Hexachloroacetone

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Acute Toxicity of Some Perhalogenated Acetones

Author:

Joseph F. Borzelleca, David Lester

Year:

1965

Bibliographic source:

TOXICOLOGY AND APPLIED PHARMACOLOGY 7, 592-597 (1965)

Materials and methods

Principles of method if other than guideline:

Adult, male, albino rabbits,averaging 2 kg in weight, were used, usually 10 al each of 3 or 4 dosagee of a compound. The hair from the trunk of the
animal was removed by means of an electric dipper. A rubber girdle was fitted, and the rabbit was placed in an individual restraining stock
(Draize et al., 1944) for the 24-hour exposure period. The test liquid was then injected under the girdle and permitted to remain in contact with the
skin for 24 hours. At the end of the contact period, the girdles were removed, the skin was wiped dry, and the rabbits were examined and returned to individual cages. They were maintained under observation at 23 ± 1 °c for 4-6 weeks, during which they were fed a commercial chow.
The LD50 values were calculated by the minimum approximate chi-square normit method of Berkson (1955).

GLP compliance:

not specified

Test type:

other: min. 3 dose groups

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Hexachloroacetone

EC Number:

204-129-5

EC Name:

Hexachloroacetone

Cas Number:

116-16-5

Molecular formula:

C3Cl6O

IUPAC Name:

hexachloropropan-2-one

Details on test material:

Boiling point: 204 °C
Specific gravity: 1.73 (25°C)

Test animals

Species:

rabbit

Strain:

not specified

Sex:

male

Details on test animals or test system and environmental conditions:

Adult, male, albino rabbits,averaging 2 kg in weight, were used, usually 10 al each of 3 or 4 dosagee of a compound.
The hair from the trunk of the animal was removed by means of an electric clipper.

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

The hair from the trunk of the
animal was removed by means of an electric dipper. A rubber girdle was fitted, and the rabbit was placed in an individual restraining stock
(Draize et al., 1944) for the 24-hour exposure period. The test liquid was then injected under the girdle and permitted to remain in contact with
the skin for 24 hours. At the end of the contact period, the girdles were removed, the skin was wiped dry, and the rabbits were examined and
returned to individual cages. They were maintained under observation at 23 ± 1 °c for 4-6 weeks, during which they were fed a commercial chow.

Duration of exposure:

24h

Doses:

no data

No. of animals per sex per dose:

10 at each of 3 or 4 dosages of a compound

Control animals:

not required

Details on study design:

A rubber girdle was fitted, and the rabbit was placed in an individual restraining stock (Draize et al., 1944) for the 24-hour exposure period. The test liquid was then injected under the girdle and permitted to remain in contact with the skin for 24 hours. At the end of the contact period, the girdles
were removed, the skin was wiped dry, and the rabbits were examined and returned to individual cages. They were maintained under observation at 23 ± 1°C for 4 - 6 weeks, during which they were fed a commercial chow.

Statistics:

The LD50 values were calculated by the minimum approximate chi-square normit method of Berkson (1955).

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

1 900 - 4 060 mg/kg bw

Based on:

test mat.

Mortality:

Mild central nervous system depression preceded death, but no other abnormal signs were evident.

Clinical signs:

other: There was no measurable amount of material remaining under the girdle at the end of the exposure period, The exposed area appeared erythematous and was very clearly delineated from the adjacent unexposed areas. These exposed areas then became scaly or de

Gross pathology:

No abnormalities were present on gross autopsy except for tbe discoloration and edema of the exposed skin.

Applicant's summary and conclusion

Interpretation of results:

Category 5 based on GHS criteria

Remarks:

Migrated information

Conclusions:

LD50 = 2980 +/- 1080 mg/kg bw (determined with 30-40 albino rabbits).

Executive summary:

The acute LD50 of dermal administration is presented. The compound was administrated by dermal application (occlusive, 24 h) to rabbits. The compound caused some depression of the central nervous system, but otherwise, no specific toxic signs were associated with lethal doses. Dermal application did not lead to pulmonary damage from hexachloroaceton.

The mechanism of the lethal action of the latter compounds is not apparent in these studies.

In the latter case, the pulmonary absorption is less than half the gastrointestinal absorption. These calculations are interpreted as evidence that the lethal action of these compounds excluding hexachloroacetone is manifested entirely systemically.