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EC number: 273-086-2 | CAS number: 68937-75-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Remarks:
- Pseudomonas putida, respiration inhibition, 18 h
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 Mar - 16 Mar 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Basic data given (comparable to guideline)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: ISO 10712
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A saturated solution containing 680 mg prod./L and an oversaturated solution containing 1360 mg/L were prepared using sterile Milli-Q water which was mixed with an magnetic stirrer for 10 min. The pH was adjusted to 6.8 - 7.2 with NaOH.
- Controls: 10 with inoculum without test substance (series B) to determine turbidity and for each test concentration 1 control without inoculum to determine occuring colouration - Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: obtained from RIVM, Bilthoven, Netherlands
- Method of cultivation: kept in agar plant tubes with a nutrient stock solution, incubated at 25 °C
- Preparation of inoculum for exposure: 7 days old stock cultures were inoculated in a fluid nutrient medium in Erlenmeyer flasks for 16 - 20 h at 25 °C. Subsequently the extinction of the monochromatic radiation was measured at 436 nm in a 10 mm layer of the bacterial suspension by photoelectric measurement. On basis of the measured values the turbidity was adjusted with sterile saline to the value of the Formazin standard suspension (TU/F/436 nm = 10) with which the spectrophotometer was calibrated. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 18 h
- pH:
- 6.8 - 7.2, adjusted with NaOH
- Nominal and measured concentrations:
- nominal: 0.266, 0.531, 1.063, 2.125, 4.25, 8.50, 17.00, 34.00, 68.00, 136.00, 272.00, 544.00, 1088.00 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 300 mL Erlenmeyer flasks stoppered with aluminum caps containing 100 mL test medium
- No. of vessels per concentration (replicates): 3 + 1 control (please refer to "details of test solution" above)
- No. of vessels per control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: sterile Milli-Q water (Milipore Corp., Bedford, USA)
OTHER TEST CONDITIONS
- Adjustment of pH: adjusted to 6.8 - 7.2 with NaOH solution
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : turbidity after 16 -20 h
- Reference substance (positive control):
- yes
- Remarks:
- Methanol
- Duration:
- 18 h
- Dose descriptor:
- EC10
- Effect conc.:
- 912 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: mean extinction value
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Reported statistics and error estimates:
- The toxicity threshold (TT) is determined graphically. The mean extinction values of each dilution step were plotted against the logarithm of the mean values of the test substance concentration.
- Endpoint:
- toxicity to microorganisms
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to the category justification provided in IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 18 h
- Dose descriptor:
- EC10
- Effect conc.:
- 912 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: mean extinction value
- Remarks on result:
- other: Source: CAS 124-07-2
Referenceopen allclose all
Table 1: Extinction of the test substances solutions after 16 - 20 h
Concentration [mg prod./L] |
Extinction at 436 nm |
||||
Replicate I |
Replicate II |
Replicate III |
Arithm. mean of replicates |
Replicate without inoculum |
|
0.226 |
0.490 |
0.500 |
0.470 |
0.487 |
0.000 |
0.531 |
0.471 |
0.480 |
0.469 |
0.473 |
0.000 |
1.063 |
0.463 |
0.460 |
0.456 |
0.460 |
0.000 |
2.125 |
0.452 |
0.450 |
0.460 |
0.454 |
0.000 |
4.250 |
0.458 |
0.430 |
0.466 |
0.451 |
0.000 |
8.500 |
0.447 |
0.450 |
0.459 |
0.452 |
0.000 |
17.000 |
0.469 |
0.434 |
0.463 |
0.455 |
0.000 |
34.000 |
0.458 |
0.450 |
0.470 |
0.459 |
0.000 |
68.000 |
0.525 |
0.512 |
0.497 |
0.511 |
0.000 |
136.000 |
0.588 |
0.580 |
0.570 |
0.579 |
0.000 |
272.000 |
0.710 |
0.740 |
0.745 |
0.732 |
0.000 |
544.000 |
0.985 |
0.979 |
0.990 |
0.985 |
0.000 |
1088.000 |
0.198 |
0.212 |
0.220 |
0.210 |
0.000 |
Table 2: Extinction after 16 - 20 h in the reference substance and the blank control
Concentration [mg prod./L] |
Extinction at 436 nm |
|
Reference substance |
Blank controls |
|
4937.500 |
0.488 |
0.449 |
9875.000 |
0.440 |
0.440 |
19750.00 |
0.415 |
0.452 |
39500.00 |
0.270 |
0.459 |
79000.00 |
0.155 |
0.458 |
|
0.449 |
|
0.433 |
||
0.439 |
||
0.440 |
||
0.438 |
||
Arithmetic mean |
0.446 |
With respect to the historical test with the reference substance methanol, it can be concluded that the test conditions were optimal and the results are valid.
Description of key information
EC10 (18 h) = 912 mg/L (ISO 10712); RA CAS 124 -07 -2
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 912 mg/L
Additional information
Good quality read across data from octanoic acid, one of the main constituents of the substance fatty acids, C8 -10, are used to assess the toxicity to microorganisms.
The toxicity of the read across substance octanoic acid towards Pseudomonas putida, is evaluated in a cell multiplication inhibition test (1988). The study was conducted similarly to ISO 10712. The test organism was exposed to a wide range of nominal concentrations (0.266 - 1088.00 mg/L) and an EC10 (18 h) of 912 mg/L (nominal) was determined.
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