at 21 mg/l limit concentration after the exposure of 72hrs.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 21 mg/L
Additional information
Aim of this study was to determine the freshwater algal growth inhibition test on the growth of Desmodesmus subspicatus with the test substance. Test was conducted according to OECD Guideline 201. The stock solution 20 g/l was prepared by dissolving dark blue powder in DMSO. Test solutions 21 mg/l were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. It was not possible to test at higher concentration because of formation of precipitation. Test conducted at limit test concentration 21 mg/l. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect was calculated. The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05. Based on the growth rate inhibition of Desmodesmus subspicatus due to the exposure of chemical for 72 hrs, 1.6 % inhibition was observed at 21 mg/l limit concentration after the exposure of 72hrs.
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