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EC number: 604-471-9 | CAS number: 14543-49-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in compliance to GLP following OECD-202, Daphnia sp., Acute Immobilization Test, Part 1.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Characterization of the test and reference material used in this study was not conducted following GLP standards; however, a record of custody providing the purity and composition of the test material was provided by the supplier.
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- A transparent yellowish liquid, reported to have a measured purity of 99.65% (comprised of 96.07% trans MOD-1 and 3.58% cis MOD-1 isomers).
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The concentration of MOD-1 in the test solutions was confirmed by analyzing a sample from each replicate fresh test solution on day 0 and each replicate spent test solution on day 2. Aliquots (5 mL) were collected from the control ALDW, 2.72, 4.54 and 7.56 mg/L dose levels and transferred to 4-dram vials containing 5 mL of brine.
The samples were extracted with 1 mL of cyclohexane. For the 12.6, 21.0 and 35.0 mg/L dose levels, aliquots (2 mL) were collected and transferred to 4-dram vials containing 2 mL of brine. The samples were extracted with 2 mL of cyclohexane. All samples were extracted by shaking on a flatbed shaker set on low speed for 30 minutes, then centrifuging for 10 minutes at 2000 rpm. The organic extract was transferred to autosampler vials for analysis by GC/FID.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- 0 (water control), 2.72, 4.54, 7.56, 12.6, 21.0, and 35.0 mg MOD-1/L.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The test organism was the freshwater daphnid, Daphnia magna, from in-house cultures initially obtained from New England Bioassay, Inc., Manchester, Connecticut. Daphniainstars less than 24- hours old from a laboratory-reared culture were used as the test organisms.
Rearing conditions were as follows: illumination (cool-white fluorescent) 2050 ± 350 lux; 16-hour light/8- hour dark photoperiod; temperature 20 ± 2°C. Daphnia were fed a mixed diet of Pseudokirchneriella subcapitata, a freshwater green alga (formerly known as Selenastrum capricornutum) and YCT (yeast, Cerophyll, and trout chow suspension) five times weekly. The day before instars were needed for testing, stock tanks with Daphnia, which have had at least three broods, were removed from the incubator. The instars were separated from adults by gently lifting the screened insert from the 2-L stock tank, releasing instars through the nylon mesh screen while retaining the adult Daphnia. The screened insert containing adult Daphnia was then placed in another stock tank that contained daphnid water. The original solution with instars was poured through a metal sieve into another stock tank. The instars collected on the sieve were discarded, and the original solution was poured back into the initial stock tank. The corresponding screened insert holding adult Daphnia was then put back in place. This procedure was repeated the day the study was set to collect < 24-hour old instars for use in the study.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20-21°C
- pH:
- 7.6 ± 0.1 (7.4-7.7).
- Dissolved oxygen:
- 8.3 ± 0.2 mg/L (8.1 mg/L-8.6 mg/L).
Percent oxygen saturation averaged 93% and remained >= 91% throughout the study - Nominal and measured concentrations:
- Nominal: 0, 2.72, 4.54, 7.56, 12.6, 21.0, and 35.0 mg MOD-1/L.
Measured: <0.2 mg/L (
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 30.8 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 26.3 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 19.1 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- All biological results are expressed in terms of mean measured concentrations of MOD-1.
Summary results of the biological observations are presented in Table 6. Daphnia were observed at 24 and 48 hours of exposure and the primary endpoint of interest in this acute study was immobility. Immobility was observed in 30% (6/20) and 75% (15/20) of the Daphnia at the 30.8 mg/L dose level following 24 and 48 hours of exposure, respectively. In addition, all Daphnia at the 30.8 mg/L dose level were observed to be at the bottom of the vessels following both 24 and 48 hours of exposure. This behavior was not observed in the remaining dose levels or the water control. No immobility was observed in the 19.1 mg/L and below dose levels or in the water control during the conduct of the study. The resulting acute toxicity values for MOD-1 with Daphnia magna were statistically evaluated, where applicable, using mean measured concentrations and are listed below:
• The 24-hour EC50 value was greater than the highest concentration tested of 30.8 mg/L.
• The 48-hour EC50 value was 26.3 mg/L; no reliable 95% confidence interval could be determined.
• The 48-hour NOEC was 19.1 mg/L and was determined based on interpretation of the biological data and the highest exposure level exhibiting no Daphnia immobility.
Applicant's summary and conclusion
- Conclusions:
- The 24-hour EC50 value was greater than the highest concentration tested of 30.8 mg/L.
The 48-hour EC50 value was 26.3 mg/L; no reliable 95% confidence interval could be determined.
The 48-hour NOEC was 19.1 mg/L and was determined based on interpretation of the biological data and the highest exposure level exhibiting no Daphnia immobility.
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