sodium 2,4-dihydroxy-3,3-dimethylbutanoate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study; analogy to structural similar substance (sodiumpanthoate)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

The study was performed according to German national standard DIN 38 412, part L1 and Part L15, 1982, which is comparable to OECD guideline 203.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

sodium (2R)-2,4-dihydroxy-3,3-dimethyl-butanoate

Cas Number:

60979-68-2

Molecular formula:

C6 H11 Na O4

IUPAC Name:

sodium (2R)-2,4-dihydroxy-3,3-dimethyl-butanoate

Details on test material:

Butanoic acid, 2,4-dihydroxy-3,3-dimethyl, D-, sodium salt (Natriumpantoat)
Batch No.: 597/13/88
purity: > 98 %

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

Test fish:
Leuciscus idus L. (golden variety, Goldenorfe)
Supplier: Fish farm Paul Eggers, 2354 Hohenwestedt, Germany
Body length: 8.1 cm (range 7.6 - 8.5 cm)
Body weight: 5.0 g (range 3.9 - 5.9 g)
Corpulence factor of the test fish batch: 1.0

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

20 - 21 °C

pH:

7.2 - 10.4

Dissolved oxygen:

7.2 - 8.8 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

2 200 - 4 600 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

4 600 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

48 h-LC50 of the positive control chloroacetamide: about 31 mg/L (corresponds to normal sensitivity).

During the 96 h exposure to Natriumpantoat, mortalities were as follows:
------------------------------------------------------------------------

Nominal test No. of No. of dead fish after
concentration fish 1h 4h 24h 48h 72h 96h
(mg/l)
Control 10 0 0 0 0 0 0
1000 10 0 0 0 0 0 0
2150 10 0 0 1 1 1 1
4640 10 0 8 10 10 10 10
10000 10 10 10 10 10 10 10

10000* 10 2 10 10 10 10 10


Symptoms of intoxication:
-------------------------

Nominal test Symptoms after
concentration 1h 4h 24h 48h 72h 96h
(mg/l)
Control - - - - - -
1000 - - - - - -
2150 - - - - - -
4640 NT N + + + +
10000 + + + + + +

10000* N + + + + +

Definitions of Symptoms:
-: No symptoms
N: Narcotic-like state
T: Tumbling
+: All fish dead

* = test solution after pH-adjustment


Evaluation:
------------
The LD50 was statistically evaluated as the geometrical mean of the LD0 value (1000 mg/l) and the LD100 value (4640 mg/l). All results refer to nominal test concentrations of the test substance.