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EC number: 202-429-0 | CAS number: 95-53-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Additional information
A large number of tests on biodegradability are available for o-toluidine. In the following the tests that were conducted according to standard methods are presented. Several experimental data prove that o-toluidine is readily biodegradable.
The biodegradability of o-toluidine under the conditions described by the OECD TG 301 A and E was tested by 6 different laboratories. All the laboratories followed the same procedure. Based on DOC measurement the following results were obtained after 28 days: with the method OECD TG 301 E, five laboratories obtained > 90 % biodegradation, the other one reported a 67 % value. The results obtained with the method OECD TG 301 A showed biodegradation between 88 and 90 % after 28 days. In all cases the 10 d-window criterion was fulfilled.
According to the national Japanese standard method comparable to the OECD TG 301 C guideline an aerobic ready test was performed. Related to BOD after a period of 28 days 65.4 % biodegradation was observed. No information is given in relation to the 10 d-window.
Results on the inherent biodegradability of o-toluidine are also available.
A test on inherent biodegradability was conducted. The test design is comparable to the Zahn-Wellens-Test (OECD TG 302 B). The test substance o-toluidine in a concentration of 200 mg/L COD was the sole source of carbon. Activated sludge from a sewage treatment plant adapted for 20 days to o-toluidine was used as inoculum in a concentration of 100 mg/L dry matter.
Based on COD measurement, a removal of 97.7 % within 5 days was obtained.
With adapted activated sludge from an industrial sewage treatment plant a test on inherent biodegradation was conducted. The procedure followed the OECD guideline 302 B. After 11 days (4 days adaptation) 96 % of the initial concentration were removed.
Biodegradation in river water was investigated in a simulation experiment. Test filter experiments were carried out in darkness under stable aerobic conditions by circulating water through a glass column filled with pumice. Based on the degradation parameters determined, o-toluidine was classified as biodegradable under the given conditions. Further, in mineral salts medium with and without (NH4)2SO4, complete removal of o-toluidine was achieved after 2 days and after 4 days, respectively. A Gram-positive bacterium (Rhodococcus rhodochrous) with the ability to utilize o-toluidine as sole source of carbon and nitrogen was isolated from soil.
Under anaerobic conditions o-toluidine could not be removed after a period of 10 month in an aquifer slurry. o-Toluidine revealed no biotic transformation, neither under sulfate reducing nor under methanogenic conditions.
In a further test o-toluidine could not be removed after 150 days in a landfill environment under anaerobic conditions. The test was not performed according to current standard methods but indicates that the substance is stable under anaerobic terms.
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