Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 246-356-2 | CAS number: 24613-89-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Published study of non-standard design, however results are valid and the study is well reported.
Data source
Reference
- Reference Type:
- publication
- Title:
- Oral chromium(VI) does not affect the frequency of micronuclei in hematopoietic cells of adult mice and of transplacentally exposed fetuses
- Author:
- De Flora S, Iltcheva M, Balansky RM
- Year:
- 2 006
- Bibliographic source:
- Mutation Research 610(1-2): 38-47
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The study investigated the effects of oral Cr (VI) on micronucleus frequency in bone marrow or peripheral blood erythrocytes of mice.
- GLP compliance:
- not specified
- Remarks:
- published study
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- Potassium dichromate
- EC Number:
- 231-906-6
- EC Name:
- Potassium dichromate
- Cas Number:
- 7778-50-9
- Molecular formula:
- Cr2H2O7.2K
- IUPAC Name:
- potassium dichromate
- Details on test material:
- Potassium dichromate, purchased from Sigma Chemical Co.
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: BDF1
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- BDF1 mice were 8 months old and weighed an average of 34.9 g. Mice were obtained from the Animal Laboratory National Centre of Oncology, Bulgaria. They were housed in plastic cages on sawdust bedding, and maintained on standard rodent chow and tap water ad libitum. The temperature of the room was maintained at 23±2°C, relative humidity was 50-55%, and a 12 hour light/dark cycle. Mice were acclimatised for 10-15 days.
Administration / exposure
- Route of administration:
- other: oral drinking water, gavage and i.p.
- Vehicle:
- None for drinking water groups. Sterile distilled water for gavage and intraperitoneal groups.
- Details on exposure:
- Male mice received potassium dichromate in the drinking water for 20 days at 0, 10 and 20 mg Cr(VI)/L (n=10). Additional groups of 10 mice each received a single oral gavage dose or a single intrapertioneal dose of 50 mg/kg bw on day 19 (equivalent to 17.7 mg Cr(VI)/kg bw).
- Duration of treatment / exposure:
- 20 days
- Frequency of treatment:
- Continuous in drinking water or single dose
- Post exposure period:
- None.
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
0, 10 and 20 mg Cr(VI)/L
Basis:
nominal in water
- Remarks:
- Doses / Concentrations:
17.7 mg Cr(VI)/kg bw
Basis:
nominal conc.
single gavage/i.p dose
- No. of animals per sex per dose:
- 10
- Control animals:
- yes, concurrent no treatment
- Positive control(s):
- Not required.
Examinations
- Tissues and cell types examined:
- Peripheral blood and bone marrow
- Details of tissue and slide preparation:
- Peripheral blood samples were collected from the lateral tail vein of the controls and the mice exposed via the drinking water on days 0, 5, 12 and 20. On day 20 all mice were killed and the left femurs removed and dissected for collection of bone marrow cells.
Immediately after collection, duplicate smears of peripheral blood were preprared, air-dried and stained with May-Grunwald-Giemsa. From each mouse, 40000 normochromatic erythrocytes (NCE) were scored for the presence of micronucleated cells.
Immediately after collection duplicate smears of bone marrow cells were prepared, air-dried, and stained with May-Grunwald-Giemsa. 2000 polychromatic erythrocytes (PCE) per mouse were scored for the presence of micronucleated cells. 200 NCE and the corresponding PCE were scored for determining the PCE/NCE ratio. - Statistics:
- Student's t-test and ANOVA for repeated measures.
Results and discussion
Test resultsopen allclose all
- Sex:
- male
- Genotoxicity:
- negative
- Remarks:
- oral exposure
- Toxicity:
- no effects
- Vehicle controls validity:
- valid
- Negative controls validity:
- not applicable
- Positive controls validity:
- not applicable
- Sex:
- male
- Genotoxicity:
- positive
- Remarks:
- intraperitoneal exposure
- Toxicity:
- yes
- Additional information on results:
- Administration of potassium dichromate in the drinking water for 20 days had no effect on the frequency of micronucleated PCE or the PCE/NCE ratio in bone marrow. There was also no effect on the frequency of micronucleated NCE in peripheral blood. Similarly, a single gavage dose of 17.7 mg Cr(VI)/kg bw had no effect on the frequency of micronucleated PCE or the PCE/NCE ratio in bone marrow.
When administered i.p., potassium dichromate caused an increase in the frequency of micronucleated PCE in bone marrow, but did not affect the PCE/NCE ratio.
Any other information on results incl. tables
There were no effects on body weight change in mice exposed via the drinking water. The daily intakes of chromium were calculated to be approximately 3 and 6 mg/kg bw in the mice drinking water containing 10 and 20 mg Cr (VI)/L, respectively.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): other: genotoxicity is dependent on route of exposure
Orally adminstered Cr (VI) is adequately detoxified in the gastrointestinal tract before it exerts genotoxic effects, however bypassing the detoxification mechanisms (intraperitoneal route of exposure) results in positive mutagenic responses. - Executive summary:
Potassium dichromate did not affect the frequency of micronucleated polychromatic erythrocytes when administered orally to male mice. However potassium dichromate significantly increased the frequency micronucleated polychromatic erythrocytes when administered intraperitoneally. The authors concluded that orally administered Cr (VI) is adequately detoxified in the gastrointestinal tract before it exerts genotoxic effects, however bypassing the detoxification mechanisms (intraperitoneal route of exposure) results in positive mutagenic responses.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.