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EC number: 212-298-1 | CAS number: 778-94-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02 Mar – 01 May 2019
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Version / remarks:
- 2012
- GLP compliance:
- yes
- Remarks:
- The test was performed in compliance with the requirements of the Principles of Good Laboratory Practices (GB/T 22278-2008).
Test material
- Reference substance name:
- 2-nitro-4-(trifluoromethyl)benzonitrile
- EC Number:
- 212-298-1
- EC Name:
- 2-nitro-4-(trifluoromethyl)benzonitrile
- Cas Number:
- 778-94-9
- Molecular formula:
- C8H3F3N2O2
- IUPAC Name:
- 2-nitro-4-(trifluoromethyl)benzonitrile
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Control, 0.1, 1.6 mg/L
- Sampling method: At the start of the actual test, day 0 (fresh) to day 3 (valid), day 9 (fresh) to day 12 (valid), and day 18 (fresh) to day 21 (valid), the highest (1.6 mg/L) and the lowest concentrations (0.1 mg/L) of test substance solution concentrations were measured. Therefore, 20 mL of the treatment with a test substance concentration of 0.1 mg/L and 10 mL of the treatment with a test substance concentration of 1.6 mg/L were sampled.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 0.0101 g - 0.0102 g of the test substance NTBN was weighed and added to 100 mL Elendt M7, and dissolved fully. Different concentrations of NTBN test solution were obtained by dilution with Elendt M7; at the same time, a blank control group was established.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: Batch number: D20190410D
- Age at study initiation (mean and range, SD): less than 24 h old
- Source: Nanjing Institute of Environmental Sciences of the Ministry of Environmental Protection
- Feeding during test: yes
- Food type: Feeding was with green algae (Chlorella vulgaris Beij.) liquid concentrate
- Amount: 0.1-0.2 mg C/Daphnia each time
- Frequency: once daily
- Other information: For each testing round, the daphnia used were identical healthy parthenogenic female offspring daphnia of three generations or more cultivated in the laboratory
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- Hardness:
- 205 - 226 mg (CaCO3)/L
- Test temperature:
- 20.0 - 20.4 °C
- pH:
- 6.91 - 7.48
- Dissolved oxygen:
- 7.20 - 8.43 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 0 (Control), 0.1, 1.6 mg/L
Mean measured concentrations: < LOD/LOQ, 0.0971, 1.58 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Beaker (100 mL, Tianbo Instruments Factory, with breathable lid)
- Type (delete if not applicable): open
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): Every 72 h.
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: Once a week, dissolved oxygen concentration, temperature, hardness, and pH value were measured in the fresh solution and old solution of the control group and the lowest and highest test concentration groups.
OTHER TEST CONDITIONS
- Photoperiod: 16 h light per day
- Light intensity: 1000 lux to 1500 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Offspring: In the test period, surviving offspring Daphnia in each treatment group and control group were observed and counted daily.
- Other test parameters: First time parent Daphnia produced offspring (and subsequent times that offspring were produced) and the number of batches of offspring produced by each parent Daphnia and quantities per batch were recorded.
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 0.0 (Control), 1.00 mg/L and 10.0 mg/L
- Results used to determine the conditions for the definitive study: 100% of offspring survived at 1.00 mg/L; 0% of mother daphnia survived at 10 mg/L. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 1.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 1.58 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.58 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Mortality of control: At the end of the test, there were no deaths of mother Daphnia in the blank control group and the test groups.
- Immobilisation of control: Over the test period, Daphnia magna in the control group exhibited normal activity and each surviving parent Daphnia successfully produced 96 - 127 live offspring Daphnia, the coefficient of variation for reproduction quantity for each parent Daphnia was 9.80% (less than 25%).
- Reproduction: The difference in mother Daphnia reproduction rates between the 0.80 mg/L test group and the control group was significant (p < 0.05).
Any other information on results incl. tables
Table 1: Measurement Results for Test Solution Stability in Test Medium
Prepared concentration (mg/L) |
Actual measured concentration (mg/L) |
||||||
d 0 |
d 3 |
d 9 |
d 12 |
d 18 |
d 21 |
Mean |
|
Fresh |
Valid |
Fresh |
Valid |
Fresh |
Valid |
||
Blank control |
ND |
ND |
ND |
ND |
ND |
ND |
- |
0.1 |
0.0976 |
0.0931 |
0.103 |
0.0949 |
0.101 |
0.0931 |
0.0971 |
1.6 |
1.57 |
1.56 |
1.60 |
1.58 |
1.63 |
1.56 |
1.58 |
ND: Lower than the method’s limit of detection.
Table 2: Actual Test Reproduction Rate Inhibitory Effects
Prepared concentration (mg/L) |
Initial number of Daphnia (n) |
21 d |
|
Mean reproduction quantity (number of surviving offspring Daphnia/ mother Daphnia) |
Inhibition rate (%) |
||
Blank control |
10 |
106 |
- |
0.1 |
10 |
101 |
4.72 |
0.2 |
10 |
99 |
6.60 |
0.4 |
10 |
88 |
17.0 |
0.8 |
10 |
82 |
22.6 |
1.6 |
10 |
41 |
61.3 |
LOEC (Dunnett's Test) |
0.80 mg/L (prepared concentration) |
||
NOEC (Dunnett's Test) |
0.40 mg/L (prepared concentration) |
Table 3: Validity criteria for OECD 211
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The mortality of the parent animals in the control (female Daphnia) does not exceed 20% at the end of the test. |
At the end of the test, 100% of the parent animal in the blank control group survived and activity was normal. |
yes |
The mean number of living offspring produced per parent animal surviving at the end of the test is ≥ 60 in the control. |
Each surviving parent Daphnia successfully produced 96 - 127 (mean reproduction quantity: 106 surviving offspring) live offspring Daphnia, the coefficient of variation for reproduction quantity among parent Daphnia was 9.80%. |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- See Table 3 in "Any other information on results incl. tables".
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