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EC number: 252-525-1 | CAS number: 35355-77-2 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 15880:2.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1975
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 975
- Report date:
- 1975
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: The method given in the "Hazardous Substance Regulations" unter the U.S. Federal Hazardous Substances Labelling Act Sect. 191.11 (February 1965)
- GLP compliance:
- no
Test material
- Reference substance name:
- Manganese, 3-hydroxy-4-[(1-sulfo-2-naphthalenyl)azo]-2-naphthalenecarboxylic acid complex
- EC Number:
- 252-525-1
- EC Name:
- Manganese, 3-hydroxy-4-[(1-sulfo-2-naphthalenyl)azo]-2-naphthalenecarboxylic acid complex
- Cas Number:
- 35355-77-2
- Molecular formula:
- C21H12MnN2O6S
- IUPAC Name:
- manganese(2+) hydrogen 3-oxido-4-[(E)-(1-sulfonatonaphthalen-2-yl)diazenyl]naphthalene-2-carboxylate
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Physical state: purple powder
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 11 - 16 weeks
- mean weight at study initiation: males 2.57 kg and females 2.60 kg
- Housing: animals were caged singly in an experimental room
- Diet: a commercial irradiated diet (Styles-Oxoid) was fed ad libitum
- Water: sterile filtered water was available at all time
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 ± 1
- Humidity (%): 50-70
- Photoperiod: Animals were exposed to artificial light for 10 hours daily from 08.00 - 18.00 hours
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- other: abraded and shaved
- Vehicle:
- other: polyethylene glucol
- Controls:
- other: untreated skin of the same animals
- Amount / concentration applied:
- TEST MATERIAL
- Amount applied: 10 g of the test item was mixed with 20 mL of 50% solution polyethylene glycol and water to make a solution of 20 mL - Duration of treatment / exposure:
- 24 hours
- Observation period:
- 6 days
- Number of animals:
- 6
- Details on study design:
- TEST SITE
- Area of exposure: 2.5 cm x 2.5 cm
- Application side: back
- % coverage: at least 10% of the total body surface
- Type of wrap if used: aluminium foil secured with "sleek" adhersive tape. The test sides were than enclosed by a 6 wide Coban self adhesive bandage the edges of which were fixed to the skin by stripes.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- other: 24h - 72h
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- other: intact skin
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- other: 24h - 72 h
- Score:
- 0.33
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72 hours
- Remarks on result:
- other: intact skin
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- other: 24h - 72h
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- other: intact skin
- Irritation parameter:
- edema score
- Basis:
- animal #2
- Time point:
- other: 24h - 72h
- Score:
- 0.5
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72 hours
- Remarks on result:
- other: intact skin
- Irritation parameter:
- edema score
- Basis:
- animal #3
- Time point:
- other: 24h - 72h
- Score:
- 0.5
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72 hours
- Remarks on result:
- other: intact skin
- Irritation parameter:
- edema score
- Basis:
- animal #4
- Time point:
- other: 24h - 72h
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- other: intact skin
- Irritation parameter:
- edema score
- Basis:
- animal #5
- Time point:
- other: 24h - 72h
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- other: intact skin
- Irritation parameter:
- edema score
- Basis:
- animal #6
- Time point:
- other: 24h - 72h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72 hours
- Remarks on result:
- other: intact skin
- Irritant / corrosive response data:
- The test substance ist not irritating to intact skin within 72 hours.
Any other information on results incl. tables
Table 1: Erythema formation
Erythema Formation | |||||
| Intact | Abraded | Total | ||
No | 24h | 72h | 24h | 72h |
|
11 | 0 | 0 | 1 | 0 | |
13 | 0 | 0 | 1 | 0 | |
15 | 0 | 0 | 1 | 0 | |
12 | 0 | 0 | 1 | 0 | |
14 | 0 | 0 | 1 | 0 | |
16 | 0 | 0 | 1 | 0 | |
Mean | 0.00 | 0.00 | 1.00 | 0.00 | 1.00 |
Table 2: Oedema formation
Oedema Formation | |||||
| Intact | Abraded | Total | ||
No | 24h | 72h | 24h | 72h |
|
11 | 0 | 0 | 2 | 0 | |
13 | 1 | 0 | 2 | 0 | |
15 | 1 | 0 | 2 | 1 | |
12 | 0 | 0 | 2 | 0 | |
14 | 0 | 0 | 2 | 0 | |
16 | 2 | 0 | 3 | 1 | |
Mean | 0.67 | 0.00 | 2.17 | 0.33 | 3.17 |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Because of the findings regarding the intact skin, the test substance is not considered irritating to the skin.
- Executive summary:
Groups of six New Zealand White rabbits (4 females, 2 males) were dermally exposed to the test item. Twenty-four hours prior to the dermal application, the backs of the rabbits were shaved over an area consisting of at least 10% of the total body surface. Two test sites lateral to the mid line of the back were used. Immediately before the application of the test compound, the right hand site was abraded, the left hand site remained intact. 10 g of the test item was mixed with 20 mL of a 50% solution of polyethylene glycol and water to make a solution of 20 mL. 1.5 mL was applied to each test site on a 2.5 cm gauze pad. The test sites were covered with an occlusive dressing for 24 hours. No erythema effect was observed on the intact skin sites after application of the test compound. On the abraded sites 6/6 animals showed a slight erythema effect, which was fully reversible within 72 hours. Slight to moderate edema was seen on 3/6 intact sites and 6/6 abraded sites, 24 hours after application of the compound. Verly slight edema was still present on 2/6 abraded sites at 72 hours, but had returned to normal by day 6. The observed edema effect to the intact skin was also fully reversible within 72 hours. The observed mean edema and erythema score for the intact skin were 0.33 and 0, repectively.
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