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EC number: 215-863-0 | CAS number: 1435-71-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with analytical monitoring
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-[(o-nitrophenyl)azo]-p-cresol
- EC Number:
- 215-863-0
- EC Name:
- 2-[(o-nitrophenyl)azo]-p-cresol
- Cas Number:
- 1435-71-8
- Molecular formula:
- C13H11N3O3
- IUPAC Name:
- 4-methyl-2-[(2-nitrophenyl)diazenyl]phenol
- Details on test material:
- - Name of test material (as cited in study report): TKA 40136, Monoazo 3113
- Physical state: Red-brownish solid
- Analytical purity: > 95%
- Lot/batch No.: Pa 369
- Storage condition of test material: At room temperature in the dark
- Expiry date: 1997-11-1
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling: During the final test duplicate samples were taken from 0 mg/L, 10 mg/L and the acetone-control for analysis. Sampling frequency: At t= 0 h and at t= 24 h (10 ml from the approx. centre of the test vessels).
- Storage: The samples were stored in a deep-freeze.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Acetone was used as a presolvent to prepare stock solutions.
- Chemical name of vehicle: Acetone
- Concentration of vehicle in test medium: 0.117 mL/L
- Evidence of undissolved material: Yes
Test organisms
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: Carp
- Source: Zodiac, proefacc, "De Haar Vissen", L.U. Wageningen, The Netherlands
- Length at study initiation: Range-finding test: 2.91 ± 0.26 cm, final study: 2.75 ± 0.34 cm
- Weight at study initiation: Range-finding test: 0.74 ± 0.17 g , final study: 0.76 ± 0.21 g
- Feeding during test: No feeding 24 hours prior to the test and during the total test period.
ACCLIMATION
- Acclimation period: At least 12 days
- Type of food: Daily feeding with Trouvit and Artemia
- Mortality: < 5%
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 250 mg/L CaCO3
- Test temperature:
- 20.2-20.6 °C
- pH:
- 8.1
- Dissolved oxygen:
- 7.1-9.6 mg/L
- Nominal and measured concentrations:
- Nominal: 10 mg/L
Measured:
Spectrophotometric method: 0.58-8.7 mg/L
HPLC method: 1.3-6.3 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 8 liter glass vessels
- Renewal rate of test solution: Every 24 h
- No. of organisms per vessel: 7
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- No. of vessels per vehicle control: 1
- Biomass loading rate: 0.89 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: Daily
OTHER TEST CONDITIONS
- Photoperiod: 16h daily
EFFECT PARAMETERS MEASURED: Mortality
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.1, 1.0 and 10 mg/L
- Results used to determine the conditions for the definitive study: No mortality observed in the highest concentration - Reference substance (positive control):
- yes
- Remarks:
- Pentachlorophenol
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- >= 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: 0
- Any observations that might cause a difference between measured and nominal values: Precipitation led to a significant decrease of the desired concentration.
- Effect concentrations exceeding solubility of substance in test medium: Yes - Results with reference substance (positive control):
- - LC50 = 0.26 mg/L
Applicant's summary and conclusion
- Conclusions:
- The measured concentrations were clearly lower than the nominal concentration and the LC50 value exceeds the water solubility of the tested substance.
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