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EC number: 942-520-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- September 2019 to January 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- July 22, 2010
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test itm was directly weighed into the test vessel and dstilled water was added. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
Shenyang North Sewage Treatment Plant 1 (aeration tank)
- Preparation of inoculum for exposure: Coarse particles were remoed by settling for 15 minutes and by decanting the upper layer of finer solids. The sludge was centrifuged for 5 minutes at ca. 2500 rmp to produce a clear supernatant and pellet of sewage solids. The supernatant liquid was discarded and the sludge was re-suspended in chlorine-free tap water by shaking. The wash-water was removed by re-centrifuging and discarding again. After re-suspension of the sludge pellet in chlorine-free tap water, the solids concentration was determined by drying 4 replicates of 10 ml suspended sludge at 105°C for 1 hour and 48 minutes. From the measured results, the sludge was diluted further in chlorine-free tap water to obtain the required sludge solid concentration of 3.0 g/L. The activated sludge was fed with 50 ml/L syntheic sewage feed and continuously aerated at the test temperature (20.0-21.4 °C) overnight prior to use in the test. Before the inoculum was used in the test, the concentration of the sludge was determined again by the drying method, and the concentration was adjusted to 3.0 g/L. - Test type:
- static
- Water media type:
- other: distilled water
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20.1-21.4 °C
- pH:
- 7.01-7.73
- Dissolved oxygen:
- 60-70 %
- Nominal and measured concentrations:
- nominal
- Details on test conditions:
- TEST SYSTEM
- Aeration: clean oil-free air, flow rate 0.5 - 1 L/min
- No. of vessels per concentration (replicates): 1 (10, 100, 300 mg/L and reference substance) 3 (1000 mg/L)
- No. of vessels per control (replicates): 4 per concentration
- No. of vessels per abiotic control (replicates): 3
- Sludge concentration (weight of dry solids per volume): 3.0 g/L
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): oxygen consumption
TEST CONCENTRATIONS
- Test concentrations: 0, 10, 100, 300, 1000 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (3,5-DCP)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- No oxygen uptake in the abiotic control. In the test item mixture, the average percentage inhibition was - 1.0% at 1000 mg/L (no significant inhibitory effect)
- Blank controls oxygen uptake rate: 23.6 mg(g*h) oxygen
- Coefficient of variation of oxygen uptake rate in control replicates: 8.1 % - Results with reference substance (positive control):
- - Results with reference substance valid: yes
- Relevant effect levels: EC50= 8.8 mg/l - Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 (3 h) of the test item for total respiratory inhibition to activated sludge was greater than 1000 mg/L.
- Executive summary:
To investigate the toxicity to microorganisms an activated sludge respiration inhibition test was conducted. Activated sludge was incubated with test item at 4 different concentrations of up to 1000 mg/L. The rate of respiration of each incubation mixture was determined after the 3 hours exposure. Based on the results, there was no oxygen uptake in the abiotic control. In the test item mixture, the average percentage inhibition was -1.0 % at the concentration of 1000 mg/L. Therefore, the EC50 (3h) of the test item for total respiratory inhibition in activated sludge is greater than 1000 mg/L under the conditions of the study.
Reference
Description of key information
To investigate the toxicity of Guerbet alcohols, C24 -26, branched and cyclic to microorganisms an activated sludge respiration inhibition test was conducted. Activated sludge was incubated with the test item at 4 different concentrations of up to 1000 mg/L. The rate of respiration of each incubation mixture was determined after the 3 hours exposure. Based on the results, there was no oxygen uptake in the abiotic control. In the test item mixture, the average percentage inhibition was -1.0 % at the concentration of 1000 mg/L. Therefore, the EC50 (3h) of the test item for total respiratory inhibition in activated sludge is greater than 1000 mg/L under the conditions of the study.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
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