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EC number: 274-503-0 | CAS number: 70247-74-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 31 Jul 1943- 06 August 1974
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 974
- Report date:
- 1974
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: "Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics" (1959) of the US Association of Food and Drug Officials (AFDO).
- Deviations:
- no
- GLP compliance:
- no
Test material
- Reference substance name:
- Sodium bis[N-(2-chlorophenyl)-2-[[2-hydroxy-5-(N-methylsulphamoyl)phenyl]azo]-3-oxobutyramidato(2-)]cobaltate(1-)
- EC Number:
- 274-503-0
- EC Name:
- Sodium bis[N-(2-chlorophenyl)-2-[[2-hydroxy-5-(N-methylsulphamoyl)phenyl]azo]-3-oxobutyramidato(2-)]cobaltate(1-)
- Cas Number:
- 70247-74-4
- Molecular formula:
- C34H30Cl2CoN8O10S2.Na
- IUPAC Name:
- sodium bis[N-(2-chlorophenyl)-2-[[2-hydroxy-5-(N-methylsulphamoyl)phenyl]azo]-3-oxobutyramidato(2-)]cobaltate(1-)
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- None
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- other: Russian breed
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Weight at study initiation:1.8 to 2.2 kg
- Housing: kept separately in metal cages (overall dimensions 47x32x34cm)
- Diet: standard diet of Nafag ad libitum
- Water: ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 18 ± 1
- Humidity (%): 55 ± 5
- Photoperiod (hrs dark / hrs light): 14 hours light/day
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not specified
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 g - Duration of treatment / exposure:
- 1 minute
- Observation period (in vivo):
- 1, 2,3 and 7 days
- Number of animals or in vitro replicates:
- 6 rabbits (3 males and 3 females)
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done):The eyes of 3 animals were rinsed 1 minute after application with physiologic saline.
SCORING SYSTEM:
0-10 = minimal
11 - 25 = slight
26 - 55 = moderate
56 - 85 = marked
86 - 110 = extreme
TOOL USED TO ASSESS SCORE: slit lamp
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Remarks:
- unrinsed and rinsed eyes
- Basis:
- mean
- Remarks:
- 6 animal
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- iris score
- Remarks:
- unrinsed and rinsed eyes
- Basis:
- mean
- Remarks:
- 6 animal
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- conjunctivae score
- Remarks:
- unrinsed and rinsed eyes
- Basis:
- mean
- Remarks:
- 6 animal
- Time point:
- 24 h
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- fully reversible within: 72 h
- Remarks on result:
- positive indication of irritation
- Irritation parameter:
- conjunctivae score
- Remarks:
- unrinsed and rinsed eyes
- Basis:
- mean
- Time point:
- 48 h
- Remarks on result:
- not measured/tested
- Irritation parameter:
- conjunctivae score
- Remarks:
- unrinsed and rinsed eyes
- Basis:
- mean
- Remarks:
- 6 animal
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 3
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- chemosis score
- Remarks:
- unrinsed and rinsed eyes
- Basis:
- mean
- Remarks:
- 6 animal
- Time point:
- 24/48/72 h
- Remarks on result:
- not measured/tested
- Other effects:
- None
Any other information on results incl. tables
Preparation FAT 20 035/A was found to be practically non irritant when applied to the rabbit eye mucosa. Minimal transient conjunctival reaction was noted 1 and 6 hours following instillation. After 24 hours all findings were negative in 2/3 unrinsed and 1/3 rinsed eyes.
Calculation of the primary eye irritation index
time after exposure h |
mean reaction score of 3 rabbits |
|||||
unrinsed eyes (A) |
rinsed eyes (B) |
|||||
cornea |
iris |
conjunctiva |
cornea |
iris |
conjunctiva |
|
6 |
0 |
0 |
4.0 |
0 |
0 |
4.7 |
24 |
0 |
0 |
2.0 |
0 |
0 |
2.0 |
72 |
0 |
0 |
0 |
0 |
0 |
0 |
subtotal |
0 |
0 |
6.0 |
0 |
0 |
6.7 |
total |
6.0 |
6.7 |
primary irritation index in unrinsed eyes A = 6.0 :3 = 2.0
primary irritation index in rinsed eyes P. = 6.7 -"3 = 2.2
effect of rinsing R = A / B = 2.0 / 2.2 = 0.9
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- FAT 20035/A was found to be practically non irritant when applied to the rabbit eye mucosa.
- Executive summary:
A study was performed to determine the eye irritation potential of FAT 20035/A on rabbits. Test method was followed as described in the "Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics" (1959) of the US Association of Food and Drug Officials (AFDO). The substance was tested on 6 rabbits of the Russian breed. Amounts of 0.l g of the test substance were introduced into the conjunctival sac of the left eye with a spatula . After application, the eyelids were held open for a few seconds. The right eye was left untreated and served as a control. The eyes of 3 animals were rinsed 1 minute after application with physiologic saline. The reactions were appraised with a slit lamp after 1 and 6 hours; 1; 2; 3 and 7 days on the basis of "Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics" (1959) of the AFDO. Preparation FAT 20035/A was found to be practically non-irritant when applied to the rabbit eye mucosa. Minimal transient conjunctival reaction was noted 1 and 6 hours following instillation. After 24 hours all findings were negative in 2/3 unrinsed and 1/3 rinsed eyes. It can be concluded that FAT 20035/A is practically non irritant when applied to the rabbit eye mucosa.
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