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EC number: 203-104-6 | CAS number: 103-36-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
The test item is not an acute toxic substance via the oral route.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Principles of method if other than guideline:
- No detailed information is available on the test method.
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
- Species:
- guinea pig
- Strain:
- not specified
- Sex:
- male/female
- Route of administration:
- oral: gavage
- Vehicle:
- other: sunflower-seed oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 20-45%
- Amount of vehicle (if gavage):0.2-0.5 mL/kg bw
- Justification for choice of vehicle: because of low solubility of the substance - Doses:
- 20-45% solution in the vehicle
- No. of animals per sex per dose:
- 6 animals/sex/dose
- Control animals:
- not specified
- Details on study design:
- Duration of observation period following administration: 15 days
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 4 000 mg/kg bw
- Based on:
- test mat.
- Interpretation of results:
- other: CLP/EU GHS criteria not met, no classification required according to Regulation (EC) No. 1272/2008
- Conclusions:
- The test substance had a LD50 of 4000 mg/kg bw given as a single oral dose.
- Executive summary:
The study has been performed in order to describe the toxic propreties of the test substance in guinea pigs after a single dose.
Each group of 6 males and 6 females of guinea pigs were administered the substance through a stomach tube. The substance was administered in the form of a 20 - 45% solution in sunflower-seed oil (0.02 - 0.05 mL per kg bw) and a single dose was given.
The animals were observed within 15 days.
No differences in the sex sensitivity of these animals were observed. The LD50 was found to be 4000 mg/kg bw, and so the test substance can be referred to as a low-toxic compound.
According to the regulation 1272/2008 on CLP, the substance does not need to be classified as an acute toxic.
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Principles of method if other than guideline:
- No detailed information is available on the test method.
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
- Species:
- mouse
- Strain:
- other: White/CD-1
- Sex:
- male/female
- Route of administration:
- oral: gavage
- Vehicle:
- other: sunflower-seed oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 20-45%
- Amount of vehicle (if gavage):0.2-0.5 mL/kg bw
- Justification for choice of vehicle: because of low solubility of the substance - Doses:
- 20-45% solution in the vehicle
- No. of animals per sex per dose:
- 6 animals/sex/dose
- Control animals:
- not specified
- Details on study design:
- Duration of observation period following administration: 15 days
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 4 000 mg/kg bw
- Based on:
- test mat.
- Interpretation of results:
- other: CLP/EU GHS criteria not met, no classification required according to Regulation (EC) No. 1272/2008
- Conclusions:
- The test substance had a LD50 of 4000 mg/kg bw given as a single oral dose.
- Executive summary:
The study has been performed to assess the toxic properties of the test substance in mice after a single dose.
Each group of 6 males and 6 females of White/CD-1 mice were administered the substance through a stomach tube. The substance was administered in the form of a 20 - 45% solution in sunflower-seed oil (0.02 - 0.05 mL per kg bw) and a single dose was given.
The animals were observed within 15 days.
No differences in the sex sensitivity of these animals were observed. The LD50 was found to be 4000 mg/kg bw, and so the test substance can be referred to as a low-toxic compound.
According to the regulation 1272/2008 on CLP, the substance does not need to be classified as an acute toxic.
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Principles of method if other than guideline:
- No detailed information is available on the test method.
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
- Species:
- rat
- Strain:
- not specified
- Sex:
- not specified
- Route of administration:
- oral: unspecified
- Vehicle:
- not specified
- Doses:
- 2.56, 3.20, 4.0 and 5.0 g/kg bw
- No. of animals per sex per dose:
- 10
- Control animals:
- not specified
- Details on study design:
- There are no further details available.
- Sex:
- not specified
- Dose descriptor:
- LD50
- Effect level:
- 7 800 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 7 410 - <= 8 190
- Mortality:
- 2.56 g/kg bw: 0/10
3.20 g/kg bw: 2/10
4.0 g/kg bw: 7/10
5.0 g/kg bw: 9/10 - Clinical signs:
- other: The animals which died experienced sluggishness prior to death.
- Interpretation of results:
- other: CLP/EU GHS criteria not met, no classification required according to Regulation (EC) No. 1272/2008
- Conclusions:
- The oral LD50 was 7.8 g/kg with 95% confidence limits 7.41 and 8.19 g/kg. The substance does not need to be classified according to Regulation (EC) No. 1272/2008 (CLP).
- Executive summary:
In the current study a standard acute method was used to determine the acute oral toxicity of the test item. 10 animals/dose at 2.56, 3.20, 4.0 and 5.0 g test substance/kg bw were dosed once and observed for 14 days. The respective mortalities were 0, 2, 7 and 9. The animals which died experienced sluggishness prior to death.
The oral LD50 was found to be 7.8 g/kg (95% confidence limits: 7.41 and 8.19 g/kg).
This is above the cutoff value of 2000 mg/kg bw of the CLP Regulation and therefore, the substance does not need to be classified.
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Principles of method if other than guideline:
- No detailed information is available on the test method.
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
- Species:
- rat
- Strain:
- other: Albino
- Sex:
- male/female
- Route of administration:
- oral: gavage
- Vehicle:
- other: sunflower-seed oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 20-45%
- Amount of vehicle (if gavage):0.2-0.5 mL/kg bw
- Justification for choice of vehicle: because of low solubility of the substance - Doses:
- 20-45% solution in the vehicle
- No. of animals per sex per dose:
- 6 animals/sex/dose
- Control animals:
- not specified
- Details on study design:
- Duration of observation period following administration: 15 days
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 4 000 mg/kg bw
- Based on:
- test mat.
- Interpretation of results:
- other: CLP/EU GHS criteria not met, no classification required according to Regulation (EC) No. 1272/2008
- Conclusions:
- The test substance had a LD50 of 4000 mg/kg bw in rats given as a single oral dose.
- Executive summary:
The study has been performed to assess the toxic properties of the test substance in rats after 1 oral dose.
Each group of 6 males and 6 females of albino rats were administered the substance through a stomach tube. The substance was administered in the form of a 20 - 45% solution in sunflower-seed oil (0.02 - 0.05 mL per kg bw) and a single dose was given.
The animals were observed for 15 days.
No differences in the sex sensitivity of these animals were observed. The LD50 was found to be 4000 mg/kg bw, and so the test substance can be referred to as a low-toxic compound.
According to the regulation 1272/2008 on CLP, the substance does not need to be classified as an acute toxic.
- Endpoint:
- acute toxicity: oral
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- Read-across from benzyl cinnamate to ethyl cinnamate is considered justified based on strong similarities with regard to chemical structure and metabolic pathways. A full read-across justification including comparison of toxicological profiles is included in section 13 of the IUCLID dossier.
- Reason / purpose for cross-reference:
- read-across source
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 2 426 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 1 936 - <= 3 023
- Remarks on result:
- other: Corrected for MW
- Interpretation of results:
- other: CLP/EU GHS criteria not met, no classification required according to Regulation (EC) No. 1272/2008
- Conclusions:
- Read-across was done from an appropriate source substance. Based on the result, and on the structural, chemical and toxicological similarities between the source and the target substance, the LD50 for the target substance was calculated to be 2426 mg/kg bw with 19/20 confidence limits of 1936 to 3023 mg/kg bw.
- Executive summary:
Read across was done from an appropriate source substance. The oral LD50 of the source substance was determined in rats in an oral (gavage) acute toxicity test. No OECD guideline was followed and the study was not GLP.
The rats were fed 2.0, 2.25, 3.0 or 5.0 g/kg of the source substance dissolved in corn oil, via a rigid stomach tube. 10 animals per dose were used. Following the administration of each dose level the animals were observed for 14 days for signs of toxicity.
8/10 animals dosed with 5 g/kg died within 24 hours post-dosing, the remaining 2 showed a normal increase in body weight at the end of the 14 days observation period. All surviving animals ate well and gained body weight in a normal way.
Animals dosed with 5 g/kg bw showed CNS effects 18 hours post-dosing. Animals dosed at the three lower levels did not show any symptoms prior to death and behaved as normal laboratory animals.
The oral LD50 for the source substance was calculated to be 3.28 g/kg bw with 19/20 confidence limit of 2.62 to 4.1 g/kg bw.
Based on the result, and on the structural, chemical and toxicological similarities between source and target substance, the LD50 for the target substance was calculated to be 2426 mg/kg bw with 19/20 confidence limit of 1936 to 3023 mg/kg bw.
As the LD50 > 2,000 mg/kg bw, the test item is not to be classified as acute toxic according to the CLP regulation.
Referenceopen allclose all
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 2 426 mg/kg bw
Acute toxicity: via inhalation route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Principles of method if other than guideline:
- No data on the test method available.
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- yes
- Species:
- rabbit
- Strain:
- not specified
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- No data available.
- Type of coverage:
- not specified
- Vehicle:
- not specified
- Doses:
- 5.00 g/kg bw
- No. of animals per sex per dose:
- 10
- Control animals:
- not specified
- Sex:
- not specified
- Dose descriptor:
- LD50
- Effect level:
- > 5 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- 0/10
- Clinical signs:
- other: none reported
- Interpretation of results:
- other: CLP/EU GHS criteria not met, no classification required according to Regulation (EC) No. 1272/2008
- Conclusions:
- The dermal LD50 is > 5000 mg/kg bw and therefore, the substance does not need to be classified according to CLP regulation.
- Executive summary:
In the current study a standard acute method was used to determine the acute dermal toxicity of the test item, however, no data are available concerning type and duration of dermal coverage. 10 animals were dosed once and observed for 14 days.
At a dose of 5.00 g/kg bw no animal died and no clinical signs were reported.
It can be concluded that the LD50 is > 5000 mg/kg bw as at this dose 0 out of 10 animals died. This is above the cutoff value of 2000 mg/kg bw of the CLP Regulation and therefore, the substance does not need to be classified.
- Endpoint:
- acute toxicity: dermal
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- Read-across from benzyl cinnamate to ethyl cinnamate is considered justified based on strong similarities with regard to chemical structure and metabolic pathways. A full read-across justification including comparison of toxicological profiles is included in section 13 of the IUCLID dossier.
- Reason / purpose for cross-reference:
- read-across source
- Sex:
- not specified
- Dose descriptor:
- LD50
- Effect level:
- > 2 217 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: Corrected for MW
- Interpretation of results:
- other: CLP/EU GHS criteria not met, no classification required according to Regulation (EC) No. 1272/2008
- Conclusions:
- Read-across was done from an appropriate source substance. Based on the result, and on the structural, chemical and toxicological similarities between the source and target substance, the LD50 for the target substance was calculated to be > 2217 mg/kg bw.
- Executive summary:
Read-across was done from an appropriate source substance. In the acute dermal toxicity study the source substance was applied once to the clipped backs of rabbits, on both abraded and intact clipped skin areas. The dose levels were 1, 2 or 3 g/kg bw. The animals were covered with a rubber sleeve which fit snuggle around the animal. The animals were placed in an animal holder and exposed for a period of 24 hours.
After the exposure period of 24 hours, the rubber sleeves were removed and the skin reactions were recorded. Each animal was thoroughly wiped down and returned to its own metabolism cage to be observed for the following 14 days.
Examination of the treated backs after 24 hours showed moderate erythema but no edema or eschar formation and 24 hours later the treated backs showed a decrease in the degree of erythema. 48 hours after exposure the treated backs were normal.
The animals consumed their daily ration, gained weight and behaved normal. No animals died during the test and the animals did not show any clinical signs of toxicity, nor any significant differences between their initial and final haematogram. From these data an LD50 > 3000 mg/kg bw for the source substance could be deduced.
Based on the result, and on the structural, chemical and toxicological similarities between the source and target substance, and taking into account the difference in molecular weight range between both substances, the LD50 for the target substance was calculated to be > 2217 mg/kg bw.
This is above the cutoff value of 2000 mg/kg bw of the CLP Regulation and therefore, the substance does not need to be classified.
Referenceopen allclose all
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 2 217 mg/kg bw
Additional information
Acute oral toxicity
For the acute oral toxicity endpoint there are 3 studies available, 2 studies on the test substance itself and 1 study performed with an appropriate source substance for read across.
In the first study on the test substance itself (1973), 10 animals were dosed once and observed for 14 days. The oral LD50 was found to be 7.8 g/kg bw with 95% confidence limits of 7.41 and 8.19 g/kg bw.
The second study (1973) comprises data on 3 different species. The toxic properties of the substance were assessed in albino rats, White/CD-1 mice and guinea pigs.
Groups of 6 male and 6 female animals were fed through a stomach tube. The substance was administered in the form of a 20 - 45% solution in sunflower-seed oil (0.02 - 0.05 mL per kg bw) and a single dose was given. The animals were observed for 15 days.
No differences in the sex sensitivity of these animals were observed and the LD50 was found to be 4000 mg/kg bw for all species.
The last study was done on an apprpriate read-across source substance. The oral LD50 of the source substance was determined in rats in an oral (gavage) acute toxicity test. Rats were fed 2.0, 2.25, 3.0 or 5.0 g/kg bw of the source substance dissolved in corn oil via a rigid stomach tube. 10 animals wer used per dose and were observed for 14 days.
The oral LD50 for the source substance was calculated to be 3.28 g/kg bw with 19/20 confidence limit of 2.62 to 4.1 g/kg bw.
Based on the result, and on the structural, chemical and toxicological similarities between the source and the target substance, and taking into account the difference in molecular weight between the source and target substance, the LD50 for the target substance was calculated to be 2426 mg/kg bw with 19/20 confidence limit of 1936 to 3023 mg/kg bw.
Taken together and being conservative, the LD50 of the test substance is considered to be 2426 mg/kg bw, and the test item is not to be classified as acute toxic according to the CLP regulation.
Acute dermal toxicity
For the acute dermal toxicity endpoint there are 2 studies available, 1 on the test substance itself and 1 on an appropriate source substance for read across.
In the study on the substance itself (1973) the acute dermal toxicity was tested in 10 animals which were dosed once and observed for 14 days.
At a dose of 5.00 g/kg bw no animal died and no clinical signs were reported. It can be concluded that the LD50 is > 5000 mg/kg bw as at this dose 0 out of 10 animals died.
In the study on the source substance the acute dermal toxicity was assessed by applying the source substance once to the clipped backs of rabbits, on both abraded and intact clipped skin areas. The doses used were 1, 2 or 3g/kg bw. The animals were covered with a rubber sleeve and in an animal holder exposed for 24 hours.
After exposure, the rubber sleeves were removed and the skin reactions were recorded. Each animal was thoroughly wiped down and returned to its own metabolism cage to be observed for the following 14 days.
No animals died during the test and the animals did not show any toxicity signs, and so the LD50 > 3000 mg/kg bw for the source substance.
Based on the result, and on the structural, chemical and toxicological similarities between the source and target substance, and taking into account the difference in molecular weight between the source and target substance, the LD50 for the target substance was calculated to be > 2217 mg/kg bw.
Taken together and being conservative, the LD50 of the test substance is considered to be > 2217 mg/kg bw, and the test item is not to be classified as acute toxic according to the CLP regulation.
Justification for classification or non-classification
Acute oral toxicity
According to the EU Regulation No. 1272/2008 on the Classification, Labelling and Packaging of Substances and Mixtures (CLP) the substance is not considered to be classified as acute toxic because the LD50 is 2426 mg/kg bw, which is > 2000 mg/kg bw (Table 3.1.1).
Acute dermal toxicity
According to the EU Regulation No. 1272/2008 on the Classification, Labelling and Packaging of Substances and Mixtures (CLP) the substance is not considered to be classified as acute toxic because the LD50 is 2217 g/kg bw, which is > 2000 mg/kg bw (Table 3.1.1).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.