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EC number: 246-836-1 | CAS number: 25321-14-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test procedure according to a national proposed method. (In OECD SIDS: RL1, statement: "Reported in sufficient detail")
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Damaging effects of environmental chemicals in the Daphnia reproduction test as a basis for evaluation of environmental hazard in aquatic systems
- Author:
- Kuehn, R. et al.
- Year:
- 1 988
- Bibliographic source:
- UFOPLAN No. 106 03 052
- Reference Type:
- publication
- Title:
- Results of the harmful effects of water pollutants to Daphnia magna in the 21 day reproduction test
- Author:
- Kuehn, R. et al.
- Year:
- 1 989
- Bibliographic source:
- Water Res. 23 (4), 501-510
- Reference Type:
- secondary source
- Title:
- Dinitrotoluene (isomers mixture), CAS No.: 25321-14-6
- Author:
- OECD SIDS
- Year:
- 2 004
- Bibliographic source:
- UNEP publications
Materials and methods
- Principles of method if other than guideline:
- The study was conducted in accordance with "Proposed preliminary testing method: Prolonged toxicity test on Daphnia magna - determination of NOEC for reproduction rate, mortality, and time of the first appearance of offspring, 21 d - as of 1 January 1984, published as "recommendations of the Federal Environmental Agency for the performance of testing according to § 5, para. 1, No. 3 of the regulations on documents to be submitted and evidence of testing under the chemicals act".
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 2,4-dinitrotoluene
- EC Number:
- 204-450-0
- EC Name:
- 2,4-dinitrotoluene
- Cas Number:
- 121-14-2
- Molecular formula:
- C7H6N2O4
- IUPAC Name:
- 1-methyl-2,4-dinitrobenzene
- Reference substance name:
- 2,6-dinitrotoluene
- EC Number:
- 210-106-0
- EC Name:
- 2,6-dinitrotoluene
- Cas Number:
- 606-20-2
- Molecular formula:
- C7H6N2O4
- IUPAC Name:
- 2-methyl-1,3-dinitrobenzene
- Details on test material:
- - Name of test material (as cited in study report): 2,4-DNT (moistened with 10% water) and 2,6-DNT (moistened with 10% water)
- Analytical purity: no data
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- not specified
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: IRCHA
- Feeding during test
- Food type: tetramin fish feed and actiavted sludge
- Amount: COD = 15-20 mg/L
- Frequency: daily
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- Hardness:
- 2.5 mmol/L Ca + Mg
- Test temperature:
- 25+/-1°C
- pH:
- 8
- Nominal and measured concentrations:
- 0.16-20 mg/L (2,4-Dinitrotoluene), dilution ratio 1:2
0.02-2.5 mg/L (2,6-Dinitrotoluene), dilution ratio 1:2 - Details on test conditions:
- TEST SYSTEM
- Test vessel: 400 mL beaker with 250 mL medium
- Test vessel: open (2,4-DNT), closed (2,6-DNT)
- Renewal rate of test solution (frequency/flow rate): 48 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Artificial medium (DIN 38412, Part II, 1982)
- Na/K Ratio: 10:1
- pH-values and oxygen-concentration were measured during the test in two tests-vessels per concentration level. The detected variation of these
parameters had no negative influence on the organisms.
OTHER TEST CONDITIONS
- Photoperiod: light from 7 a.m. to 4 p.m.
- Light intensity: fluorscent lamps, Philips TL 40/25W
- Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.04 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Test substance: 2,4-DNT
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.02 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Test substance: 2,4-DNT; Estimation based on the recovery rates of higher tested concentrations (detection limit for 2,4-DNT: 0.05 mg/L
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: mortality and reproduction
- Remarks on result:
- other: Test substance: 2,6-DNT
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.06 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- other: mortality and reproduction
- Remarks on result:
- other: Test substance: 2,6-DNT; Estimation based on the recovery rates of higher tested concentrations (detection limit for 2,6-DNT: 0.16 mg/L
Any other information on results incl. tables
Analytical monitoring was performed. But as the NOECs were below the detection limits, the actual concentrations could not be determined. Therefore due to recovery experiments a minimum NOEC was estimated.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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