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EC number: 211-219-8 | CAS number: 634-93-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
The skin sensitization potential of target chemical 2,4,6-trichloroaniline (CAS No: 634-93-5) was assessedin various experimental studies which were conducted on humans and guinea pigs for target chemical2,4,6-trichloroaniline (CAS No: 634-93-5) andits structurally similar read across substances.Based on the available data for the target and read across substances and applying the weight of evidence approach, it can be concluded that chemical 2,4,6-trichloroaniline (CAS No: 634-93-5) is able to cause skin sensitization and thus can be considered as sensitizing. Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Skin sensitizer”.
Key value for chemical safety assessment
Skin sensitisation
Link to relevant study records
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from safety assessment report
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Skin sensitization study was conducted on guinea pigs for test chemical 2,4,6-trichloroaniline (CAS No: 634-93-5) to determine its sensitization potential.
- GLP compliance:
- not specified
- Type of study:
- not specified
- Justification for non-LLNA method:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 2,4,6-trichloroaniline
- Molecular formula: C6-H4-Cl3-N
- Molecular weight: 196.464 g/mol
- Smiles notation: c1(c(cc(Cl)cc1Cl)Cl)N
- InChl (if other than submission substance): 1S/C6H4Cl3N/c7-3-1-4(8)6(10)5(9)2-3/h1-2H,10H2
- Substance type: organic
- Physical state: Solid - Species:
- guinea pig
- Strain:
- not specified
- Sex:
- not specified
- Details on test animals and environmental conditions:
- not specified
- Route:
- other: not specified
- Route:
- other: not specified
- No. of animals per dose:
- not specified
- Details on study design:
- not specified
- Challenge controls:
- not specified
- Reading:
- 1st reading
- Group:
- test chemical
- Dose level:
- not specified
- Clinical observations:
- Moderate skin sensitization was observed in treated guinea pigs.
- Remarks on result:
- positive indication of skin sensitisation
- Cellular proliferation data / Observations:
- Moderate skin sensitization was observed in treated guinea pigs.
- Interpretation of results:
- other: sensitizing
- Conclusions:
- The test material 2,4,6-trichloroaniline (CAS No: 634-93-5) was considered to be sensitizing to the skin of guinea pigs.
- Executive summary:
Skin sensitization study was conducted on guinea pigs for test chemical 2,4,6-trichloroaniline (CAS No: 634-93-5) to determine its sensitization potential.
When the guinea pigs were treated with test chemical, moderate skin sensitization was observed in treated guinea pigs.
Therefore the chemical 2,4,6-trichloroaniline (CAS No: 634-93-5) was considered to be sensitizing to the skin of guinea pigs.
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (sensitising)
- Additional information:
Skin sensitization:
Various studieshas been investigated for the test chemical2,4,6-trichloroaniline (CAS No: 634-93-5)to observe the potential for skin sensitization to a greater or lesser extent. The studies are based on in vivo experiments in guinea pigs and humans for target chemical2,4,6-trichloroaniline (CAS No: 634-93-5) and its structurally similar read across substanceswhich have beensummarized as below;
Skin sensitization study was reported by safety assessment report on guinea pigs for test chemical 2,4,6-trichloroaniline (CAS No: 634-93-5) to determine its sensitization potential. When the guinea pigs were treated with test chemical, moderate skin sensitization was observed in treated guinea pigs. Therefore the chemical 2,4,6-trichloroaniline (CAS No: 634-93-5) was considered to be sensitizing to the skin of guinea pigs.
Another Skin sensitization study was conducted for test chemical 2,4,6-trichloroaniline (CAS No: 634-93-5) on humans. The chemical have been suspected to cause skin sensitization in humans. Therefore the chemical 2,4,6-trichloroaniline (CAS No: 634-93-5) was considered to be sensitizing to the skin of humans.
The above results were supported by the Guinea pig maximization test conducted by authoratitave database on guinea pigs for similar read across chemical according to OECD Guide–line 406 "Skin Sensitization". The sample was formulated in propylene glycol. The induction concentrations were 2.5 % for the intradermal induction and 50 % for the topical induction. The first provocation was carried out with a 50 % 3,4-dichloroaniline solution. Effects on the skin were seen in all 20 animals of the test substance group and in 9 of 10 animals from the control group. As a result of the reaction in nearly all animals of the control group, the concentration of 50 % is to be regarded as irritative, and therefore a second provocation with concentrations of 5 % and 25% was carried out. The provocation concentration of 25% again produced irritative effects (effects on the skin in 19 of 20 animals of the test substance group, and in 8 of 10 animals from the control group), while the effects were only slight after 5 % (effects on the skin in 1 of 10 animals from the control group). The provocation concentration of 5 % caused effects on the skin in 14 of 20 animals of the test substance group. Therefore, on the basis of observed effects, the test chemical was considered to be sensitizing to the skin of guinea pigs.
The overall results were further supported by the Local Lymph Node Assay (LLNA) conducted by peer reviewed journal on Female CBA/Ca mice to assess the skin sensitization potential of test chemical. The LLNA was conducted on groups of CBA/Ca mice (8-12 weeks of age) by mean of topical application of chemical on the dorsum of both ears at a dose of 25µl of one of three concentrations (0.25%, 0.5% and 1.0%)of the test chemical. 5 days after the first topical application, all mice were injected iv with 250µl phosphate buffered saline (PBS) containing 20µCi of [3H]methyl thymidine (3HTdR) .The mice were killed 5 hr later and the draining auricular lymph nodes excised and pooled for each experimental group. A single cell suspension of lymph node cells (LNC) was prepared by gentle disaggregation through 200 mesh stainless steel gauze. Pooled LNC were pelleted by centrifugation at 190g for 10 min, washed twice with 10 ml PBS and resuspended in 3 ml 5% trichloroacetic acid (TCA). After incubation overnight at 4°C, the precipitate was recovered by centrifugation, resuspended in 1ml 5% TCA and transferred to 10 ml scintillation fluid. Incorporation of3HTdR was measured by p-scintillation counting.The proliferative response of LNC was expressed as mean radioactive disintegrations per minute per lymph node (dpm/node for each experimental group and as the ratio of3HTdR incorporation into LNC of test nodes relative to control nodes [test: control (T:C) ratio]. A chemical was regarded as a sensitizer in the LLNA if at least one concentration resulted in a T:C ratio of 3 or greater and the data were not incompatible with a biological dose response. The test: control (T:C) ratios were 11.2, 14.4 and 18.0 at concentration of 0.25%, 0.5% and 1.0% respectively. Since the resulted test: control (T:C) ratio was greater than 3 at each concentration, the test chemical was considered to be sensitizing in Local Lymph Node Assay (LLNA).
Based on the available data for the target and read across substances and applying the weight of evidence approach,it can be concluded that thechemical 2,4,6-trichloroaniline (CAS No: 634-93-5) is able to cause skin sensitization and considered as sensitizing to the skin.Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Skin Sensitizer”.
Respiratory sensitisation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Justification for classification or non-classification
The skin sensitization potential of test substance 2,4,6-trichloroaniline (CAS No: 634-93-5) andits structurally similar read across substanceswere observed in various studies. From the results obtained from these studies it is concluded that the chemical 2,4,6-trichloroaniline (CAS No: 634-93-5) is likely to cause skin sensitization and hence can be classified as Skin Sensitizer.
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