Registration Dossier
Registration Dossier
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A mixture of isomers of: 1,1'-[(3,5(or 2,4 or 4,6 or 2,6)-dihydroxy-o(or m or p)-phenylene)bis(azo-meta-phenyleneazo{1-[3-(dimethylamino)propyl]-1,2-dihydro-6-hydroxy-4-methyl-2-oxopyridine-5,3-diyl})]dipyridinium-dichloride-dihydrochloride; 1-(1-[3-(dimethylamino)propyl]-5-{3-[x-(4-{1-[3-(dimethylamino)propyl]-1,6-dihydro-2-hydroxy-4-methyl-6-oxo-5-pyridinio-3-pyridylazo}phenylazo)-2,4(or 2,6 or 3,5 or 4,6)-dihydroxyphenylazo]phenylazo}-1,2-dihydro-6-hydroxy-4-methyl-2-oxo-3-pyridyl)pyridinium-dichloride-dihydrochloride (where x is variable)
EC number: 404-540-1 | CAS number: 159405-95-5 BRAUN HM 2763; BROWN HM 2763; BRUN HM 2763; BRUNO HM 2763
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
Description of key information
Nontoxic to earthworms up to a concentration in the soil (dry weight basis) of 1000 mg/kg.
LC50 >1000 mg/kg and LC0 ≥ 1000 mg/kg soil.
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 1 000 mg/kg soil dw
Additional information
The toxicity of test substance to earthworms was tested in an artificial soil system according to the OECD guideline 207. This study tested concentrations in the range from 62.5 to 1000 mg/kg. The test article proved to be nontoxic to earthworms up to a concentration in the soil (dry weight basis) of 1000 mg/kg. Therefore, the LC50 was >1000 mg/kg, and the LC0 ≥1000 mg/kg soil.
In comparison to the control group, no statistically significant inhibitory effect on the average body weight of worms was observed at the lowest concentration of 62.5 mg/kg. At test concentrations of 125, 250, 500 and 1000 mg/kg the average loss in body weight was, in comparison to the body weight loss of the control animals, lower to a statistically significant degree.
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