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EC number: 204-853-1 | CAS number: 127-63-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2021
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Diphenyl sulphone
Lot nr 202011220005
Diphenyl sulphone content=99.99 % - Analytical monitoring:
- yes
- Details on sampling:
- Samples for possible analysis were taken from all concentrations and the control.
Frequency at t=0 h and t=96 h
Volume 1.4 mL
Storage Samples were stored in a freezer (set to maintain -20°C) until analysis at the analytical laboratory of the Test Facility.
Additionally, reserve samples of 1.4 mL were taken from all test solutions for possible analysis. If not already used, these samples were stored in a freezer (set to maintain -20°C) for a maximum of three months after delivery of the draft report, pending on the decision of the sponsor for additional analysis. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna (Crustacea, Cladocera) (Straus, 1820), at least third generation, obtained by a cyclical parthenogenesis under specified breeding conditions.
Start of each batch :Approximately 250 newborn daphnids, i.e. less than 3 days old, were placed into 5 litres of medium in an all-glass culture vessel.
Maximum age of the cultures 4 weeks
Renewal of the culture, after 7 days of cultivation, half of the medium twice a week. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 180mg/L as CaCo3
- Test temperature:
- 18-22°C
- pH:
- 6-+9
- Dissolved oxygen:
- >= 3mg/L
- Nominal and measured concentrations:
- measured concentrations
- Details on test conditions:
- TEST SYSTEM
- Test vessel:60 ml all glass
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: glass
- Volume of solution:50 ml
- Aeration: no Aeration
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates):2
- No. of vessels per vehicle control (replicates): no vehicle
- Biomass loading rate:100mg/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:to tap water purified by Reverse Osmosis (RO-water, GEON Waterbehandeling, Berkel-Enschot, The Netherlands
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:<10µS/cm
- Salinity:
- Culture medium different from test medium:
- Intervals of water quality measurement: pH and O2 At the beginning and at the end of the test, for all concentrations and the control.Temperature Continuously, in a temperature control vessel.
OTHER TEST CONDITIONS
- Adjustment of pH: no adjustments no
- Photoperiod:A daily photoperiod of 16 hours
- Light intensity:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
VEHICLE CONTROL PERFORMED: yes/no
RANGE-FINDING STUDY
- Test concentrations: • Ten daphnids per concentration (in duplicate, 5 per vessel) were exposed to solutions containing 0.10, 1.0 and 10% of the SS prepared at a loading rate of 100 mg/L.
- Results used to determine the conditions for the definitive study:No immobility was observed in the control and the three lowest test concentrations throughout the test. At the end of the test, all daphnids exposed to the highest test concentration were immobilized - Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 15 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In conclusion, the 48h-EC50 for Daphnia magna exposed to Diphenyl sulphone was 15 mg/L based on geometric mean concentrations (95% confidence interval between 14 and 17 mg/L).
- Executive summary:
The objective of the study was to evaluate Diphenyl sulphone for its ability to generate acute toxic effects on the mobility of Daphnia magna during an exposure period of 48 hours and, if possible, to determine the EC50 at 24 and 48 hours of exposure.
A full test was performed based on the results of a preceding combined limit/range-finding test. The study met the acceptability criteria prescribed by the study plan and was considered valid. Test conditions and results of the final test are presented in the table below:
Test Item
Diphenyl sulphone
Appearance
White crystalline powder
Purity
99.99%
Preparation of test solutions
Saturated Solution (SS), loading rate of 100 mg/L.
Lower concentrations: serial dilution.
Experimental Set-up
Guidelines
OECD TG 202 and OECD GD 23
Exposure design
Static, no renewals
Test concentrations
Solutions containing 10, 18, 32, 56 and 100% of the SS prepared at a loading rate of 100 mg/L
Control
Blank control
Number of replicates
4 replicates containing 5 daphnids each
Sampling for analysis
At t=0, and t=48h.
Experimental conditions
pH, Temperature, Dissolved Oxygen
Within the ranges specified in OECD TG 202
Light regime
A daily photoperiod of 16 hours
Results
Actual exposure concentrations
2.8, 4.9, 8.6, 16 and 29 mg/L (geometric mean concentrations)
Recorded effects
No immobility was observed in the control and three lowest test concentrations throughout the exposure period. The two highest concentrations resulted in 60% and 100% immobility at the end of the test, respectively.
The effect parameters (based on geometric mean concentrations) obtained in this study are summarized in the table below.
Parameter
Diphenyl sulphone
Geometric mean conc. (mg/L)
95%-confidence interval (mg/L)
24h-EC50
21
18-24
48h-EC50
15
14-17
Reference
Description of key information
The 48-hr LC50 value for Daphnid is 15 mg/l (OECD 202).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 15 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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