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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The acute toxicity of the test item to the freshwater invertebrate Daphnia magna, investigated using a method designed to be compatible with OECD 202 and EU Method C.2, gave a 48-hour EL50 value of greater than 100 mg/L. The No observed Effect Loading rate was 100 mg/L.

Key value for chemical safety assessment

Additional information

GUIDELINE

A study was performed to assess the acute toxicity of the test item to Daphnia magna. The method was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphnia sp., Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No 440/2008.

METHODS

Due to the low aqueous solubility and complex nature of the test item, for the purposes of the test, the test medium was prepared as a Water Accomodated Fraction (WAF).

Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) were exposed to a Water Accomodated Fraction (WAF) of the test item at single nominal loading rate of 100 mg/L for 48 hours at a temperature of approximately 20°C under static test conditions. The number of immobilised Daphnia and any adverse reactions to exposure were recorded after 24 and 48 hours.

RESULTS

Chemical analysis of the 100 mg/L loading rate test preparations at 0 and 48 hours showed measured test concentrations of 26 and 27 mg/L respectively.

Given that the toxicity cannot be attributed to a single component or a mixture of components, but to the test item as a whole, the results were based on nominal loading rates only.

Exposure of Daphnia magna to the test item gave EL50 values of greater than 100 mg/L loading rate WAF. The No Observed Effect Loading Rate was 100 mg/L loading rate WAF.

It was considered unnecessary and unrealistic to test at loading rates in excess pf 100 mg/L loading rate WAF.