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EC number: 478-270-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006-10-12 to 2007-06-19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study performed according to OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test) and EC Directive 88/302, ‘Biodegradation - Activated Sludge Respiration Inhibition Test’ without deviations.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Remarks:
- : The testing facility indicated that there were no deviations from the protocol.
- Qualifier:
- according to guideline
- Guideline:
- other: EC Directive 88/302, ‘Biodegradation - Activated Sludge Respiration Inhibition Test’
- Deviations:
- no
- Remarks:
- : The testing facility indicated that there were no deviations from the protocol.
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- : Certificate issued by the United Kingdom Department of Health.
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- - Name of test material (as cited in study report): VRT-753136
- Molecular formula (if other than submission substance): Not applicable
- Molecular weight (if other than submission substance): Not applicable
- Smiles notation (if other than submission substance): Not applicable
- InChl (if other than submission substance): Not applicable
- Structural formula attached as image file (if other than submission substance): Not applicable
- Substance type: No data
- Physical state: White powder
- Analytical purity: 99.4% w/w by High Performance Liquid Chromatography (HPLC)
- Impurities (identity and concentrations): individual unknown components were detected at 0.03 area%; isopropanol (residual solvent) at 300 ppm
- Composition of test material, percentage of components: Not applicable
- Isomers composition: Not applicable
- Purity test date: 2006-05-25
- Lot/batch No.: 25515
- Expiration date of the lot/batch: 2008-05-01
- Stability under test conditions: No data
- Storage condition of test material: Room temperature
- Other: No data
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Sampling and analysis
- Analytical monitoring:
- not specified
- Details on sampling:
- Not applicable
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
- Method: The result of preliminary solubility trials showed that the test substance was sufficiently soluble in dechlorinated water to allow the preparation of suitable aqueous stock solutions. Therefore, test concentrations were established by the addition of appropriate volumes of stock solutions at 2.5 g/L in the preliminary test and 16 g/L in the definitive test. The pH of each stock solution was measured and no adjustment was necessary. Additions of synthetic sewage and microbial inoculum, made at fifteen-minute intervals, gave a final volume of 500 mL.
- Eluate: not applicable
- Differential loading: not applicable
- Controls: yes (16 mL synthetic sewage + 284 mL water + 200 mL inoculum)
- Chemical name of vehicle: dechlorinated tap water (hardness, 200-250 mg/L as CaCO3)
- Concentration of vehicle in test medium: 34-268.4 mL per 500 mL final volume
- Evidence of undissolved material: no data
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: Not applicable
- Method of cultivation: No data
- Preparation of inoculum for exposure: On the day of collection, aliquots (25 mL) of the activated sludge were filtered through dried and preweighed Whatman GF/C filter papers which were then dried again at approximately 105 deg C for at least one hour, allowed to cool in a desiccator and reweighed. The mixed liquor suspended solids (MLSS) content of the activated sludge was then calculated and adjusted to 4 g/L by the removal of supernatant (in the preliminary test only). Synthetic sewage (50 mL/L) was added to each stock of activated sludge and these were aerated overnight. On the day of the test, the MLSS content of the sludge was determined and adjusted to 4 g/L by the addition of dechlorinated tap water. The pH and temperature of the sludge were also measured.
- Pretreatment: No
- Initial biomass concentration: 4 g/L
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- None
Test conditions
- Hardness:
- 200-250 mg/L CaCO3
- Test temperature:
- - Control test: 20 deg C
- Preliminary test: 20+-2 deg C
- Definitive test: 19.9+-2 deg C - pH:
- - Control test: 7.6-8.1
- Preliminary test: 7.5-8.3
- Definitive test: 7.4-8.1 - Dissolved oxygen:
- - Control test: 6.1 mg O2/L (t=0); 2.5 mg O2/L (t=3h)
- Preliminary test: 6.5 mg O2/L (t=0); 2.5 mg O2/L (t=3h)
- Definitive test: 6.5-7.2 mg O2/L (T0) 2.5-4 mg O2/L (t=3h) - Salinity:
- No data
- Nominal and measured concentrations:
- - Preleminary test: 10, 100, 1000, 1000, 1000 mg/L (Nominal concentration)
- Definitive test: 500, 1000, 2000, 4000 and 8000 mg/L (Nominal concentrations)
- Positive control: 3, 10 and 32 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: Thermostatically controlled water bath
- Material, size, headspace, fill volume: No data
- Aeration: Yes, using a glass aerator connected to a laboratory supply of oil-free compressed air (ca. 1 L/min)
- Type of flow-through: Not applicable
- Renewal rate of test solution: Not applicable
- No. of organisms per vessel: No data
- No. of vessels per concentration (replicates): No data
- No. of vessels per control (replicates): No data
- No. of vessels per vehicle control (replicates): No data
- Biomass loading rate: No data
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water that had been softened and treated by reverse osmosis and then purified to give a nominal resistivity >= 18 MegOhm.cm
- Total organic carbon: No data
- Particulate matter: No data
- Metals: No data
- Pesticides: No data
- Chlorine: No data
- Alkalinity: No data
- Ca/mg ratio: No data
- Conductivity: No data
- Culture medium different from test medium: No data
- Intervals of water quality measurement: No data
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: No data
- Light intensity: No data
EFFECT PARAMETERS MEASURED:
- The rate of oxygen consumption was measured, over a period of approximately 10 minutes or until the dissolved oxygen concentration fell below 2 mgO2/L.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: No data
- Justification for using less concentrations than requested by guideline: Not applicable
- Range finding study: Yes
- Test concentrations: Nominal test concentrations were 0, 100 and 1000 mg/L (highest concentration tested in triplicate).
- Results used to determine the conditions for the definitive study: The results of preliminary solubility trials showed that the test substance was sufficiently soluble in dechlorinated water to allow the preparation of suitable aqueous stock solutions. - Reference substance (positive control):
- yes
- Remarks:
- : 3,5-dichlorophenol (3,5-DCP), 99.2%
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9 690 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CI: 6820-16,000 mg/L
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 356 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CI: 171-614 mg/L
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No data
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- - Results with reference substance valid: Yes. Sludge respiration rates were progressively reduced in the presence of increasing concentrations of 3,5-DCP.
- Relevant effect levels: The 3-h EC50 for 3,5-DCP was calculated to be 11.6 mg/L (95% confidence limits, 9.1 - 14.9 mg/L) in the preliminary test, and 9.3 mg/L (95% confidence limits, 7.8 - 11.0 mg/L) in the definitive test. The specific respiration rates of control cultures established at the end of the two test series (26.3 and 24.6 mgO2/g/h) were 107% and 98% of the rates of those established at the start (24.6 and 25.0 mgO2/g/h). These results show that the tests were valid and that the sample of activated sludge employed was sensitive to inhibition.
- Other: no data - Reported statistics and error estimates:
- The EC50 and 95% confidence limits of the test and reference substances were calculated using the SAFEstat curvefit program. Since the permitted maximum difference for specific respiration rates in control beakers established at the beginning and end of the test period was 15%, this value was used to define the criteria for biologically significant levels of inhibition in mixtures containing the test substance.
Any other information on results incl. tables
Table 1. Temperature, pH and respiration rate measurements.
Test mixture |
Initial Temperature | Final Temperature | Initial pH | Final pH | Specific respiration rate mgO2/g/h | % inhibition |
Control (1) |
19.9 |
19.8 | 7.6 | 8.1 | 25.0 | - |
Control (2) |
19.7 |
19.7 | 7.6 | 8.1 | 24.6 | - |
Test substance |
|
|||||
500 |
19.8 | 19.6 | 7.5 | 8.1 | 19.0 | 23 |
1000 |
19.8 | 19.7 | 7.5 | 8.1 | 18.3 | 26 |
2000 |
19.9 | 19.6 | 7.4 | 7.9 | 16.3 | 34 |
4000 |
19.8 | 19.6 | 7.3 | 7.8 | 14.5 | 42 |
8000 |
19.8 | 19.7 | 7.1 | 7.6 | 13.0 | 47 |
Positive control |
|
|||||
3 |
19.8 | 19.7 | 7.6 | 8.2 | 17.6 | 29 |
10 |
19.8 | 19.7 | 7.5 | 8.1 | 12.5 | 50 |
32 |
19.7 | 19.6 | 7.6 | 8.1 | 6.0 | 76 |
Applicant's summary and conclusion
- Conclusions:
- Concentration-related inhibition of respiration rates occurred at each test substance concentration in the definitive test. The highest tested concentration of the test substance in the definitive test (8000 mg/L) caused 47% inhibition. The EC50 of the test substance was calculated from the results of the definitive test to be 960 mg/L (95% confidence limits, 6820 - 16,000 mg/L). The EC20 value was calculated to be 356 mg/L (95% confidence limits, 171 - 614 mg/L). The highest test concentration tested (8000 mg/L) caused 47% inhibition.
- Executive summary:
Not applicable
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