A mixture of: 3,3'-dicyclohexyl-1,1'-methylenebis(4,1-phenylene)diurea; 3-cyclohexyl-1-(4-(4-(3-octadecylureido)benzyl)phenyl)urea; 3,3'-dioctadecyl-1,1'-methylenebis(4,1-phenylene)diurea

Short-term toxicity to aquatic invertebrates

Description of key information

The 24 -h EC50 in Daphnia magna is higher than the limit of solubility in test medium at a nominal loading rate of >1000 mg/L.

Additional information

The toxicity to aquatic invertebrates was examined in a study according to OECD TG 202 and in compliance with GLP criteria. Groups of daphnids (2 replicates of 10) were exposed to test item concentrations of 0 (control), 1000 mg/L filtered (i.e. saturated solution), and 1000 mg/L unfiltered (i.e. supersaturated solution) for 24 hours under static conditions. No test substance analysis was performed. Mobility of the test animals was recorded after 24 hours exposure. After the exposure period no immobility of daphnids was observed in any of the test vessels. Based on these findings, the 24 -h EC50 in Daphnia magna is higher than the limit of solubility in test medium at a nominal loading rate of >1000 mg/L.

Additional note: 

The 24-h EC50 value as determined in the Daphnia acute toxicity test is in line with the OECD TG 202 version 1984. Although at present a 48-h EC50 value would be required for assessment, it was decided that no new study (acute or chronic) will be performed due to the absence of a suitable analytical method (see expert statement on hydrolysis). Furthermore, chronic effects are not expected to occur due the limited bioavailability and potential for bioaccumulation of the substance and the complete absence of effects in the available aquatic toxicity dataset. Altogether, the currently available information is considered to be adequate for assessment.