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EC number: 241-677-4 | CAS number: 17689-77-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 1978-01-18 to 1979-11-15
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The study was conducted according to a test protocol that is comparable to the appropriate OECD test guideline, with acceptable restrictions. It was not compliant with GLP. The restrictions are that only 100 cells were analysed per concentration. Read-across to the registered substance is considered scientifically justified.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- Deviations:
- yes
- Remarks:
- only 100 cells were analysed per concentration
- GLP compliance:
- not specified
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- Triethoxy(methyl)silane
- EC Number:
- 217-983-9
- EC Name:
- Triethoxy(methyl)silane
- Cas Number:
- 2031-67-6
- IUPAC Name:
- triethoxy(methyl)silane
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor induced rat liver S9
- Test concentrations with justification for top dose:
- 0.125-2.0 µl/m
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: ethanol
- Justification for choice of solvent/vehicle: It is assumed by the reviewer that the solvent was chosen for its solubility properties and relative non-toxicity to bacteria
Controls
- Untreated negative controls:
- yes
- Remarks:
- Media
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- ethylmethanesulphonate
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium
DURATION
- Exposure duration: 4 hours
- Expression time (cells in growth medium): 24 hours
- Fixation time (start of exposure up to fixation or harvest of cells): 25 hours
SPINDLE INHIBITOR (cytogenetic assays): Colcemid
STAIN (for cytogenetic assays): 10 % Giemsa
NUMBER OF REPLICATIONS: 1
NUMBER OF CELLS EVALUATED: 100 cells per test conc.
DETERMINATION OF CYTOTOXICITY
- Method: Toxicity was measured as loss in growth potential of the cells induced by a 4-hour exposure to the test article followed by a 24-hour expression period in growth medium
- Evaluation criteria:
- The test substance is clastogenic if a statistically significant dose related increase in aberrations is shown.
- Statistics:
- A two-tailed t-test (Finney 1971) was used to determine statistical significance of results. Clearly positive results indicated by p < 0.01
Results and discussion
Test results
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: strain/cell type: mouse lymphoma L5178Y cells
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 2: Results of chromosome analysis Experiment 1 without activation (per 100 cells scored)
|
Solvent Control |
Negative Control |
Positive Control |
0.125 μl/ml |
0.25 μl/ml |
0.5 μl/ml |
1 μl/ml |
2 μl/ml |
|
Cytotoxicity |
No |
No |
Yes |
No |
No |
No |
No |
No |
|
|
|||||||||
Chromatid aberrations |
gaps |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
breaks |
0 |
0 |
4 |
0 |
0 |
0 |
0 |
0 |
|
interchanges |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
deletion |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
Chromosome aberrations |
gaps |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
breaks |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
interchanges |
0 |
0 |
0 |
0 |
2 |
0 |
0 |
0 |
|
acentric fragment |
4 |
3 |
18 |
1 |
11 |
5 |
2 |
1 |
|
pulverised Cells |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
pulverised chromosome |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
triradial |
0 |
0 |
11 |
0 |
1 |
0 |
0 |
1 |
|
quadriradial |
0 |
0 |
10 |
0 |
0 |
0 |
0 |
0 |
|
ring |
1 |
0 |
0 |
0 |
1 |
1 |
0 |
0 |
|
fragment |
2 |
0 |
5 |
4 |
2 |
6 |
1 |
0 |
|
deletion |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
|
diacentric chromosome |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
minute chromosome |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
1 |
|
complex rearrangement |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
Mitotic index (%) |
7 |
7.4 |
4.4 |
6.8 |
7.4 |
6.8 |
5.8 |
5.6 |
|
Polyploidy (% mean freq.) |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
Endo reduplication |
NR |
NR |
NR |
NR |
NR |
NR |
NR |
NR |
Solvent control with Ethanol
NR not reported
Table 3: Results of chromosome analysis Experiment 1 with activation (per 100 cells scored)
|
Solvent Control |
Negative Control |
Positive Control |
0.125 μl/ml |
0.250 μl/ml |
0.5 μl/ml |
1 μl/ml |
2 μl/ml |
|
Cytotoxicity |
No |
No |
Yes |
No |
No |
No |
No |
No |
|
|
|||||||||
Chromatid aberrations |
gaps |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
breaks |
0 |
0 |
3 |
0 |
0 |
0 |
0 |
0 |
|
interchanges |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
deletion |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
|
Chromosome aberrations |
gaps |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
breaks |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
interchanges |
0 |
0 |
3 |
0 |
0 |
0 |
0 |
0 |
|
acentric fragment |
2 |
4 |
3 |
6 |
7 |
5 |
10 |
4 |
|
pulverised Cells |
0 |
0 |
27 |
0 |
0 |
1 |
0 |
0 |
|
pulverised chromosome |
0 |
0 |
4 |
0 |
0 |
0 |
0 |
0 |
|
triradial |
2 |
0 |
2 |
0 |
0 |
2 |
3 |
1 |
|
quadriradial |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
|
ring |
0 |
0 |
1 |
0 |
0 |
1 |
1 |
0 |
|
fragment |
4 |
0 |
2 |
4 |
4 |
9 |
8 |
3 |
|
deletion |
0 |
0 |
0 |
0 |
2 |
0 |
0 |
0 |
|
diacentric chromosome |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
minute chromosome |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
0 |
|
complex rearrangement |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
|
Mitotic index (%) |
6.8 |
6.4 |
0.8 |
7.8 |
6.6 |
10.8 |
8.6 |
5.4 |
|
Polyploidy (% mean freq.) |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
Endo reduplication |
NR |
NR |
NR |
NR |
NR |
NR |
NR |
NR |
Solvent control with Ethanol
NR not reported
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without metabolic activation
Triethoxy(methyl)silane has been tested according to a protocol that is similar to OECD 473. The test substance did not cause a statistically significant, dose related increase in chromosome aberrations. The test substance was considered non-clastogenic in mouse lymphoma L5178Y cells under the conditions of the test.
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