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EC number: 202-267-0 | CAS number: 93-68-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study documented and performed according to GLP standards and in compliance with OECD Test Guidelines 471 and 472.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)
- Deviations:
- not specified
- Principles of method if other than guideline:
- Not applicable.
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2'-methylacetoacetanilide
- EC Number:
- 202-267-0
- EC Name:
- 2'-methylacetoacetanilide
- Cas Number:
- 93-68-5
- Molecular formula:
- C11H13NO2
- IUPAC Name:
- N-(2-methylphenyl)-3-oxobutanamide
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Purity: 99.9%
Constituent 1
Method
- Target gene:
- Not applicable.
Species / strain
- Species / strain / cell type:
- other: TA98, TA100, TA1535, TA1537, Escherichia coli WP2uvrA
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 from male rat liver, induced with phenobarbital and 5,6-benzoflavon
- Test concentrations with justification for top dose:
- 0, 156, 313, 625, 1250, 2500 and 5000 microg/plate.
- Vehicle / solvent:
- Dimethylsulphoxide
Controls
- Untreated negative controls:
- yes
- Remarks:
- dimethylsulphoxide
- Negative solvent / vehicle controls:
- yes
- Remarks:
- dimethylsulphoxide
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Without S9mix: 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (TA 98, TA 100 and WP2), sodium azide (TA 1535), 9-aminoacridine hydrochloride (TA 1537); With S9 mix: 2-aminoanthracene (all five strains)
- Details on test system and experimental conditions:
- TEST SYSTEM:
Metabolic activation system: S9 from male rat liver, induced with phenobarbital and 5,6-benzoflavon
ADMINISTRATION:
Number of replicate: 2
Plates per dose: 3
Application: Pre-incubation
Solvent: DMSO (Concentration was not described)
Test parameter: Revertant colonies per plate - Evaluation criteria:
- Number of revertant colonies for all treatment groups is compared with those obtained for solvent control groups.
Any toxic effects of the test substance can be detected by a substantial reduction in revertant colony counts or by
the absence of a complete background bacterial lawn. - Statistics:
- Not applicable.
Results and discussion
Test results
- Species / strain:
- other: TA98, TA100, TA1535, TA1537, Escherichia coli WP2uvrA
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- Number of revertant colonies per plate in all doses with/without S9mix were equivalent to control. On the other hand, more than two times revertant colonies were observed in all positive controls. Visible precipitation was not observed in any plates.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative without metabolic activation
negative with metabolic activation
lt is concluded that, when tested at dose levels up to 5000 microg/plate in dimethylsulphoxide, Acetoacet-o-toluidide was not mutagenic in this bacterial test system. - Executive summary:
The genetic toxicity study (Ames) was published in the OECD SIDS dossier in year 2003 conducted and reported
according to the OECD Guideline for the testing of chemicals 471 and 472 and principles of GLP in year 1999. Number of revertant colonies per plate in all doses with/without S9 mix were equivalent to control. On the other hand, more than two times revertant colonies were observed in all positive controls. Visible precipitation was not observed in any plates.When tested at dose levels of up to 5000 microg/plate in five strains with or without S9 mix no toxicity was observed. A top dose level of 5000 g/plate was chosen for the subsequent mutation study. Other dose levels used in the mutation assays were: 2500, 1250, 625, 313, 156 microg/plate. The concurrent positive control compounds demonstrated the sensitivity of the assay and the metabolising activity of the liver preparations.
Therefore, Acetoacet-o-toluidide is not mutagenic to Salmonella typhimurium and Escherichia coli.
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