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EC number: 284-515-8 | CAS number: 84929-31-7 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Citrus limonum, Rutaceae.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1971
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: It was not indicated if the study was performed according to a guideline or under GLP conditions. However, the methods and results are reported concise but complete.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 971
- Report date:
- 1971
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Lemon, ext.
- EC Number:
- 284-515-8
- EC Name:
- Lemon, ext.
- Cas Number:
- 84929-31-7
- Molecular formula:
- Not applicable (a generic molecular formula cannot be provided for this specific UVCB substance).
- IUPAC Name:
- Essential oil of lemon obtained from the peel of Citrus limonum (Rutaceae) by expression and/or distillation, including cold pressed, distilled, terpenes and essence qualities
- Reference substance name:
- Lemon oil
- IUPAC Name:
- Lemon oil
- Details on test material:
- - Name of test material (as cited in study report):
- Physical state: Liquid
Constituent 1
Constituent 2
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 2.5-3.0 kg
- Housing: Individually
- Diet: Commercial diet
- Water: Ad libitum
ENVIRONMENTAL CONDITIONS
No data
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Area of exposure: Not defined
- % coverage: 10% (240 cm2)
- Type of wrap if used: Rubber sleeve with Webril padding
TEST MATERIAL
- Amount(s) applied: 5 ml/kg
- Constant volume or concentration used: yes - Duration of exposure:
- 24 hours
- Doses:
- 10000 mg/kg bw
- No. of animals per sex per dose:
- 6 (independent of sex)
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: Daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, dermal reactions (scored according to Draize) - Statistics:
- - Horn's method
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 10 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: 1 out of 6 animals died during study
- Mortality:
- 1 out of 6 animals died during study
- Clinical signs:
- other: No toxic signs observed
- Gross pathology:
- No tissue damage observed at termination
- Other findings:
- Skin irritation:
- Erythema score between 2-4 in all animals
- Edema score between 1-3 in 4 animals
- Discoloration noted in 1 animal
- Scaling noted in 5 animals
- Eschar formation noted in 5 animals
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Under the conditions of this study, 1 out of 6 rabbits died after dermal exposure to 10000 mg/kg bw Lemon oil. A LD50 of >10000 mg/kg bw was therefore established and the substance does not need to be classified as acute dermal toxic.
- Executive summary:
The study was performed to determine the acute toxicity potential of Lemon oil. 6 male/female rabbits were treated with 10000 mg/kg bw Lemon oil dermally. Clinical signs and dermal reactions were recorded for 14 days, followed by gross necropsy at the end of the observation period.
Under the conditions of this study, 1 out of 6 rabbits died after dermal exposure to 10000 mg/kg bw Lemon oil. A LD50 of >10000 mg/kg bw was therefore established and the substance does not need to be classified as acute dermal toxic.
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