Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-617-8 | CAS number: 123-31-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout (Oncorhynchus mykiss)
- Source: Goosen's Trout Farm, Otterville, ON (Canada)
- Age at study initiation (mean and range, SD): not reported
- Length at study initiation (length definition, mean, range and SD): 4.6-6.4 cm
- Weight at study initiation (mean and range, SD): 1.2-3.8 g
- Feeding during test: not reported
ACCLIMATION
- Acclimation period: at least 7 d
- Acclimation conditions (same as test or not): yes
- Type and amount of food: Ewos trout pellets
- Feeding frequency: daily
- Health during acclimation (any mortality observed): not reported - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- no data
- Test temperature:
- 14.1-16.5°C
- pH:
- 7.6-8.19
- Dissolved oxygen:
- 5.6-9.4 mg/L
- Salinity:
- no data
- Nominal and measured concentrations:
- 0 (control), 10, 18, 32, 56, 100% of the maximum test concentration (not further specified)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: >=14 L tank
- Type: not reported
- Material: not reported
- Size: >=14 L
- Headspace: not reported
- Fill volume: 14 L
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): diluter according to Mount-Brungs (1967)
- Renewal rate of test solution (frequency/flow rate): 21-111 mL/min; 95% replacement time: 6-33 h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Biomass loading rate: 0.8-2.7 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: the water for stock and bioassay tanks was a municipal supply drawn from Lake Ontario. The water was dechlorinated to < 10 µg Cl/L
- Alkalinity: 86 mg/L as CaCO3
- Conductivity: 340 µmhos/cm²
- Culture medium different from test medium: the test medium was the same as during the acclimation period
- Intervals of water quality measurement: not reported
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light/8 h dark
- Light intensity: not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality
TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 1.8
- Range finding study
- Test concentrations: 1, 10, 100, 1000 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.638 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: not reported
- Observations on body length and weight: not reported
- Other biological observations: not reported
- Mortality of control: 1.25% in all aqueous bioassays
- Other adverse effects control: not reported
- Abnormal responses: not reported
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: not reported
- Effect concentrations exceeding solubility of substance in test medium: no - Reported statistics and error estimates:
- Probit analysis or graphical method, multiple regression analysis and chi-square tests
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The acute toxicity of hydroquinone to Oncorhynchus mykiss was investigated in a study conducted similar to OECD Guideline 203. The 96 h LC50 was determined to be 0.638 mg/L (measured).
- Executive summary:
The acute toxicity of hydroquinone to Oncorhynchus mykiss was investigated in a study conducted similar to OECD Guideline 203 (Fish, Acute Toxicity Test). The fish were exposed to 6 concentrations and one control for 96 h under flow-through conditions. The test substance concentrations were measured daily via UV absorbance. The assay method was the measurement of the absorbance of ultraviolet light by the test solutions in a quartz cell with a 1-cm path length. Concentrations were calculated by reference to standard curves of the chemical dissolved in control tank water. For hydroquinone the concentration/calibration curve was not linear within the test concentration range. The wavelength at which the chemical absorbed maximally was identified by scanning a standard solution. Appearance of more than one peak in the test solutions indicated whether or not the compound was degrading during the exposure. No details of the analytical results, however, were reported. Due to the known instability of hydroquinone in aqueous solution this information would have been of great importance. The 96 h LC50 was determined to be 0.638 mg/L (=5.8 µmol/L; measured). Fish length, water temperature, loading, pH and water hardness are in accordance with the recommendation of the current OECD Guideline 203.
This study is regarded as reliable with restrictions due to the missing raw data.
Reference
Description of key information
In a study conducted comparable to OECD Guideline 203 and under flow-through conditions, a 96 h LC50=0.638 mg/L was determined using Oncorhynchus mykiss as test organism.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 0.638 mg/L
Additional information
Three acute tests with two freshwater species are available (Onchorhynchus mykiss, Pimephales promelas) with 96 h LC50 values of 0.044, 0.097 and 0.638 mg/L. Concerning the effects on fish the study of Hodson et al. (1984) and the LC50=0.638 mg/L is preferred because the test performance generally fulfills the requirements of the OECD Guideline 201, contrary to the other two tests.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.